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Product Info Summary
| SKU: | EK2123 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA, citrate). |
Product info
Product Name
Human LILRB1 ELISA Kit PicoKine®
SKU/Catalog Number
EK2123
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human LILRB1 ELISA Kit PicoKine® (96 Tests). Quantitate Human LILRB1 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 30pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human LILRB1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2123)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: G24-V461
Sensitivity
<30 pg/ml
Assay Range
62.5 pg/ml - 4,000 pg/ml
Standard Dilution Instructions
See datasheet of EK2123 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2123 is reactive to LILRB1 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
Application Guarantee
EK2123 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of LILRB1
Leukocyte immunoglobulin-like receptors (LILRs), also called CD85s, ILTs, or LIRs, are important mediators of immune activation and tolerance that contain tandem immunoglobulin (Ig)-like folds. LILRB1 is encoded within the leukocyte receptor complex on 19q13.4, previously implicated to be a susceptibility region to systemic lupus erythematosus (SLE).LILRB1 binds to a wide range of major histocompatibility complex class I molecules (MHCIs) and transduces negative signals that can, for example, prevent killing of MHCI-expressing cells.LILRB1 transcription is correlated with surface protein expression on NK cells.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2123-CAP | Anti-Human LILRB1 Pre-coated 96-well strip microplate | 1 |
| EK2123-ST | Human LILRB1 Standard | 2 vials, 10 ng/tube |
| EK2123-DA | Human LILRB1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1,000 | 2,000 | 4,000 |
| O.D. | 0.062 | 0.144 | 0.218 | 0.378 | 0.612 | 0.939 | 1.446 | 2.059 |
Data Example Images
Click image to see more details
Human LILRB1 PicoKine ELISA Kit Standard Curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect LILRB1, Dilution ratio of 1:20, concentration in serum and plasma is 5.4-13.6 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human LILRB1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 110 | 162 | 1569 | 116 | 182 | 1572 |
| Standard deviation | 8.34 | 9.95 | 44.56 | 8.97 | 12.94 | 76.08 |
| CV (%) | 7.6% | 6.1% | 2.8% | 7.7% | 7.1% | 4.8% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of LILRB1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 110 | 66 | 64 | 100 | 85 | 3.48 | 4.1% |
| Sample 2 | 162 | 250 | 141 | 229 | 196 | 8.31 | 4.3% |
| Sample 3 | 1569 | 1200 | 1604 | 1340 | 1428 | 54.13 | 3.8% |
Protein Target Info & Infographic
Gene/Protein Information For LILRB1 (Source: Uniprot.Org, NCBI)
Gene Name
LILRB1
Full Name
Leukocyte immunoglobulin-like receptor subfamily B member 1
Weight
70.819kDa
Alternative Names
Leukocyte immunoglobulin-like receptor subfamily B member 1; LIR-1; Leukocyte immunoglobulin-like receptor 1; CD85 antigen-like family member J; Immunoglobulin-like transcript 2; ILT-2; Monocyte/macrophage immunoglobulin-like receptor 7; MIR-7; CD85j; LILRB1; ILT2; LIR1; MIR7 LILRB1 CD85J, ILT-2, ILT2, LIR-1, LIR1, MIR-7, MIR7, PIR-B, PIRB leukocyte immunoglobulin like receptor B1 leukocyte immunoglobulin-like receptor subfamily B member 1|CD85 -like family member J|Ig-like transcript 2|leucocyte Ig-like receptor B1|leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 1|monocyte/macrophage immunoglobulin-like receptor 7|myeloid inhibitory receptor 7
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on LILRB1, check out the LILRB1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for LILRB1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human LILRB1 ELISA Kit PicoKine® (EK2123)
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Customer Q&As
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7 Customer Q&As for Human LILRB1 ELISA Kit PicoKine®
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring LILRB1 using the EK2123 Human LILRB1 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2020-09-13
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to avoid protein degradation.
Boster Scientific Support
Answered: 2020-09-13
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
D. Gandhi
Verified customer
Asked: 2020-06-30
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2020-06-30
Question
Q: how do I analyze ELISA data? I measured LILRB1 level in plasma.
T. Young
Verified customer
Asked: 2019-08-30
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2019-08-30
Question
Q: how can I thaw whole blood sample for LILRB1 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2019-07-20
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test LILRB1 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2019-07-20
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2018-02-16
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2018-02-16
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
S. Walker
Verified customer
Asked: 2017-08-06
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer may impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2017-08-06
Question
Q: if the enzyme conjugated LILRB1 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the LILRB1 antigen?
Verified Customer
Verified customer
Asked: 2014-10-09
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2014-10-09
