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Product Info Summary
| SKU: | EK1873 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin or EDTA) |
Product info
Product Name
Human Pregnancy Zone Protein/PZP ELISA Kit PicoKine®
View all Pregnancy Zone Protein ELISA kits
SKU/Catalog Number
EK1873
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human PZP ELISA Kit PicoKine® (96 Tests). Quantitate Human PZP in cell culture supernatants, serum and plasma (heparin or EDTA) . Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Pregnancy Zone Protein/PZP ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1873)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: T26-V1482
Sensitivity
<50 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1873 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1873 is reactive to PZP in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin or EDTA)
Application Guarantee
EK1873 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Pregnancy Zone Protein
Pregnancy zone protein (PZP) is a protein which in humans is encoded by the PZP gene on chromosome 12. It is mapped to 12p13.31. The protein encoded by this gene is highly expressed in late-pregnancy serum and is similar in structure to alpha-2-macroglobulin. The encoded protein, which acts as a homotetramer, inhibits the activity of all four classes of proteinases. This protein contains cleavage sites for several proteinases. Upon binding of a proteinase, the conformation of this protein changes to trap the proteinase, limiting its activity. This protein appears to be elevated in the sera of presymptomatic Alzheimer's disease patients.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1873-CAP | Anti-Human PZP Pre-coated 96-well strip microplate | 1 |
| EK1873-ST | Human PZP Standard | 2 vials, 10 ng/tube |
| EK1873-DA | Human PZP Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.070 | 0.135 | 0.196 | 0.271 | 0.534 | 0.937 | 1.592 | 2.033 |
Data Example Images
Click image to see more details
Human PZP PicoKine ELISA Kit Standard Curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Pregnancy Zone Protein, Dilution ratio of 1:10000, concentration in serum and plasma is around 20 ug/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Pregnancy Zone Protein/PZP ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 240 | 952 | 4494 | 289 | 1147 | 4732 |
| Standard deviation | 10.08 | 58.07 | 292.11 | 15.61 | 72.26 | 359.63 |
| CV (%) | 4.2% | 6.1% | 6.5% | 5.4% | 6.3% | 7.6% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Pregnancy Zone Protein in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 240 | 252 | 219 | 220 | 232 | 13.91 | 5.9% |
| Sample 2 | 952 | 1062 | 957 | 1054 | 1006 | 51.85 | 5.1% |
| Sample 3 | 4494 | 4533 | 4059 | 4186 | 4318 | 201.06 | 4.6% |
Protein Target Info & Infographic
Gene/Protein Information For PZP (Source: Uniprot.Org, NCBI)
Gene Name
PZP
Full Name
Pregnancy zone protein
Weight
163.863kDa
Superfamily
protease inhibitor I39 (alpha-2-macroglobulin) family
Alternative Names
Pregnancy zone protein; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 6; PZP; CPAMD6 PZP CPAMD6 PZP alpha-2-macroglobulin like pregnancy zone protein|C3 and PZP-like alpha-2-macroglobulin domain-containing protein 6|Pregnancy zone protein|pregnancy-zone protein
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on PZP, check out the PZP Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for PZP: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Pregnancy Zone Protein/PZP ELISA Kit PicoKine® (EK1873)
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No publications found for EK1873
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Human Pregnancy Zone Protein/PZP ELISA Kit PicoKine®
Question
Q: Can Pregnancy Zone Protein ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
D. Davis
Verified customer
Asked: 2020-10-27
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is a must. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2020-10-27
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
K. Williams
Verified customer
Asked: 2020-04-07
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2020-04-07
Question
Q: can I use citrate plasma as samples in Human Pregnancy Zone Protein Picokine® ELISA Kit (Catalog # EK1873)?
Verified Customer
Verified customer
Asked: 2019-06-23
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of Pregnancy Zone Protein causing degradation of its protein structure. Pregnancy Zone Protein may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the Pregnancy Zone Protein ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2019-06-23
Question
Q: if the enzyme conjugated Pregnancy Zone Protein antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Pregnancy Zone Protein antigen?
S. Young
Verified customer
Asked: 2018-10-05
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2018-10-05
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2018-03-09
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2018-03-09
Question
Q: can you recommend the dilution ratio of serum samples for detection of Pregnancy Zone Protein in Human serum? I am trying to measure a a number of analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.
K. Surve
Verified customer
Asked: 2014-06-30
Answer
A: unable to understand the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Pregnancy Zone Protein ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2014-06-30
