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Product Info Summary
| SKU: | PB9046 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Cathepsin B/CTSB Antibody Picoband®
View all Cathepsin B Antibodies
SKU/Catalog Number
PB9046
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Cathepsin B/CTSB Antibody Picoband® catalog # PB9046. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cathepsin B/CTSB Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9046)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Cathepsin B recombinant protein (Position: L80-D333). Human Cathepsin B shares 83% and 84% amino acid (aa) sequences identity with mouse and rat Cathepsin B, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9046 is reactive to CTSB in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
33 kDa
Calculated molecular weight
37822 MW
Background of Cathepsin B
Cathepsin B is an enzymatic protein belonging to the peptidase or protease families. In humans, it is coded by the CTSB gene. And this gene is mapped to chromosome 8p22. The protein encoded by this gene is a lysosomal cysteine proteinase composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. It is a member of the peptidase C1 family. Cathepsin B was once suspected as a candidate protease participating in the conversion of beta-amyloid precursor protein into the amyloid plaques found in Alzheimer's disease patients. However, this function is now known to be mediated by BACE1 protease. It is now thought that cathepsin B can degrade beta-amyloid precursor protein into harmless fragments. Thus, it is conceivable cathepsin B may play a pivotal role in the natural defense against Alzheimer's disease. Overexpression of cathepsin B has been associated with esophageal adenocarcinoma and other tumors. At least five transcript variants encoding the same protein have been found for this gene. The standard product used in this kit is recombinant human Cathepsin B with the molecular mass of 37KDa.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9046 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: human U87 whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Cathepsin B using anti-Cathepsin B antibody (PB9046).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 5 ng of sample under reducing conditions.
Lane 1: human U87 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cathepsin B antigen affinity purified polyclonal antibody (Catalog # PB9046) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cathepsin B at approximately 33 kDa. The expected band size for Cathepsin B is at 38 kDa.
Protein Target Info & Infographic
Gene/Protein Information For CTSB (Source: Uniprot.org, NCBI)
Gene Name
CTSB
Full Name
Cathepsin B
Weight
37822 MW
Superfamily
peptidase C1 family
Alternative Names
Cathepsin B;3.4.22.1;APP secretase;APPS;Cathepsin B1;Cathepsin B light chain;Cathepsin B heavy chain;CTSB;CPSB; CTSB APPS, CPSB, RECEUP cathepsin B cathepsin B|APP secretase|amyloid precursor protein secretase|cathepsin B1|cysteine protease|epididymis secretory sperm binding protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CTSB, check out the CTSB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CTSB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cathepsin B/CTSB Antibody Picoband® (PB9046)
Hello CJ!
PB9046 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Inhibition of autophagosome-lysosome fusion by ginsenoside Ro via the ESR2-NCF1-ROS pathway sensitizes esophageal cancer cells to 5-fluorouracil-induced cell death via the CHEK1-mediated DNA damage checkpoint
Zhang Zy, Mai Y, Yang H, Dong Py, Zheng Xl, Yang Gs. Mol Cell Biochem. 2014 Oct;395(1-2):53-64. Doi: 10.1007/S11010-014-2111-6. Epub 2014 May 31. Ctsb Promotes Porcine Preadipocytes Differentiation By Degrading Fibronectin And Attenuating The Wnt/...
Yang D, L?? X, Hong Y, Xi T, Zhang D. Biomaterials. 2014 Aug;35(24):6195-205. Doi: 10.1016/J.Biomaterials.2014.04.069. Epub 2014 May 10. The Molecular Mechanism For Effects Of Tin Coating On Niti Alloy On Endothelial Cell Function.
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Customer Q&As
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15 Customer Q&As for Anti-Cathepsin B/CTSB Antibody Picoband®
Question
My boss were happy with the WB result of your anti-Cathepsin B/CTSB antibody. However we have seen positive staining in brain placenta lysosome. using this antibody. Is that expected? Could you tell me where is CTSB supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-04-22
Answer
From literature, brain placenta does express CTSB. Generally CTSB expresses in lysosome. Regarding which tissues have CTSB expression, here are a few articles citing expression in various tissues:
Brain, and Placenta, Pubmed ID: 15489334
Esophagus, Pubmed ID: 14702039
Gastric carcinoma, Pubmed ID: 8112600
Liver, Pubmed ID: 1637335, 3972105, 24275569
Melanoma, Pubmed ID: 12643545, 17081065
Boster Scientific Support
Answered: 2020-04-22
Question
Is a blocking peptide available for product anti-Cathepsin B/CTSB antibody (PB9046)?
Verified Customer
Verified customer
Asked: 2020-04-01
Answer
We do provide the blocking peptide for product anti-Cathepsin B/CTSB antibody (PB9046). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-04-01
Question
I was wanting to use your anti-Cathepsin B/CTSB antibody for WB for human esophagus on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human esophagus identification?
Verified Customer
Verified customer
Asked: 2019-12-10
Answer
You can see on the product datasheet, PB9046 anti-Cathepsin B/CTSB antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human esophagus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-10
Question
We want to test anti-Cathepsin B/CTSB antibody PB9046 on human esophagus for research purposes, then I may be interested in using anti-Cathepsin B/CTSB antibody PB9046 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
P. Thomas
Verified customer
Asked: 2019-12-04
Answer
The products we sell, including anti-Cathepsin B/CTSB antibody PB9046, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-12-04
Question
Is this PB9046 anti-Cathepsin B/CTSB antibody reactive to the isotypes of CTSB?
Verified Customer
Verified customer
Asked: 2019-11-08
Answer
The immunogen of PB9046 anti-Cathepsin B/CTSB antibody is E.coli-derived human Cathepsin B recombinant protein (Position: L80-D333). Human Cathepsin B shares 83% and 84% amino acid (aa) sequences identity with mouse and rat Cathepsin B, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-11-08
Question
Do you have a BSA free version of anti-Cathepsin B/CTSB antibody PB9046 available?
Verified Customer
Verified customer
Asked: 2019-10-25
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Cathepsin B/CTSB antibody PB9046 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-10-25
Question
Does anti-Cathepsin B/CTSB antibody PB9046 work for WB with esophagus?
Verified Customer
Verified customer
Asked: 2019-07-02
Answer
According to the expression profile of esophagus, CTSB is highly expressed in esophagus. So, it is likely that anti-Cathepsin B/CTSB antibody PB9046 will work for WB with esophagus.
Boster Scientific Support
Answered: 2019-07-02
Question
We are currently using anti-Cathepsin B/CTSB antibody PB9046 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-04-16
Answer
The anti-Cathepsin B/CTSB antibody (PB9046) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-04-16
Question
I have a question about product PB9046, anti-Cathepsin B/CTSB antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-11-14
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9046 anti-Cathepsin B/CTSB antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-11-14
Question
Would PB9046 anti-Cathepsin B/CTSB antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-10-16
Answer
It shows on the product datasheet, PB9046 anti-Cathepsin B/CTSB antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-10-16
Question
I am looking for using your anti-Cathepsin B/CTSB antibody for decidualization studies. Has this antibody been tested with western blotting on hepg2 whole cell lysate? We would like to see some validation images before ordering.
B. Jones
Verified customer
Asked: 2018-08-30
Answer
Thanks for your inquiry. This PB9046 anti-Cathepsin B/CTSB antibody is validated on hepg2 whole cell lysate. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-08-30
Question
We have been able to see staining in human brain placenta. What should we do? Is anti-Cathepsin B/CTSB antibody supposed to stain brain placenta positively?
Verified Customer
Verified customer
Asked: 2018-05-25
Answer
Based on literature brain placenta does express CTSB. Based on Uniprot.org, CTSB is expressed in thyroid gland, gastric carcinoma, esophagus, brain placenta, liver, melanoma, among other tissues. Regarding which tissues have CTSB expression, here are a few articles citing expression in various tissues:
Brain, and Placenta, Pubmed ID: 15489334
Esophagus, Pubmed ID: 14702039
Gastric carcinoma, Pubmed ID: 8112600
Liver, Pubmed ID: 1637335, 3972105, 24275569
Melanoma, Pubmed ID: 12643545, 17081065
Boster Scientific Support
Answered: 2018-05-25
Question
See attached the WB image, lot number and protocol we used for esophagus using anti-Cathepsin B/CTSB antibody PB9046. Please let me know if you require anything else.
K. Johnson
Verified customer
Asked: 2017-12-15
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-12-15
Question
I see that the anti-Cathepsin B/CTSB antibody PB9046 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-10-04
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-10-04
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for esophagus using anti-Cathepsin B/CTSB antibody PB9046. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-07-07
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-07-07
