Human TNF Beta PicoKine™ ELISA Kit

SKU EK0584
Size 96wells/kit, with removable strips.
Reactivity Human
Sample Type cell culture supernates, serum and plasma(heparin, EDTA, citrate).
Sample Volume 100ul per well
Sensitivity <5pg/ml
Assay Range 62.5pg/ml-4000pg/ml

Overview

Product Name Human TNF Beta PicoKine™ ELISA Kit
SKU/Catalog Number EK0584
Storage & Handling Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Size 96wells/kit, with removable strips.
Description Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human TNF beta. 96wells/kit, with removable strips.
Cite This Product Human TNF Beta PicoKine™ ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0584)
Sample Type cell culture supernates, serum and plasma(heparin, EDTA, citrate). . Anticoagulant(s): heparin, EDTA or citrate
*The recommended anticoagulants are proven to not block the antibody binding sites on the target antigen. Please do not collect blood sample with other anticoagulants that are not specified above or contact us to check for feasibility.
Sensitivity <5pg/ml
Assay Range 62.5pg/ml-4000pg/ml
Immunogen Expression system for standard: E.coli; Immunogen sequence: L35-L205
Reactivity Human
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Antibody Clonalities Capture Antibody | Detection Antibody:
monoclonal antibody from mouse|polyclonal antibody from goat

Assay Details

Kit Components

Catalog number Description Quantity
EK0584-CAP 96-well plate precoated with anti-Human LTA antibody 1
EK0584-ST lyophilized recombinant Human LTA standard 10ng/tube
EK0584-DA biotinylated anti-Human LTA antibody 130ul
AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul
AR1106-1 sample diluent buffer 30ml
AR1106-2 antibody diluent buffer 12ml
AR1106-3 ABC diluent buffer 12ml
AR1104 TMB color developing agent 10ml
AR1105 TMB stop solution 10ml
PLA-SEA Adhesive cover 4

*Why there is no wash buffer? Our Avidin-Biotin-Peroxidase Diluent contains the detergent (TWEEN) normally present in other companies' ELISA kits. This saves you the step of having to wash with the special wash buffer and achieve similar or better signal to noise ratio. The wash can use regular wash buffers (PBS, TBS etc.) commonly found in labs.

Materials Required But Not Provided

  • Microplate reader in standard size.
  • Automated plate washer.
  • Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
  • Clean tubes and Eppendorf tubes.
  • Washing buffer (neutral PBS or TBS).
  • Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
  • Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

Typical Data Obtained from Human TNF Beta PicoKine™ ELISA Kit

(TMB reaction incubate at 37°C for 15-25min)

Concentration(pg/ml)062.5125250500100020004000
O.D.0.0380.1800.2930.4500.7371.3451.6681.926

Intra/Inter Assay Precision

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)16548717911795211896
Standard deviation11.7132.14127.1613.0638.55157.36
CV(%)7.1%6.6%7.1%7.3%7.4%8.3%

Reproducibility

Three samples with differing target protein concentrations were assayed using four different lots to measure the CV% lot to lot variance.

Reproducibility

To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

LotsLot1 (pg/ml)Lot2 (pg/ml)Lot3 (pg/ml)Lot4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 11651621541421558.895.7%
Sample 248745445841345326.375.8%
Sample 31791177817601730176422.881.2%
*number of samples for each test n=16.

*The typical data is obtained from Boster's internal QC result and for reference only. It may differ from the lab test results of the end users. It is more important that the user's lab test results reflect the same linearity demonstrated in the typical data than achieving exactly the same O.D. values.

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P01374
Gene Name LTA
Protein Name Lymphotoxin-alpha
Alternative Names Lymphotoxin-alpha;LT-alpha;TNF-beta;Tumor necrosis factor ligand superfamily member 1;LTA;TNFB, TNFSF1;
Subcellular Localization Secreted. Membrane. The homotrimer is secreted. The heterotrimer is membrane-associated.
Molecular Weight 22297 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Cytokine that in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM. In its heterotrimeric form with LTB binds to TNFRSF3/LTBR. Lymphotoxin is produced by lymphocytes and cytotoxic for a wide range of tumor cells in vitro and in vivo.
Research Areas Human

*You can search these to find other products in these research areas.
Background Tumor necrosis factor-beta(TNF-beta), previously called lymphotoxin-alpha(LTA), is a cytokine produced by lymphocytes. TNF-alpha and TNF-beta share 35% identity and 50% homology in the amino acid sequence. The substance LTA mediates a wide variety of inflammatory, immunostimulatory, and antiviral responses. LTA is associated with susceptibility to myocardial infarction, asthma and other diseases. The LTA gene is located on human chromosome 6. The standard product used in this kit is recombinant protein, consisting of 172 amino acids with the molecular mass of 18.8KDa.

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EK0584
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$369.00

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Publications

Effects of Reactive Nitrogen Scavengers on NK-Cell-Mediated Killing of K562 Cells
Wang M, Zheng Z, Meng J, Wang H, He M, Zhang F, Liu Y, Hu B, He Z, Hu Q, Wang H. Biomaterials. 2015 Sep;64:78-87. Doi: 10.1016/J.Biomaterials.2015.06.030. Epub 2015 Jun 20. In Vivo Study Of Immunogenicity And Kinetic Characteristics Of A Quantum D...

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