Anti-Phospho-Dok-2 (Y299) Antibody

Product Name

Anti-Phospho-Dok-2 (Y299) Antibody

View all DOK2 Antibodies

SKU/Catalog Number

P07956-1

Size

100μl

Form

Liquid

Description

Boster Bio Anti-Phospho-Dok-2 (Y299) Antibody catalog # P07956-1. Tested in WB applications. This antibody reacts with Human, Monkey, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Phospho-Dok-2 (Y299) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P07956-1)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human Dok-2 around the phosphorylation site of Y299.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

P07956-1 is reactive to DOK2 in Human, Monkey, Mouse

Applications

P07956-1 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

52 kDa

Calculated molecular weight

45379 MW

Background of DOK2

XIRP1 is a member of the Xin family of proteins, containing 16 Xin repeats. The intercalated disk protein Xin protects actin filaments from depolymerization, and is able to bundle actin filaments to interact with β-catenin, which is thought to stabilize actin-based cytoskeletons (Choi et al., 2007). The evolutionary emergence of the Xin paralogs may have played a key role in the development of heart chambers with complete endothelial and myocardial layers (Grosskurth et al., 2008). Loss of mXina (mouse Xin homolog) results in cardiac hypertrophy and cardiomyopathy (Gustafson-Wagner et al., 2007). Phosphorylation of Ser-295 plays a key role in XIRP1 signaling, and therefore in cardiac hypertrophy, as it has been shown that acute pressure-overloaded hearts rapidly increased phosphorylation at the Ser-295 residue of XIRP1. (Chang et al., 2013).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 100 µL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000

Validation Images & Assay Conditions

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Western Blot (WB) analysis of specific cells using Phospho-Dok-2 (Y299) Polyclonal antibody.

Gene/Protein Information For DOK2 (Source: Uniprot.org, NCBI)

Alternative Names

docking protein 2; docking protein 2, 56kD; docking protein 2, 56kDa; Dok-2; Downstream of tyrosine kinase 2; p56(dok-2); p56DOK; p56dok-2 DOK2 p56DOK, p56dok-2 docking protein 2 docking protein 2|docking protein 2, 56kD|docking protein 2, 56kDa|downstream of tyrosine kinase 2|p56(dok-2)

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