Product Info Summary
SKU: | A00195Y420 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | ELISA, IHC |
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Product info
Product Name
Anti-Phospho-AIP4 (Y420) ITCH Antibody
SKU/Catalog Number
A00195Y420
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho-AIP4 (Y420) ITCH Antibody catalog # A00195Y420. Tested in ELISA, IHC applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-AIP4 (Y420) ITCH Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00195Y420)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human ITCH around the phosphorylation site of Tyr420. AA range:386-435
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00195Y420 is reactive to ITCH in Human, Mouse
Reconstitution
Calculated molecular weight
102803 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00195Y420 is guaranteed for ELISA, IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IHC 1:100-1:300
ELISA 1:20000
Validation Images & Assay Conditions
Click image to see more details
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using ITCH (Phospho-Tyr420) antibody
Click image to see more details
Immunohistochemistry analysis of paraffin-embedded human brain, using ITCH (Phospho-Tyr420) antibody. The picture on the right is blocked with the phospho peptide.
Protein Target Info & Infographic
Gene/Protein Information For ITCH (Source: Uniprot.org, NCBI)
Gene Name
ITCH
Full Name
E3 ubiquitin-protein ligase Itchy
Weight
102803 MW
Alternative Names
E3 ubiquitin-protein ligase Itchy homolog;Itch;2.3.2.26 Itch|6720481N21Rik, 8030492O04Rik, A, A130065M08, AIP4, C230047C07Rik|itchy, E3 ubiquitin protein ligase|E3 ubiquitin-protein ligase Itchy|HECT-type E3 ubiquitin transferase Itchy homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ITCH, check out the ITCH Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ITCH: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-AIP4 (Y420) ITCH Antibody (A00195Y420)
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