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Product Info Summary
| SKU: | EK0396 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants and serum. |
Product info
Product Name
Human IL1R2/Il 1 Rii ELISA Kit PicoKine®
SKU/Catalog Number
EK0396
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human IL1R2/Il 1 Rii ELISA Kit PicoKine® (96 Tests). Quantitate Human IL1R2 in cell culture supernatants and serum. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human IL1R2/Il 1 Rii ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0396)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: F14-E343
Sensitivity
<10 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0396 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0396 is reactive to IL1R2 in Human samples
Validated Sample Types
cell culture supernatants and serum.
Application Guarantee
EK0396 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of IL-1 RII
Interleukin 1 receptor, type II (IL1R2) also known as CD121b (Cluster of Differentiation 121b) is an interleukin receptor. The protein encoded by this gene is a cytokine receptor that belongs to the interleukin-1 receptor family. It is mapped to 2q11.2. IL1R2 binds interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I (IL1R1/IL1RA), and acts as a decoy receptor that inhibits the activity of its ligands. IL1R2 also modulates cellular response through non-signaling association with IL1RAP after binding to IL1B. The secreted IL1R2 recruits secreted IL1RAP with high affinity, and this complex formation may be the dominant mechanism for neutralization of IL1B by secreted/soluble receptors.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0396-CAP | Anti-Human IL1R2 Pre-coated 96-well strip microplate | 1 |
| EK0396-ST | Human IL1R2 Standard | 2 vials, 10 ng/tube |
| EK0396-DA | Human IL1R2 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1,000 | 2,000 |
| O.D. | 0.035 | 0.066 | 0.088 | 0.144 | 0.254 | 0.544 | 1.007 | 1.821 |
Data Example Images
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Human IL1R2 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect IL-1 RII, Dilution ratio of 1:20, concentration in serum is 4662-25314 pg/ml..
Some articles we found to cite concentrations of IL-1 RII in samples: 16213746 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human IL1R2/Il 1 Rii ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 100 | 261 | 838 | 99 | 279 | 861 |
| Standard deviation | 7.9 | 14.61 | 42.73 | 7.92 | 17.85 | 57.68 |
| CV (%) | 7.9% | 5.6% | 6.7%% | 8% | 6.4% | 6.7% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of IL-1 RII in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 100 | 94 | 89 | 86 | 92 | 5.3 | 5.7% |
| Sample 2 | 261 | 291 | 293 | 274 | 279 | 13.1 | 4.6% |
| Sample 3 | 838 | 804 | 847 | 848 | 834 | 17.89 | 2.1% |
Protein Target Info & Infographic
Gene/Protein Information For IL1R2 (Source: Uniprot.Org, NCBI)
Gene Name
IL1R2
Full Name
Interleukin-1 receptor type 2
Weight
45421 MW
Superfamily
interleukin-1 receptor family
Alternative Names
Interleukin-1 receptor type 2;IL-1R-2;IL-1RT-2;IL-1RT2;CD121 antigen-like family member B;CDw121b;IL-1 type II receptor;Interleukin-1 receptor beta;IL-1R-beta;Interleukin-1 receptor type II;CD121b;Interleukin-1 receptor type 2, membrane form;mIL-1R2;mIL-1RII;Interleukin-1 receptor type 2, soluble form;sIL-1R2;sIL-1RII;IL1R2;IL1RB; IL1R2 CD121b, CDw121b, IL-1R-2, IL-1RT-2, IL-1RT2c, IL1RB, IL1R2 interleukin 1 receptor type 2 interleukin-1 receptor type 2|CD121 -like family member B|IL-1 type II receptor|IL-1R-beta| CDw121b|interleukin 1 receptor type II variant 3|interleukin-1 receptor beta|interleukin-1 receptor type II|type II interleukin-1 receptor, beta
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on IL1R2, check out the IL1R2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for IL1R2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human IL1R2/Il 1 Rii ELISA Kit PicoKine® (EK0396)
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Human IL1R2/Il 1 Rii ELISA Kit PicoKine®
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis IL-1 RII using the EK0396 Human IL-1 RII Picokine® ELISA Kit?
W. Rodriguez
Verified customer
Asked: 2020-10-16
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to prevent protein degradation.
Boster Scientific Support
Answered: 2020-10-16
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2019-10-03
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2019-10-03
Question
Q: how to thaw whole blood sample for IL-1 RII ELISA after freezing?
B. Scott
Verified customer
Asked: 2019-10-01
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test IL-1 RII for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2019-10-01
Question
Q: Can IL-1 RII ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2017-09-29
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2017-09-29
Question
Q: if the enzyme conjugated IL-1 RII antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the IL-1 RII antigen?
Verified Customer
Verified customer
Asked: 2016-12-01
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2016-12-01
