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Product Info Summary
| SKU: | P07956-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Monkey, Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Phospho-Dok-2 (Y299) Antibody
SKU/Catalog Number
P07956-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Phospho-Dok-2 (Y299) Antibody catalog # P07956-1. Tested in WB applications. This antibody reacts with Human, Monkey, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-Dok-2 (Y299) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P07956-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human p56 Dok-2 around the phosphorylation site of Tyr299. AA range:266-315
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
P07956-1 is reactive to DOK2 in Human, Monkey, Mouse
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Observed Molecular Weight
52 kDa
Calculated molecular weight
45379 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P07956-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
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Western Blot analysis of various cells using Phospho-Dok-2 (Y299) polyclonal antibody
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using p56 Dok-2 (Phospho-Tyr299) antibody
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Western blot analysis of lysates from COS7 cells treated with insulin 0.01U/ml 15', Jurkat cells treated with insulin 0.01U/ml 15' and 293 cells treated with serum 20% 15', using p56 Dok-2 (Phospho-Tyr299) antibody. The lane on the right is blocked with the phospho peptide.
Protein Target Info & Infographic
Gene/Protein Information For DOK2 (Source: Uniprot.org, NCBI)
Gene Name
DOK2
Full Name
Docking protein 2
Weight
45379 MW
Superfamily
DOK family
Alternative Names
Docking protein 2;Downstream of tyrosine kinase 2;p56 (dok-2);DOK2; DOK2 p56DOK, p56dok-2 docking protein 2 docking protein 2|docking protein 2, 56kD|docking protein 2, 56kDa|downstream of tyrosine kinase 2|p56(dok-2)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DOK2, check out the DOK2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DOK2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-Dok-2 (Y299) Antibody (P07956-1)
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No publications found for P07956-1
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Customer Q&As
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4 Customer Q&As for Anti-Phospho-Dok-2 (Y299) Antibody
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukemic t-cell using anti-Phospho-Dok-2 (Y299) antibody P07956-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-03-25
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-25
Question
Would anti-Phospho-Dok-2 (Y299) antibody P07956-1 work for WB with leukemic t-cell?
Verified Customer
Verified customer
Asked: 2019-08-05
Answer
According to the expression profile of leukemic t-cell, DOK2 is highly expressed in leukemic t-cell. So, it is likely that anti-Phospho-Dok-2 (Y299) antibody P07956-1 will work for WB with leukemic t-cell.
Boster Scientific Support
Answered: 2019-08-05
Question
Will P07956-1 anti-Phospho-Dok-2 (Y299) antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
J. Parker
Verified customer
Asked: 2019-01-29
Answer
It shows on the product datasheet, P07956-1 anti-Phospho-Dok-2 (Y299) antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-01-29
Question
We are currently using anti-Phospho-Dok-2 (Y299) antibody P07956-1 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, monkey, mouse. Is it possible that the antibody can work on pig tissues as well?
P. Brown
Verified customer
Asked: 2017-02-01
Answer
The anti-Phospho-Dok-2 (Y299) antibody (P07956-1) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-02-01
