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Product Info Summary
| SKU: | EK0899 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human THBS1/TSP1/Thrombospondin-1 ELISA Kit PicoKine®
View all Thrombospondin-1 ELISA kits
SKU/Catalog Number
EK0899
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human THBS1/TSP1/Thrombospondin-1 ELISA Kit PicoKine® (96 Tests). Quantitate Human THBS1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human THBS1/TSP1/Thrombospondin-1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0899)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: N19-P1170
Sensitivity
<50 pg/ml
Assay Range
1.56 ng/ml - 100 ng/ml
Standard Dilution Instructions
See datasheet of EK0899 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0899 is reactive to THBS1 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0899 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Thrombospondin-1
Thrombospondin 1, also known as THBS1, is a protein that in humans in encoded by the THBS1 gene. Thrombospondin 1 is a subunit of a disulfide-linked homotrimeric protein. This protein is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. By in situ hybridization, the THBS1 gene was mapped to human 15q15 and the cognate gene to mouse chromosome 2 (region F) and was localized to 15q11-qter by Southern analysis of human-rodent somatic cell hybrids. Thrombospondin I is a multimodular secreted protein that associates with the extracellular matrix and possesses a variety of biologic functions, including a potent antiangiogenic activity. Other thrombospondin genes include thrombospondins II, III, and IV.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0899-CAP | Anti-Human THBS1 Pre-coated 96-well strip microplate | 1 |
| EK0899-ST | Human THBS1 Standard | 2 vials, 100 ng/tube |
| EK0899-DA | Human THBS1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (ng/ml) | 0 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 | 100 |
| O.D. | 0.116 | 0.225 | 0.297 | 0.401 | 0.747 | 1.194 | 1.693 | 2.074 |
Data Example Images
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Human THBS1/TSP1 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Thrombospondin-1, For serum, dilution ratio is 1:1000 and concentration is 7035-34002 ng/ml.
For plasma, dilution ratio is 1:10 and concentration is 13-602 ng/ml..
Some articles we found to cite concentrations of Thrombospondin-1 in samples: 25045452 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human THBS1/TSP1/Thrombospondin-1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 2414 | 13444 | 38908 | 2523 | 13902 | 38494 |
| Standard deviation | 152.08 | 994.85 | 2451.2 | 176.61 | 1278.98 | 2771.56 |
| CV (%) | 9.2%% | 7.4% | 9.2%% | 7% | 9.2% | 7.2% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Thrombospondin-1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 2414 | 2383 | 2098 | 2136 | 2257 | 141.81 | 6.2% |
| Sample 2 | 13444 | 12251 | 11532 | 12803 | 12507 | 703.87 | 5.6% |
| Sample 3 | 38908 | 44108 | 38626 | 45595 | 41809 | 3088.95 | 7.3% |
Protein Target Info & Infographic
Gene/Protein Information For THBS1 (Source: Uniprot.Org, NCBI)
Gene Name
THBS1
Full Name
Thrombospondin-1
Weight
129383 MW
Superfamily
thrombospondin family
Alternative Names
Thrombospondin-1;THBS1;TSP, TSP1; THBS1 THBS, THBS-1, TSP, TSP-1, TSP1 thrombospondin 1 thrombospondin-1|glycoprotein G|thrombospondin-1p180|thrombospondin-p50
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on THBS1, check out the THBS1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for THBS1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human THBS1/TSP1/Thrombospondin-1 ELISA Kit PicoKine® (EK0899)
Hello CJ!
EK0899 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Serum Exosomal Proteins F9 and TSP-1 as Potential Diagnostic Biomarkers for Newly Diagnosed Epilepsy
Maternal serum thrombospondin-1 is significantly altered in cases with established preeclampsia
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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13 Customer Q&As for Human THBS1/TSP1/Thrombospondin-1 ELISA Kit PicoKine®
Question
Can EK0899 detect 6-500aa?
Verified customer
Asked: 2022-06-03
Answer
The Human THBS1/TSP1/Thrombospondin-1 ELISA Kit PicoKine® (EK0899) detects 19-1170aa.
Boster Scientific Support
Answered: 2022-06-06
Question
What is the species of the immunogen of the capture antibody in EK0899, human or something else?
Verified customer
Asked: 2022-06-03
Answer
The species of the immunogen of the capture antibody in the Human THBS1/TSP1/Thrombospondin-1 ELISA Kit PicoKine® (EK0899) is mouse.
Boster Scientific Support
Answered: 2022-06-06
Question
What is the species of the immunogen of the detection antibody in EK0899? Human or something else?
Verified customer
Asked: 2022-06-03
Answer
The species of the immunogen of the capture antibody in the Human THBS1/TSP1/Thrombospondin-1 ELISA Kit PicoKine® (EK0899) is goat.
Boster Scientific Support
Answered: 2022-06-06
Question
What is the standard of EK0899? A recombinant protein or something else? If a recombinant protein, what is the host?
Verified customer
Asked: 2022-06-03
Answer
The standard of the Human THBS1/TSP1/Thrombospondin-1 ELISA Kit PicoKine® (EK0899) is a recombinant protein. The host is NS0, mouse myeloma cell line.
Boster Scientific Support
Answered: 2022-06-06
Question
What is the species of the standard for EK0899? human or something else?
Verified customer
Asked: 2022-06-03
Answer
For the Human THBS1/TSP1/Thrombospondin-1 ELISA Kit PicoKine® (EK0899), the species of the standard is NS0, mouse myeloma cell line.
Boster Scientific Support
Answered: 2022-06-06
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement Thrombospondin-1 using the EK0899 Human Thrombospondin-1 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2020-08-06
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples in order to limit protein degradation.
Boster Scientific Support
Answered: 2020-08-06
Question
Q: What is the optimal O.D. value for Thrombospondin-1 ELISA kit? I used your Thrombospondin-1 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain Thrombospondin-1 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2020-05-11
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Thrombospondin-1 ELISA kit to have sensitivity of 50pg/ml, that means the minimum amount of Thrombospondin-1 that can be declared/interpreted as positive by the above standard is 50pg/ml.
Boster Scientific Support
Answered: 2020-05-11
Question
Can EK0899 be good to detect TSP1 at low level and is it okay to add the cell culture supernant directly to the wells without any dilution?
Verified customer
Asked: 2019-04-26
Answer
If the expression level of TSP1 in the cell culture supernant is low, the customer can add the sample directly to the wells without any dilution. The Human THBS1 / TSP1 / Thrombospondin 1 ELISA Kit PicoKine™ (EK0899) is able to detect the target protein if the concentraion of TSP1 in the sample falls in the assay range of 1.56ng/ml-100ng/ml.
Boster Scientific Support
Answered: 2019-04-26
Question
Q: is it okay to use citrate plasma as samples in Human Thrombospondin-1 Picokine® ELISA Kit (Catalog # EK0899)?
Verified Customer
Verified customer
Asked: 2019-04-01
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of Thrombospondin-1 causing degradation of its protein structure. Thrombospondin-1 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the Thrombospondin-1 ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2019-04-01
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
C. Johnson
Verified customer
Asked: 2018-03-04
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer may affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2018-03-04
Question
Q: we need your recommendation regarding the dilution ratio of serum samples for detection of Thrombospondin-1 in Human serum? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.
A. Garcia
Verified customer
Asked: 2018-02-07
Answer
A: having little idea about the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Thrombospondin-1 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2018-02-07
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Y. Yang
Verified customer
Asked: 2016-09-23
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2016-09-23
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2016-01-01
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2016-01-01
