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Product Info Summary
| SKU: | EK2074 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Rat |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA, citrate). |
Product info
Product Name
Rat GFRA2 ELISA Kit PicoKine®
View all GFR alpha-2/GDNF R alpha-2 ELISA kits
SKU/Catalog Number
EK2074
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Rat GFRA2 ELISA Kit PicoKine® (96 Tests). Quantitate Rat Gfra2 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Rat GFRA2 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2074)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: S22-S443
Sensitivity
<50 pg/ml
Assay Range
312 pg/ml - 20,000 pg/ml
Standard Dilution Instructions
See datasheet of EK2074 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2074 is reactive to Gfra2 in Rat samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
Application Guarantee
EK2074 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of GFR alpha-2/GDNF R alpha-2
GDNF family receptor alpha-2 (GFRα2), also known as the neurturin receptor, is a protein that in humans is encoded by the GFRA2 gene. It is mapped to 15p11. The GFRA2 protein is a glycosylphosphatidylinositol (GPI)-linked cell surface receptor. It is part of the GDNF receptor family. Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. They both bind the GFRA2 receptor. The receptor mediates activation of the RET tyrosine kinase receptor. This encoded protein acts preferentially as a receptor for NTN compared to its other family member, GDNF family receptor alpha 1. This gene is a candidate gene for RET-associated diseases. GFRA2 (gene) has been shown to interact with GDNF.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2074-CAP | Anti-Rat Gfra2 Pre-coated 96-well strip microplate | 1 |
| EK2074-ST | Rat Gfra2 Standard | 2 vials, 20 ng/tube |
| EK2074-DA | Rat Gfra2 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 312 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 |
| O.D. | 0.072 | 0.117 | 0.199 | 0.249 | 0.678 | 1.090 | 1.434 | 2.227 |
Data Example Images
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Rat GFRA2 PicoKine ELISA Kit Standard Curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Rat GFRA2 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 478 | 1856 | 5549 | 564 | 2008 | 6234 |
| Standard deviation | 22.94 | 96.51 | 299.65 | 34.40 | 126.50 | 411.44 |
| CV (%) | 4.8% | 5.2% | 5.4% | 6.1% | 6.3% | 6.6% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of GFR alpha-2/GDNF R alpha-2 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 478 | 504 | 491 | 512 | 496 | 12.93 | 2.6% |
| Sample 2 | 1856 | 1844 | 1896 | 2084 | 1920 | 96.62 | 5.0% |
| Sample 3 | 5549 | 5354 | 6042 | 6100 | 5761 | 317.99 | 5.5% |
Protein Target Info & Infographic
Gene/Protein Information For Gfra2 (Source: Uniprot.Org, NCBI)
Gene Name
Gfra2
Full Name
GDNF family receptor alpha-2
Weight
51.544kDa
Superfamily
GDNFR family
Alternative Names
GDNF family receptor alpha; Gfra2; GDNFR-beta; rCG_37079 GFRA2 GDNFRB, NRTNR-ALPHA, NTNRA, RETL2, TRNR2 GDNF family receptor alpha 2 GDNF family receptor alpha-2|GDNF receptor beta|GDNFR-alpha-2|GDNFR-beta|GFR-alpha 2|NTNR-alpha|PI-linked cell-surface accessory protein|RET ligand 2|TGF-beta-related neurotrophic factor receptor 2|TRN receptor, GPI-anchored|glial cell line derived neurotrophic factor receptor, beta|neurturin receptor alpha
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Gfra2, check out the Gfra2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Gfra2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Rat GFRA2 ELISA Kit PicoKine® (EK2074)
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Customer Reviews
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Customer Q&As
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8 Customer Q&As for Rat GFRA2 ELISA Kit PicoKine®
Question
Q: Can GFR alpha-2 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
T. Liu
Verified customer
Asked: 2020-12-19
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2020-12-19
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis GFR alpha-2 using the EK2074 Rat GFR alpha-2 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2020-12-08
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to limit protein degradation.
Boster Scientific Support
Answered: 2020-12-08
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2020-04-04
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2020-04-04
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
S. Lee
Verified customer
Asked: 2019-12-11
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-12-11
Question
Q: if the enzyme conjugated GFR alpha-2 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the GFR alpha-2 antigen?
W. Carter
Verified customer
Asked: 2019-04-04
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-04-04
Question
Q: how can I thaw whole blood sample for GFR alpha-2 ELISA after freezing?
Z. Green
Verified customer
Asked: 2018-10-21
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test GFR alpha-2 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2018-10-21
Question
Q: how to analyze ELISA data? I measured GFR alpha-2 level in serum.
V. Yang
Verified customer
Asked: 2018-07-21
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2018-07-21
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2018-04-02
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2018-04-02
