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Product Info Summary
| SKU: | EK0684 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human CCL17/TARC ELISA Kit PicoKine®
View all CCL17/TARC ELISA kits
SKU/Catalog Number
EK0684
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human CCL17/TARC ELISA Kit PicoKine® (96 Tests). Quantitate Human CCL17 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human CCL17/TARC ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0684)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: A24-S94
Sensitivity
<1 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0684 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0684 is reactive to CCL17 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0684 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CCL17/TARC
Chemokine (C-C motif) ligand 17 (CCL17) is a small cytokine belonging to the CC chemokine family that is also known as thymus and activation regulated chemokine (TARC). CCL17 is expressed constitutively in thymus, but only transiently in phytohemagglutinin-stimulated peripheral blood mononuclear cells.1 This chemokine specifically binds and induces chemotaxis in T cells and elicits its effects by interacting with the chemokine receptor CCR4.1,2The gene for CCL17 is located on chromosome 16, in humans, along with other chemokines called CCL22 and CX3CL1.3,4 The standard used in this kit is recombinant human CCL17, consisting of 71 amino acids with the molecular weight of 8Kda.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0684-CAP | Anti-Human CCL17 Pre-coated 96-well strip microplate | 1 |
| EK0684-ST | Human CCL17 Standard | 2 vials, 10 ng/tube |
| EK0684-DA | Human CCL17 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.022 | 0.111 | 0.163 | 0.335 | 0.740 | 1.176 | 1.766 | 2.072 |
Data Example Images
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Human CCL17/TARC PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect CCL17/TARC, Dilution ratio of 1:1, concentration in serum is 41-678 pg/ml..
Some articles we found to cite concentrations of CCL17/TARC in samples: 25555269 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human CCL17/TARC ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 62 | 251 | 939 | 56 | 234 | 931 |
| Standard deviation | 2.6 | 10.79 | 53.52 | 2.68 | 12.16 | 62.37 |
| CV (%) | 4.2% | 4.3% | 5.7% | 4.8% | 5.2% | 6.7% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of CCL17/TARC in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 62 | 58 | 60 | 57 | 59 | 1.92 | 3.2% |
| Sample 2 | 251 | 287 | 247 | 277 | 265 | 16.93 | 6.3% |
| Sample 3 | 939 | 986 | 993 | 986 | 976 | 21.55 | 2.2% |
Protein Target Info & Infographic
Gene/Protein Information For CCL17 (Source: Uniprot.Org, NCBI)
Gene Name
CCL17
Full Name
C-C motif chemokine 17
Weight
10507 MW
Superfamily
intercrine beta (chemokine CC) family
Alternative Names
C-C motif chemokine 17;CC chemokine TARC;Small-inducible cytokine A17;Thymus and activation-regulated chemokine;CCL17;SCYA17, TARC; CCL17 A-152E5.3, ABCD-2, SCYA17, TARC C-C motif chemokine ligand 17 C-C motif chemokine 17|CC chemokine TARC|T cell-directed CC chemokine|chemokine (C-C motif) ligand 17|small inducible cytokine subfamily A (Cys-Cys), member 17|small-inducible cytokine A17|thymus and activation-regulated chemokine
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CCL17, check out the CCL17 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CCL17: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human CCL17/TARC ELISA Kit PicoKine® (EK0684)
Hello CJ!
EK0684 has been cited in 8 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Serum cytokine and chemokine profiles in patients with alopecia areata
Natural antiviral compound silvestrol modulates human monocyte‐derived macrophages and dendritic cells
The Expression and Regulation of Interleukin-33 in Human Epidermal Keratinocytes: A New Mediator of Atopic Dermatitis and Its Possible Signaling Pathway
miR-181a Induces Macrophage Polarized to M2 Phenotype and Promotes M2 Macrophage-mediated Tumor Cell Metastasis by Targeting KLF6 and C/EBPα
Distinct mucosal immunopathologic profiles in atopic and nonatopic chronic rhinosinusitis without nasal polyps in Central China
Distinct mucosal immunopathologic profiles in atopic and nonatopic chronic rhinosinusitis without nasal polyps in Central China
Distinct mucosal immunopathologic profiles in atopic and nonatopic chronic rhinosinusitis without nasal polyps in Central China
miR-181a Induces Macrophage Polarized to M2 Phenotype and Promotes M2 Macrophage-mediated Tumor Cell Metastasis by Targeting KLF6 and C/EBP?
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Customer Reviews
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Customer Q&As
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Submit your question
8 Customer Q&As for Human CCL17/TARC ELISA Kit PicoKine®
Question
We
Verified Customer
Verified customer
Asked: 2020-03-31
Answer
We tested recombinant mouse CCL17 protein using EK0684 and no significant cross-reactivity was observed.
Boster Scientific Support
Answered: 2020-03-31
Question
Was EK0684 tested for cross reactivity with CCL17 in any other species besides human?
Verified customer
Asked: 2019-11-07
Answer
Yes, we tested the Human CCL17/TARC ELISA Kit PicoKine® (EK0684) with recombinant mouse CCL17 protein and no significant cross-reactivity was observed.
Boster Scientific Support
Answered: 2019-11-11
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
S. Banerjee
Verified customer
Asked: 2019-02-21
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-02-21
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-11-29
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2018-11-29
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
G. Williams
Verified customer
Asked: 2018-03-14
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2018-03-14
Question
Q: is it okay to use citrate plasma as samples in Human CCL17 Picokine® ELISA Kit (Catalog # EK0684)?
W. Liu
Verified customer
Asked: 2017-05-16
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of CCL17 causing disruption of its protein structure. CCL17 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the CCL17 ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2017-05-16
Question
Q: What is the optimal O.D. value for CCL17 ELISA kit? I used your CCL17 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain CCL17 even though the O.D. values are not very high?
S. Harris
Verified customer
Asked: 2016-11-29
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your protocol, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this CCL17 ELISA kit to have sensitivity of 1pg/ml, that means the minimum amount of CCL17 that can be declared/interpreted as positive by the above standard is 1pg/ml.
Boster Scientific Support
Answered: 2016-11-29
Question
Q: if the enzyme conjugated CCL17 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CCL17 antigen?
M. Patel
Verified customer
Asked: 2016-03-19
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2016-03-19
