Click image to see more details
Product Info Summary
| SKU: | A07282 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-PBK1 RSL1D1 Antibody
SKU/Catalog Number
A07282
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-PBK1 RSL1D1 Antibody (Catalog # A07282). Tested in ELISA, IF, WB applications. This antibody reacts with Human.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-PBK1 RSL1D1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07282)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal sequence of human PBK1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A07282 is reactive to RSL1D1 in Human
Reconstitution
Observed Molecular Weight
42 kDa
Calculated molecular weight
119170 MW
Background of RSL1D1
PBK1 protein (also known as Ribosomal L1 domain-containing protein 1, cellular senescence-inhibited gene protein, and CATX-11) was isolated from highly invasive first trimester trophoblast cells and has been proposed to regulate their naturally occurring invasive behavior (Huch et al., 1998). PBK1 was also found to be over-expressed in non-small-cell lung cancer (NSCLC) cells (Petroziello et al., 2004). A recent study suggests that PBK1 may up-regulate the urokinase-type plasminogen activator (uPA) gene, which plays an important role in cellular matrix degradation and activation of other protease systems involved in cell invasion (Tong et al., 2005). The cellular localization dynamics of PBK1, as well as the data from its yeast homologue (Cic1p/Nsa3p) suggest that PBK1 is essential for ribosome biogenesis (Fatica et al., 2003). Thus, PBK1 could be controlling cell proliferation by regulating the level of ribosome production. It is hypothesized that PBK1 is involved in regulating both, cell proliferation and invasiveness, therefore playing a dual function in cancer cells transformation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07282 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:2,500 - 1:10,000
IF Microscopy: 1:100
IP: 1:100
WB: 1:500 - 1:2,000
Validation Images & Assay Conditions
Click image to see more details
Immunofluorescence microscopy of HeLa cells transfected with GFP-PBK1.In the overlay, specific antibody staining is shown to co-localize with recombinant protein.Cells were fixed with methanol prior to staining.Personal communication, J. McNally and D. Stavreva, NCI, Bethesda, MD.
Click image to see more details
Figure 2. Western blot analysis of RSL1D1 using anti-RSL1D1 antibody (A07282).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RSL1D1 antigen affinity purified polyclonal antibody (Catalog # A07282) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for RSL1D1.
Protein Target Info & Infographic
Gene/Protein Information For RSL1D1 (Source: Uniprot.org, NCBI)
Gene Name
RSL1D1
Full Name
Ribosomal L1 domain-containing protein 1
Weight
119170 MW
Superfamily
universal ribosomal protein uL1 family
Alternative Names
PBK1, PBK-1, PBK 1, CATX-11, CATX11, RSL1D1, Cellular senescence inhibited gene protein, CSIG, L12, Ribosomal L1 domain containing protein 1 RSL1D1 CSIG, L12, PBK1, UTP30 ribosomal L1 domain containing 1 ribosomal L1 domain-containing protein 1|CATX-11|cellular senescence-inhibited gene protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RSL1D1, check out the RSL1D1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RSL1D1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PBK1 RSL1D1 Antibody (A07282)
Hello CJ!
No publications found for A07282
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-PBK1 RSL1D1 Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-PBK1 RSL1D1 Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
4 Customer Q&As for Anti-PBK1 RSL1D1 Antibody
Question
Is there a BSA free version of anti-PBK1 antibody A07282 available?
Verified Customer
Verified customer
Asked: 2020-04-02
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-PBK1 antibody A07282 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-04-02
Question
Will anti-PBK1 antibody A07282 work for WB with brain?
Verified Customer
Verified customer
Asked: 2019-02-06
Answer
According to the expression profile of brain, RSL1D1 is highly expressed in brain. So, it is likely that anti-PBK1 antibody A07282 will work for WB with brain.
Boster Scientific Support
Answered: 2019-02-06
Question
We are currently using anti-PBK1 antibody A07282 for human tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on canine tissues as well?
G. Jackson
Verified customer
Asked: 2016-12-20
Answer
The anti-PBK1 antibody (A07282) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-12-20
Question
Is this A07282 anti-PBK1 antibody reactive to the isotypes of RSL1D1?
R. Wu
Verified customer
Asked: 2016-07-11
Answer
The immunogen of A07282 anti-PBK1 antibody is This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal sequence of human PBK1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2016-07-11
