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Product Info Summary
| SKU: | EK1241 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin). |
Product info
Product Name
Human SPINK1/TATI ELISA Kit PicoKine®
SKU/Catalog Number
EK1241
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human SPINK1/TATI ELISA Kit PicoKine® (96 Tests). Quantitate Human SPINK1 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human SPINK1/TATI ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1241)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: D24-C79
Sensitivity
<10 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1241 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1241 is reactive to SPINK1 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin).
Application Guarantee
EK1241 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of SPINK1
Serine protease inhibitor Kazal-type 1, also called TATI or PSTI, is a protein that in humans is encoded by the SPINK1 gene. It is mapped to 5q32. SPINK1 gene contains 4 exons spanning approximately 7.5 kb. It was initially detected in urine of patients with ovarian cancer. This peptide is also produced by the mucosa of the gastrointestinal tract where it may protect the mucosal cells from proteolytic breakdown. The SPINK1 gene encodes pancreatic secretory trypsin inhibitor, which is secreted from pancreatic acinar cells into pancreatic juice. Its physiologic role was thought to be the prevention of trypsin-catalyzed premature activation of zymogens within the pancreas and the pancreatic duct. Mutations in this gene are associated with hereditary pancreatitis and tropical calcific pancreatitis.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1241-CAP | Anti-Human SPINK1 Pre-coated 96-well strip microplate | 1 |
| EK1241-ST | Human SPINK1 Standard | 2 vials, 10 ng/tube |
| EK1241-DA | Human SPINK1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.068 | 0.101 | 0.149 | 0.260 | 0.384 | 0.867 | 1.411 | 1.892 |
Data Example Images
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Human SPINK1/TATI PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect SPINK1, Dilution ratio of 1:1, concentration in serum and plasma is around 2 ng/ml..
Some articles we found to cite concentrations of SPINK1 in samples: 24254562 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human SPINK1/TATI ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 245 | 1862 | 5817 | 254 | 1784 | 6136 |
| Standard deviation | 16.9 | 137.22 | 418.82 | 17.78 | 148.07 | 468.33 |
| CV (%) | 6.9% | 7.3% | 7.2% | 7% | 8.3% | 7.6% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of SPINK1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 245 | 238 | 239 | 225 | 236 | 7.29 | 3% |
| Sample 2 | 1862 | 1592 | 1831 | 1832 | 1779 | 108.82 | 6.1% |
| Sample 3 | 5817 | 5596 | 5823 | 5918 | 5788 | 118.14 | 2% |
Protein Target Info & Infographic
Gene/Protein Information For SPINK1 (Source: Uniprot.Org, NCBI)
Gene Name
SPINK1
Full Name
Serine protease inhibitor Kazal-type 1
Weight
8507 MW
Alternative Names
Pancreatic secretory trypsin inhibitor;Serine protease inhibitor Kazal-type 1;Tumor-associated trypsin inhibitor;TATI;SPINK1;PSTI; SPINK1 PCTT, PSTI, Spink3, TATI, TCP serine peptidase inhibitor Kazal type 1 serine protease inhibitor Kazal-type 1|pancreatic secretory trypsin inhibitor|serine protease inhibitor, Kazal type 1|tumor-associated trypsin inhibitor
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on SPINK1, check out the SPINK1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for SPINK1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human SPINK1/TATI ELISA Kit PicoKine® (EK1241)
Hello CJ!
EK1241 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Serine protease inhibitor Kazal type 1 (SPINK1) drives proliferation and anoikis resistance in a subset of ovarian cancers
Targeting an autocrine IL-6–SPINK1 signaling axis to suppress metastatic spread in ovarian clear cell carcinoma
Serine protease inhibitor Kazal type 1 (SPINK1) drives proliferation and anoikis resistance in a subset of ovarian cancers
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Human SPINK1/TATI ELISA Kit PicoKine®
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
C. Hill
Verified customer
Asked: 2020-10-10
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2020-10-10
Question
Q: how can I thaw whole blood sample for SPINK1 ELISA after freezing?
P. King
Verified customer
Asked: 2019-12-12
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test SPINK1 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2019-12-12
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
S. Brown
Verified customer
Asked: 2019-02-22
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2019-02-22
Question
Q: can I use citrate plasma as samples in Human SPINK1 Picokine® ELISA Kit (Catalog # EK1241)?
W. Baker
Verified customer
Asked: 2019-02-13
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of SPINK1 causing disruption of its protein structure. SPINK1 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the SPINK1 ELISA, treating samples with various anticoagulants and decided that heparin can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2019-02-13
Question
Q: we need your recommendation regarding the dilution ratio of serum samples for detection of SPINK1 in Human plasma? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2018-10-31
Answer
A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the SPINK1 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2018-10-31
Question
Q: What is the optimal O.D. value for SPINK1 ELISA kit? I performed your SPINK1 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain SPINK1 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2018-01-16
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this SPINK1 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of SPINK1 that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2018-01-16
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification SPINK1 using the EK1241 Human SPINK1 Picokine® ELISA Kit?
L. Thompson
Verified customer
Asked: 2016-01-26
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to avoid protein degradation.
Boster Scientific Support
Answered: 2016-01-26
