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Product Info Summary
| SKU: | A02062-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-IL17F Antibody Picoband®
SKU/Catalog Number
A02062-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-IL17F Antibody Picoband® catalog # A02062-3. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IL17F Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02062-3)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived rat IL17F recombinant protein (Position: R29-A161).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02062-3 is reactive to Il17f in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
30 kDa
Calculated molecular weight
18.045kDa
Background of IL-17F
Interleukin 17F, also called IL17F is involved in the regulation of normal versus aberrant T-cell responses. This gene is mapped to 6p12.2. The protein encoded by this gene is a cytokine that shares sequence similarity with IL17. This cytokine is expressed by activated T cells, and has been shown to stimulate the production of several other cytokines, including IL6, IL8, and CSF2/GM_CSF. This cytokine is also found to inhibit the angiogenesis of endothelial cells and induce endothelial cells to produce IL2, TGFB1/TGFB, and monocyte chemoattractant protein-1. It is suggested that targeting IL17 and IL17F or antagonizing IL17R might mitigate neutrophil-mediated inflammation in CF.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02062-3 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: rat spleen tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of IL17F using anti-IL17F antibody (A02062-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat spleen tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL17F antigen affinity purified polyclonal antibody (Catalog # A02062-3) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL17F at approximately 30KD. The expected band size for IL17F is at 18KD.
Protein Target Info & Infographic
Gene/Protein Information For Il17f (Source: Uniprot.org, NCBI)
Gene Name
Il17f
Full Name
Interleukin-17F
Weight
18.045kDa
Superfamily
IL-17 family
Alternative Names
Interleukin-17F; IL-17F; Il17f; IL17F CANDF6, IL-17F, ML-1, ML1 interleukin 17F interleukin-17F|cytokine ML-1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Il17f, check out the Il17f Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Il17f: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IL17F Antibody Picoband® (A02062-3)
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Customer Q&As
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1 Customer Q&As for Anti-IL17F Antibody Picoband®
Question
We are currently using anti-IL17F antibody A02062-3 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it possible that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-09
Answer
The anti-IL17F antibody (A02062-3) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-09
