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Product Info Summary
| SKU: | A04141 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-CD85d LILRB2 Antibody
View all ILT4/CD85d/LILRB2 Antibodies
SKU/Catalog Number
A04141
Size
100μl
Form
Liquid
Description
Boster Bio Anti-CD85d LILRB2 Antibody catalog # A04141. Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD85d LILRB2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04141)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from the Internal region of human LILRB2. AA range:121-170
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04141 is reactive to LILRB2 in Human
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
65039 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04141 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of HeLa cells using CD85d Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For LILRB2 (Source: Uniprot.org, NCBI)
Gene Name
LILRB2
Full Name
Leukocyte immunoglobulin-like receptor subfamily B member 2
Weight
65039 MW
Alternative Names
Leukocyte immunoglobulin-like receptor subfamily B member 2;LIR-2;Leukocyte immunoglobulin-like receptor 2;CD85 antigen-like family member D;Immunoglobulin-like transcript 4;ILT-4;Monocyte/macrophage immunoglobulin-like receptor 10;MIR-10;CD85d;LILRB2;ILT4, LIR2, MIR10; LILRB2 CD85D, ILT-4, ILT4, LIR-2, LIR2, MIR-10, MIR10 leukocyte immunoglobulin like receptor B2 leukocyte immunoglobulin-like receptor subfamily B member 2|CD85 -like family member D|Ig-like transcript 4|leucocyte Ig-like receptor B2|leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 2|monocyte/macrophage immunoglobulin-like receptor 10|myeloid inhibitory receptor 10
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LILRB2, check out the LILRB2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LILRB2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD85d LILRB2 Antibody (A04141)
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Customer Q&As
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13 Customer Q&As for Anti-CD85d LILRB2 Antibody
Question
I see that the anti-CD85d antibody A04141 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-04-17
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-04-17
Question
I have a question about product A04141, anti-CD85d antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-01-15
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04141 anti-CD85d antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-01-15
Question
I have attached the WB image, lot number and protocol we used for dendritic cell peripheral blood monocyte using anti-CD85d antibody A04141. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-12-13
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-13
Question
Is this A04141 anti-CD85d antibody reactive to the isotypes of LILRB2?
Verified Customer
Verified customer
Asked: 2019-11-15
Answer
The immunogen of A04141 anti-CD85d antibody is Synthesized peptide derived from the Internal region of human CD85d. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-11-15
Question
Does A04141 anti-CD85d antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-11-14
Answer
As indicated on the product datasheet, A04141 anti-CD85d antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-11-14
Question
Would anti-CD85d antibody A04141 work for WB with dendritic cell peripheral blood monocyte?
Verified Customer
Verified customer
Asked: 2019-07-10
Answer
According to the expression profile of dendritic cell peripheral blood monocyte, LILRB2 is highly expressed in dendritic cell peripheral blood monocyte. So, it is likely that anti-CD85d antibody A04141 will work for WB with dendritic cell peripheral blood monocyte.
Boster Scientific Support
Answered: 2019-07-10
Question
Do you have a BSA free version of anti-CD85d antibody A04141 available?
Verified Customer
Verified customer
Asked: 2019-05-29
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD85d antibody A04141 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-05-29
Question
Is a blocking peptide available for product anti-CD85d antibody (A04141)?
Verified Customer
Verified customer
Asked: 2019-02-14
Answer
We do provide the blocking peptide for product anti-CD85d antibody (A04141). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-02-14
Question
Does anti-CD85d antibody A04141 work on horse WB with blood?
G. Parker
Verified customer
Asked: 2018-08-09
Answer
Our lab technicians have not tested anti-CD85d antibody A04141 on horse. You can run a BLAST between horse and the immunogen sequence of anti-CD85d antibody A04141 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse blood in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-08-09
Question
We are currently using anti-CD85d antibody A04141 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2018-02-12
Answer
The anti-CD85d antibody (A04141) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-02-12
Question
Our lab want to know about to test anti-CD85d antibody A04141 on human dendritic cell peripheral blood monocyte for research purposes, then I may be interested in using anti-CD85d antibody A04141 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-08-18
Answer
The products we sell, including anti-CD85d antibody A04141, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-08-18
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for dendritic cell peripheral blood monocyte using anti-CD85d antibody A04141. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-07-26
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-07-26
Question
I was wanting to use your anti-CD85d antibody for WB for human dendritic cell peripheral blood monocyte on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human dendritic cell peripheral blood monocyte identification?
K. Rodriguez
Verified customer
Asked: 2017-04-04
Answer
You can see on the product datasheet, A04141 anti-CD85d antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human dendritic cell peripheral blood monocyte in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-04-04
