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Product Info Summary
| SKU: | EK0747 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Mouse DAN/NBL1 ELISA Kit PicoKine®
SKU/Catalog Number
EK0747
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse DAN/NBL1 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Nbl1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse DAN/NBL1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0747)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: A17-D178
Sensitivity
<10 pg/ml
Assay Range
62.5 pg/ml - 4,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0747 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0747 is reactive to Nbl1 in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0747 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of DAN/NBL1
Differential screening-selected gene aberrative in neuroblastoma (DAN) is a member of the DAN family of secreted glycoproteins that are putative BMP antagonists. The NBL1 gene, also known as DAN, is originally cloned from a normal rat fibroblast cDNA library by a differential screening method. The human DAN gene is mapped to chromosome 1p36.13-p36. It is found that the DAN gene possesses a tumor suppressive activity when overexpressed in v-src transformed cells.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0747-CAP | Anti-Mouse Nbl1 Pre-coated 96-well strip microplate | 1 |
| EK0747-ST | Mouse Nbl1 Standard | 2 vials, 10 ng/tube |
| EK0747-DA | Mouse Nbl1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.049 | 0.075 | 0.104 | 0.204 | 0.464 | 0.838 | 1.386 | 2.028 |
Data Example Images
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Mouse DAN/NBL1 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect DAN/NBL1, Dilution ratio of 1:1, concentration in serum and plasma is around 2.8 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse DAN/NBL1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 84 | 591 | 1510 | 86 | 596 | 1475 |
| Standard deviation | 5.54 | 31.91 | 78.52 | 6.7 | 37.54 | 89.97 |
| CV (%) | 6.6% | 5.4% | 5.2% | 7.8% | 6.3% | 6.1% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of DAN/NBL1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 84 | 77 | 79 | 90 | 82 | 5.02 | 6.1% |
| Sample 2 | 591 | 589 | 657 | 668 | 626 | 36.46 | 5.8% |
| Sample 3 | 1510 | 1598 | 1670 | 1616 | 1598 | 57.55 | 3.6% |
Protein Target Info & Infographic
Gene/Protein Information For Nbl1 (Source: Uniprot.Org, NCBI)
Gene Name
Nbl1
Full Name
Neuroblastoma suppressor of tumorigenicity 1
Weight
19107 MW
Superfamily
DAN family
Alternative Names
Neuroblastoma suppressor of tumorigenicity 1;N03;Zinc finger protein DAN;Nbl1;Dan, Dana; NBL1 D1S1733E, DAN, DAND1, NB, NO3 NBL1, DAN family BMP antagonist neuroblastoma suppressor of tumorigenicity 1|DAN domain family member 1|differential screening-selected gene aberrant in neuroblastoma|neuroblastoma 1, DAN family BMP antagonist|neuroblastoma candidate region, suppression of tumorigenicity 1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Nbl1, check out the Nbl1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Nbl1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse DAN/NBL1 ELISA Kit PicoKine® (EK0747)
Hello CJ!
EK0747 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Inhibition by microbial metabolites of Chinese dark tea of age-related neurodegenerative disorders in senescence-accelerated mouse prone 8 (SAMP8) mice
Mesona chinensis Benth polysaccharides protect against oxidative stress and immunosuppression in cyclophosphamide-treated mice via MAPKs signal transduction pathways
Phase 2, randomized, double%u2010blind, placebo%u2010controlled, multicenter clinical evaluation of recombinant human thrombin in multiple surgical indications
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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9 Customer Q&As for Mouse DAN/NBL1 ELISA Kit PicoKine®
Question
Does EK0747 contain native human biological materials?
Verified customer
Asked: 2021-11-10
Answer
The Mouse DAN/NBL1 ELISA Kit PicoKine® (EK0747) doesn't contain native human biological materials.
Boster Scientific Support
Answered: 2021-11-11
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring DAN using the EK0747 Mouse DAN Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2020-07-10
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to limit protein degradation.
Boster Scientific Support
Answered: 2020-07-10
Question
Q: can you suggest the dilution ratio of serum samples for detection of DAN in Mouse plasma? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.
M. Martinez
Verified customer
Asked: 2020-03-27
Answer
A: having little idea about the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the DAN ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2020-03-27
Question
Q: Can DAN ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
W. Baker
Verified customer
Asked: 2019-12-08
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is needed. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2019-12-08
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2019-06-01
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer may affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2019-06-01
Question
Q: What is the optimal O.D. value for DAN ELISA kit? I performed your DAN ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain DAN even though the O.D. values are not very high?
A. Lopez
Verified customer
Asked: 2018-10-02
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. considering your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this DAN ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of DAN that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2018-10-02
Question
Q: can I use citrate plasma as samples in Mouse DAN Picokine® ELISA Kit (Catalog # EK0747)?
Verified Customer
Verified customer
Asked: 2018-07-06
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of DAN causing degradation of its protein structure. DAN may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the DAN ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2018-07-06
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
T. Robinson
Verified customer
Asked: 2015-06-08
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2015-06-08
Question
Q: how to proceed with the analysis of ELISA data? I have obtained DAN level in plasma.
W. Zhou
Verified customer
Asked: 2014-07-12
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2014-07-12
