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Product Info Summary
| SKU: | EKC1310 |
|---|---|
| Size: | 1 kit, containing one 96-well plate and all necessary reagents |
| Reactive Species: | Human, Mouse |
| Application: | ELISA |
| Sample Types: | Cell lines |
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Product info
Product Name
ITCH Colorimetric Cell-Based ELISA Kit
SKU/Catalog Number
EKC1310
Size
1 kit, containing one 96-well plate and all necessary reagents
Description
The ITCH Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor ITCH protein expression profile in cells. The kit can be used for measuring the relative amounts of ITCH in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on ITCH.
Storage & Handling
Store at 4°C for up to 6 months.
Cite This Product
ITCH Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC1310)
Clonality of Antibodies
See Datasheet for details
Assay Range
> 5000 cells/well
Reactive Species
EKC1310 is reactive to ITCH in Human, Mouse samples
Validated Sample Types
Cell lines
Application Guarantee
EKC1310 is guaranteed for ELISA in Human, Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
| Reagent | Quantity | Container |
|---|---|---|
| 96-Well Cell Culture Clear-Bottom Microplate | 1 Plates | - |
| 10x TBS | 24 ml (10x) | Clear |
| Quenching Buffer | 24 ml (1x) | Clear |
| Blocking Buffer | 50 ml (1x) | Clear |
| 15x Wash Buffer | 50 ml (15x) | Clear |
| 100x Anti-ITCH Antibody (Rabbit Polyclonal) | 60 µl (100x) | Purple |
| 100x Anti-GAPDH Antibody (Mouse Monoclonal) | 60 µl (100x) | Green |
| HRP-Conjugated Anti-Rabbit IgG Antibody | 6ml (1x) | Glass |
| HRP-Conjugated Anti-Mouse IgG Antibody | 6ml (1x) | Glass |
| Primary Antibody Diluent | 12 ml (1x) | Clear |
| Ready-to-Use Substrate | 12 ml (1x) | Brown |
| Stop Solution | 12 ml (1x) | Clear |
| Crystal Violet Solution | 6ml (1x) | Glass |
| SDS Solution | 24 ml (1x) | Clear |
| Adhesive Plate Seals | 2 Seals | - |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
- Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
- 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
- Deionized or sterile water
- Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
- Graph paper or computer software capable of generating or displaying logarithmic functions
- Absorbent papers or vacuum aspirator
- Test tubes or microfuge tubes capable of storing 1 ml
- Orbital shaker
- Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
Data Examples, Quality Control Data
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Boster Kit Box
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Western blot analysis of extracts from mouse brain cells
Protein Target Info & Infographic
Gene/Protein Information For ITCH (Source: Uniprot.Org, NCBI)
Gene Name
ITCH
Full Name
E3 ubiquitin-protein ligase Itchy
Weight
102803 MW
Alternative Names
AIF4;AIP4;atrophin-1-interacting protein 4;Itchy homolog E3 ubiquitin protein ligase;NAPP1;NFE2-associated polypeptide 1;ubiquitin protein ligase ITCH Itch|6720481N21Rik, 8030492O04Rik, A, A130065M08, AIP4, C230047C07Rik|itchy, E3 ubiquitin protein ligase|E3 ubiquitin-protein ligase Itchy|HECT-type E3 ubiquitin transferase Itchy homolog
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on ITCH, check out the ITCH Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for ITCH: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For ITCH Colorimetric Cell-Based ELISA Kit (EKC1310)
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