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Product Info Summary
| SKU: | EK1700 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human PDGF-DD ELISA Kit PicoKine®
View all PDGF-D/SCDGFB ELISA kits
SKU/Catalog Number
EK1700
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human PDGF-DD ELISA Kit PicoKine® (96 Tests). Quantitate Human PDGFD in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml . The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human PDGF-DD ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1700)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: S250-R370
Sensitivity
<10 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1700 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1700 is reactive to PDGFD in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
EK1700 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of PDGF-D/SCDGFB
Platelet-derived growth factor (PDGF) is one of numerous growth factors that regulate cell growth and division.The PDGF family consists of PDGF-A, -B, -C and -D, which form either homo- or heterodimers (PDGF-AA, -AB, -BB, -CC, -DD). The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a core motif of eight cysteines, seven of which are found in this factor. This gene product only forms homodimers and, therefore, does not dimerize with the other three family members. It differs from alpha and beta members of this family in having an unusual N-terminal domain, the CUB domain. Two splice variants have been identified for this gene.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1700-CAP | Anti-Human PDGFD Pre-coated 96-well strip microplate | 1 |
| EK1700-ST | Human PDGFD Standard | 2 vials, 10 ng/tube |
| EK1700-DA | Human PDGFD Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.032 | 0.069 | 0.122 | 0.175 | 0.276 | 0.520 | 1.052 | 1.908 |
Data Example Images
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Human PDGF-DD PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect PDGF-D/SCDGFB, For serum, dilution ratio is 1:5 and concentration is around 2 ng/ml.
For plasma, dilution ratio is 1:1 and concentration is 0-220 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human PDGF-DD ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 51 | 222 | 762 | 57 | 229 | 841 |
| Standard deviation | 2.81 | 9.1 | 43.4 | 3.53 | 14.89 | 61.39 |
| CV (%) | 5.5% | 4.1% | 5.7% | 6.2% | 6.5% | 7.3% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of PDGF-D/SCDGFB in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 51 | 49 | 50 | 50 | 50 | 0.7 | 1.4% |
| Sample . 2 | 222 | 183 | 220 | 190 | 203 | 17.44 | 8.5%% |
| Sample 3 | 762 | 921 | 866 | 889 | 859 | 59.58 | 6.9% |
Protein Target Info & Infographic
Gene/Protein Information For PDGFD (Source: Uniprot.Org, NCBI)
Gene Name
PDGFD
Full Name
Platelet-derived growth factor D
Weight
42.809kDa
Superfamily
PDGF/VEGF growth factor family
Alternative Names
Platelet-derived growth factor D;PDGF-D;Iris-expressed growth factor;Spinal cord-derived growth factor B;SCDGF-B;PDGFD latent form; PDGFD receptor-binding form;IEGF;SCDGFB;MSTP036; UNQ1899/PRO4345 Pdgfd|Scdgfb, rSCDGF-B|platelet derived growth factor D|platelet-derived growth factor D|iris-expressed growth factor|platelet-derived growth factor, D polypeptide|spinal cord-derived growth factor B|spinal-cord derived growth factor-B protein
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on PDGFD, check out the PDGFD Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for PDGFD: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human PDGF-DD ELISA Kit PicoKine® (EK1700)
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Customer Q&As
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7 Customer Q&As for Human PDGF-DD ELISA Kit PicoKine®
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
H. Parker
Verified customer
Asked: 2019-12-15
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-12-15
Question
Q: What is the optimal O.D. value for PDGF-D ELISA kit? I used your PDGF-D ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain PDGF-D even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2018-06-28
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the above example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this PDGF-D ELISA kit to have sensitivity of 10pg/ml , that means the minimum amount of PDGF-D that can be declared/interpreted as positive by the above standard is 10pg/ml .
Boster Scientific Support
Answered: 2018-06-28
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification PDGF-D using the EK1700 Human PDGF-D Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-01-02
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to limit protein degradation.
Boster Scientific Support
Answered: 2018-01-02
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
W. Williams
Verified customer
Asked: 2017-11-04
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2017-11-04
Question
Q: if the enzyme conjugated PDGF-D antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the PDGF-D antigen?
Verified Customer
Verified customer
Asked: 2017-10-09
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2017-10-09
Question
Q: we need your suggestion regarding the dilution ratio of serum samples for detection of PDGF-D in Human plasma? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2017-06-28
Answer
A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the PDGF-D ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2017-06-28
Question
Q: how do I analyze ELISA data? I measured PDGF-D level in plasma.
Verified Customer
Verified customer
Asked: 2014-02-18
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2014-02-18
