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Product Info Summary
| SKU: | PA2224 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Calponin/CNN1 Antibody Picoband®
View all Calponin 1 Antibodies
SKU/Catalog Number
PA2224
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Calponin/CNN1 Antibody catalog # PA2224. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Calponin/CNN1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2224)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Calponin, different from the related mouse and rat sequences by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2224 is reactive to CNN1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
33 kDa
Calculated molecular weight
33170 MW
Background of Calponin 1
Calponin is a basic 34-kD protein first isolated from chicken gizzard and bovine aorta. This gene is mapped to 19p13.2. It is specifically expressed in smooth muscle and binds calmodulin, actin, and tropomyosin. It is able to inhibit the ATPase activity of myosin and is though to play a role in smooth muscle contraction. Calponin is a smooth muscle specific, actin-, tropomyosin- and calmodulin-binding protein though to be involved in some way in the regulation or modulation of contraction.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2224 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: HELA Whole Cell, JURKAT Whole Cell, MCF-7 Whole Cell
IHC: Human Placenta Tissue, mouse spleen tissue, rat spleen tissue
Validation Images & Assay Conditions
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Anti-Calponin antibody, PA2224, IHC(P)
IHC(P): Human Placenta Tissue
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Anti-Calponin antibody, PA2224,Western blotting
All lanes: Anti -Calponin (PA2224) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: JURKAT Whole Cell Lysate at 40ug
Lane 3: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 33KD
Observed bind size: 33KD
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Figure 3. IHC analysis of Calponin using anti-Calponin antibody (PA2224).
Calponin was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Calponin Antibody (PA2224) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Figure 4. IHC analysis of Calponin using anti-Calponin antibody (PA2224).
Calponin was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Calponin Antibody (PA2224) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For CNN1 (Source: Uniprot.org, NCBI)
Gene Name
CNN1
Full Name
Calponin-1
Weight
33170 MW
Superfamily
calponin family
Alternative Names
Calponin-1;Basic calponin;Calponin H1, smooth muscle;CNN1; CNN1 HEL-S-14, SMCC, Sm-Calp calponin 1 calponin-1|basic calponin|calponin 1, basic, smooth muscle|calponin H1, smooth muscle|calponins, basic|epididymis secretory protein Li 14
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CNN1, check out the CNN1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CNN1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Calponin/CNN1 Antibody Picoband® (PA2224)
Hello CJ!
PA2224 has been cited in 7 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Li X,Hu B,Wang L,Xia Q,Ni X. P2X7 receptor-mediated phenotype switching of pulmonary artery smooth muscle cells in hypoxia. Mol Biol Rep.2021 Mar 1.doi:10.1007/s11033-021-06222-2. Epub ahead of print.PMID:33650080.
Species: Rat
PA2224 usage in article: APP:WB, SAMPLE:PASMCS, DILUTION:1:500
Zhao Y,Tang N,Xi D,Huang Z,Zhang T,Liu Y,Wang L,Tang Y,Zhong H,He F.Calcilytic NPS2143 promotes proliferation and inhibits apoptosis of spontaneously hypertensive rat vascular smooth muscle cells via activation of the renin-angiotensin system.Exp Ther Med
Species: Rat
PA2224 usage in article: APP:WB, SAMPLE:VSMCS, DILUTION:1:400
Galectin-3- Mediated Transdifferentiation of Pulmonary Artery Endothelial Cells Contributes to Hypoxic Pulmonary Vascular Remodeling
Functional and Proteomic Investigations Reveal Major Royal Jelly Protein 1 Associated with Anti-hypertension Activity in Mouse Vascular Smooth MuscleCells
Reduced Expression of the Extracellular Calcium-Sensing Receptor (CaSR) Is Associated with Activation of the Renin-Angiotensin System (RAS) to Promote Vascular Remodeling in the Pathogenesis of Essential Hypertension
Effects of BMSCs interactions with adventitial fibroblasts in transdifferentiation and ultrastructure processes
Liu C, Chen L, Zeng J, Cui J, Ning Jn, Wang Gs, Belguise K, Wang X, Qian Gs, Lu Kz, Yi B. Exp Cell Res. 2015 Aug 1;336(1):109-18. Doi: 10.1016/J.Yexcr.2015.05.025. Epub 2015 Jun 10. Bone Morphogenic Protein-2 Regulates The Myogenic Differentiation...
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Customer Q&As
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1 Customer Q&As for Anti-Calponin/CNN1 Antibody Picoband®
Question
We are currently using anti-Calponin/CNN1 antibody PA2224 for mouse tissue, and we are satisfied with the IHC-P results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-04-05
Answer
The anti-Calponin/CNN1 antibody (PA2224) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-04-05
