Product Info Summary
SKU: | PROTP36222-4 |
---|---|
Size: | 5ug,20ug,100ug |
Origin Species: | Human |
Source: | Escherichia coli |
Application: | Cell Culture |
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Product info
Product Name
Human recombinant CHI3L1 (Chitinase-3-like protein 1) protein, AF
View all Chitinase 3-like 1 recombinant proteins
SKU/Catalog Number
PROTP36222-4
Size
5ug,20ug,100ug
Tag
His Tag (C-term)
Description
Chitinases and non-enzymatic chitinase-like proteins (CLPs, can bind chitin but cannot digest) belong to Glycoside hydrolase family 18. Human CHI3L1, YKL-40, is a CLP and a 42 kDa protein with 383 amino acid residues. The complex (CHI3L1, IL-13R alpha2 and IL-13) regulate downstream signal transduction pathway related to inflammation, proliferation and metastasis.
Storage & Handling
Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
Cite This Product
Human recombinant CHI3L1 (Chitinase-3-like protein 1) protein, AF (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTP36222-4)
Form
Lyophilized
Formulation
The protein was lyophilized from a 0.2 μm filtered solution containing 1X PBS, pH 7.4. If you have any concerns or special requirements, please confirm with us.
Purity
>98% as determined by SDS-PAGE.
Predicted MW
42.625kDa
Molecular weight
The protein has a calculated MW of 41.43 kDa. The protein migrates as 40 kDa under reducing condition (SDS-PAGE analysis).
Activity
Testing in process
Endotoxin
<0.1 EU per 1 μg of the protein by the LAL method.
Amino Acid Sequence
MYKLVCYYTSWSQYREGDGSCFPDALDRFLCTHIIYSFANISNDHIDTWEWNDVTLYGMLNTLKNRNPNLKTLLSVGGWNFGSQRFSKIASNTQSRRTFIKSVPPFLRTHGFDGLDLAWLYPGRRDKQHFTTLIKEMKAEFIKEAQPGKKQLLLSAALSAGKVTIDSSYDIAKISQHLDFISIMTYDFHGAWRGTTGHHSPLFRGQEDASPDRFSNTDYAVGYMLRLGAPASKLVMGIPTFGRSFTLASSETGVGAPISGPGIPGRFTKEAGTLAYYEICDFLRGATVHRILGQQVPYATKGNQWVGYDDQESVKSKVQYLKDRQLAGAMVWALDLDDFQGSFCGQDLRFPLTNAIKDALAAT with polyhistidine tag at the C-terminus.
Reconstitution
Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
Assay dilution & Images
Validation Images & Assay Conditions
Click image to see more details
SDS- PAGE analysis of recombinant human CHI3L1
Protein Target Info & Infographic
Gene/Protein Information For CHI3L1 (Source: Uniprot.org, NCBI)
Gene Name
CHI3L1
Full Name
Chitinase-3-like protein 1
Weight
42.625kDa
Superfamily
glycosyl hydrolase 18 family
Alternative Names
ASRT7, CGP-39, GP-39, GP39, HC-gp39, HCGP-3P, YK-40, YKL-40, YKL40, YYL-40, hCGP-39 CHI3L1 ASRT7, CGP-39, GP-39, GP39, HC-gp39, HCGP-3P, YK-40, YKL-40, YKL40, YYL-40, hCGP-39 chitinase 3 like 1 chitinase-3-like protein 1|39 kDa synovial protein|cartilage glycoprotein 39|chitinase 3-like 1 (cartilage glycoprotein-39)
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on CHI3L1, check out the CHI3L1 Infographic
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In this infographic, you will see the following information for CHI3L1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us at [email protected].
Specific Publications For Human recombinant CHI3L1 (Chitinase-3-like protein 1) protein, AF (PROTP36222-4)
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