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Product Info Summary
| SKU: | EK0389 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma ( heparin, EDTA, citrate). |
Product info
Product Name
Human IL-1 Alpha/IL-1F1/IL1A ELISA Kit PicoKine®
View all IL-1 alpha/IL-1F1 ELISA kits
SKU/Catalog Number
EK0389
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human IL-1 Alpha ELISA Kit PicoKine® (96 Tests). Quantitate Human IL1A in cell culture supernatants, serum and plasma ( heparin, EDTA, citrate). Sensitivity: 0.5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human IL-1 Alpha/IL-1F1/IL1A ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0389)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: S113-A271
Sensitivity
<0.5 pg/ml
Assay Range
4.7 pg/ml - 300 pg/ml
Standard Dilution Instructions
See datasheet of EK0389 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0389 is reactive to IL1A in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma ( heparin, EDTA, citrate).
Application Guarantee
EK0389 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of IL-1 alpha/IL-1F1
Interleukin-1 alpha (IL-1 alpha) and interleukin-1 beta (IL-1 beta) are two biochemically distinct, but distantly related, polypeptidic cytokines that play a key role in inflammation, immunologic reactions, and tissue repair. IL-1 alpha has been implicated in the pathogenesis of infectious, autoimmune and inflammatory diseases. Recently, it has been shown that IL-1 alpha is identical to hematopoietin 1, which is described as a hematopoietic growth factor acting on early progenitor cells in synergy with other hematopoietic growth factors. The human interleukin 1 alpha gene is assigned to chromosome 2. Genetic polymorphisms at interleukin (IL)-1alpha and IL-1beta have been recently suggested to be associated with severity of adult periodontitis. The murine IL-1 alpha and IL-1 beta genes encode structurally and evolutionarily related cytokines that exert a regulatory role in numerous physiological processes including hemopoiesis. The standard product used in this kit is recombinant human IL-1alpha with the molecular mass of 18kDa.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0389-CAP | Anti-Human IL1A Pre-coated 96-well strip microplate | 1 |
| EK0389-ST | Human IL1A Standard | 2 vials, 10 ng/tube |
| EK0389-DA | Human IL1A Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 4.7 | 9.4 | 18.8 | 37.5 | 75 | 150 | 300 |
| O.D. | 0.041 | 0.097 | 0.162 | 0.367 | 0.582 | 0.866 | 1.335 | 2.033 |
Data Example Images
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Human IL-1 alpha PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect IL-1 alpha/IL-1F1, Dilution ratio of 1:1, concentration in serum and plasma is less than the lowest standard, 31.2 pg/ml..
Some articles we found to cite concentrations of IL-1 alpha/IL-1F1 in samples: 25709655 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human IL-1 Alpha/IL-1F1/IL1A ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 14 | 52 | 99 | 15 | 49 | 103 |
| Standard deviation | 0.98 | 2.49 | 4.35 | 1.06 | 2.4 | 6.07 |
| CV (%) | 7% | 4.8% | 4.4% | 7.1% | 4.9% | 5.9% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of IL-1 alpha/IL-1F1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 14 | 15 | 15 | 13 | 14 | 0.82 | 5.8% |
| Sample 2 | 52 | 48 | 50 | 48 | 49 | 1.65 | 3.3% |
| Sample 3 | 99 | 98 | 112 | 102 | 102 | 5.53 | 5.4% |
Protein Target Info & Infographic
Gene/Protein Information For IL1A (Source: Uniprot.Org, NCBI)
Gene Name
IL1A
Full Name
Interleukin-1 alpha
Weight
30607 MW
Superfamily
IL-1 family
Alternative Names
Interleukin-1 alpha;IL-1 alpha;Hematopoietin-1;IL1A;IL1F1; IL1A IL-1 alpha, IL-1A, IL1, IL1-ALPHA, IL1F1 interleukin 1 alpha interleukin-1 alpha|hematopoietin-1|preinterleukin 1 alpha|pro-interleukin-1-alpha
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on IL1A, check out the IL1A Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for IL1A: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human IL-1 Alpha/IL-1F1/IL1A ELISA Kit PicoKine® (EK0389)
Hello CJ!
EK0389 has been cited in 22 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Morphological and functional changes in bone marrow mesenchymal stem cells in rats with heart failure
Interleukins and miRNAs intervene in the early stages of diabetic kidney disease in Type 2 diabetes mellitus patients
Levels of circulating soluble receptor activator of NF-κB and interleukins-1 predicting outcome of locally advanced basal cell carcinoma:
Evaluation of moisturizing and irritation potential of sacha inchi oil
miR‐499 released during myocardial infarction causes endothelial injury by targeting α7‐nAchR
Cathepsin B regulates non‐canonical NLRP3 inflammasome pathway by modulating activation of caspase‐11 in Kupffer cells
LncRNA MALAT1/miR-181a-5p affects the proliferation and adhesion of myeloma cells via regulation of Hippo-YAP signaling pathway
Altered gut microbiota after traumatic splenectomy is associated with endotoxemia
Pro-inflammatory cytokines are associated with podocyte damage and proximal tubular dysfunction in the early stage of diabetic kidney disease in type 2 diabetes mellitus patients
Peripheral proinflammatory cytokines in Chinese patients with generalised anxiety disorder
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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12 Customer Q&As for Human IL-1 Alpha/IL-1F1/IL1A ELISA Kit PicoKine®
Question
Q: is it okay to use citrate plasma as samples in Human IL-1 alpha Picokine® ELISA Kit (Catalog # EK0389)?
Verified Customer
Verified customer
Asked: 2020-10-07
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of IL-1 alpha causing disruption of its protein structure. IL-1 alpha may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the IL-1 alpha ELISA, treating samples with a number of anticoagulants and decided that heparin, EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-10-07
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
N. Huang
Verified customer
Asked: 2020-10-03
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2020-10-03
Question
Q: Can IL-1 alpha ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
R. Turner
Verified customer
Asked: 2020-06-04
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2020-06-04
Question
Dear Technical Support: I purchased Human IL-1 Alpha ELISA Kit PicoKine and would like to ask what the nm value to which the absorbance takes place is?
Verified Customer
Verified customer
Asked: 2020-04-29
Answer
The Human IL-1 Alpha ELISA Kit PicoKine states that absorbance of each well on a spectrophotometer using 450 nm as the primary wavelength and optionally 620 nm (610 nm to 650 nm is acceptable) as the reference wavelength.
Boster Scientific Support
Answered: 2020-04-29
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2019-12-23
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2019-12-23
Question
Q: how to thaw whole blood sample for IL-1 alpha ELISA after freezing?
A. Gandhi
Verified customer
Asked: 2019-03-29
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test IL-1 alpha for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2019-03-29
Question
Q: we need your suggestion regarding the dilution ratio of serum samples for detection of IL-1 alpha in Human plasma? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.
S. Jha
Verified customer
Asked: 2018-11-01
Answer
A: unable to know the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the IL-1 alpha ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2018-11-01
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-10-11
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2018-10-11
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement IL-1 alpha using the EK0389 Human IL-1 alpha Picokine® ELISA Kit?
J. Mehta
Verified customer
Asked: 2018-04-17
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to prevent protein degradation.
Boster Scientific Support
Answered: 2018-04-17
Question
Q: if the enzyme conjugated IL-1 alpha antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the IL-1 alpha antigen?
Verified Customer
Verified customer
Asked: 2016-12-07
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2016-12-07
Question
Q: how do I analyze ELISA data? I have performed IL-1 alpha level in plasma.
H. Jones
Verified customer
Asked: 2016-03-05
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2016-03-05
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
P. Kapoor
Verified customer
Asked: 2015-09-20
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2015-09-20
