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Product Info Summary
| SKU: | EK0999 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma ( heparin, EDTA). |
Product info
Product Name
Human ICAM-2 ELISA Kit PicoKine®
View all ICAM-2/CD102 ELISA kits
SKU/Catalog Number
EK0999
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human ICAM-2 ELISA Kit PicoKine® (96 Tests). Quantitate Human ICAM2 in cell culture supernatants, serum and plasma ( heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human ICAM-2 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0999)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: K25-Q223
Sensitivity
<10 pg/ml
Assay Range
312 pg/ml - 20,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0999 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0999 is reactive to ICAM2 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma ( heparin, EDTA).
Application Guarantee
EK0999 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of ICAM-2/CD102
Intercellular adhesion molecule 2 (ICAM2), also known as CD102 (Cluster of Differentiation 102) is an integral membrane protein that has 2 immunoglobulin-like domains. This gene is mapped to 17q23.3. The protein encoded by this gene is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0999-CAP | Anti-Human ICAM2 Pre-coated 96-well strip microplate | 1 |
| EK0999-ST | Human ICAM2 Standard | 2 vials, 20 ng/tube |
| EK0999-DA | Human ICAM2 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 312 | 625 | 200000000 | 20000000 | 2000000 | 200000 | 20000 |
| O.D. | 0.118 | 0.232 | 0.343 | 0.508 | 0.910 | 1.463 | 1.951 | 2.583 |
Data Example Images
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Human ICAM-2 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect ICAM-2/CD102, Dilution ratio of 1:10, concentration in serum and plasma is 10.4-41.2 ng/ml..
Some articles we found to cite concentrations of ICAM-2/CD102 in samples: 16245079 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human ICAM-2 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 640 | 2403 | 7918 | 592 | 2447 | 7410 |
| Standard deviation | 38.4 | 151.38 | 443.4 | 38.48 | 196.5% | 437.19 |
| CV (%) | 6% | 8.1%% | 5.6% | 6.5% | 8.1% | 5.9% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of ICAM-2/CD102 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 640 | 642 | 715 | 684 | 670 | 31.24 | 4.6% |
| Sample 2 | 2403 | 2237 | 2185 | 2586 | 2352 | 156.89 | 6.6% |
| Sample 3 | 7918 | 7803 | 8915 | 7900 | 8134 | 453.02 | 5.5% |
Protein Target Info & Infographic
Gene/Protein Information For ICAM2 (Source: Uniprot.Org, NCBI)
Gene Name
ICAM2
Full Name
Intercellular adhesion molecule 2
Weight
30654 MW
Superfamily
immunoglobulin superfamily
Alternative Names
Intercellular adhesion molecule 2;ICAM-2;CD102;ICAM2; ICAM2 CD102 intercellular adhesion molecule 2 intercellular adhesion molecule 2|ICAM-2
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on ICAM2, check out the ICAM2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for ICAM2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human ICAM-2 ELISA Kit PicoKine® (EK0999)
Hello CJ!
EK0999 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Xu Y, Feng L, Wang S, Zhu Q, Lin J, Lou C, Xiang P, He B, Zheng Z, Tang D, Zuo G. J Cell Biochem. 2011 Oct;112(10):2953-65. Doi: 10.1002/Jcb.23212. Phytoestrogen Calycosin-7-O-??-D-Glucopyranoside Ameliorates Advanced Glycation End Products-Induce...
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Customer Q&As
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7 Customer Q&As for Human ICAM-2 ELISA Kit PicoKine®
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2020-11-19
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2020-11-19
Question
Q: is it okay to use citrate plasma as samples in Human CD102 Picokine® ELISA Kit (Catalog # EK0999)?
Verified Customer
Verified customer
Asked: 2019-08-25
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of CD102 causing disruption of its protein structure. CD102 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the CD102 ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2019-08-25
Question
Q: if the enzyme conjugated CD102 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CD102 antigen?
Verified Customer
Verified customer
Asked: 2019-05-16
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-05-16
Question
Q: how to analyze ELISA data? I measured CD102 level in plasma.
Verified Customer
Verified customer
Asked: 2019-03-27
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2019-03-27
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement CD102 using the EK0999 Human CD102 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-09-04
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples in order to limit protein degradation.
Boster Scientific Support
Answered: 2018-09-04
Question
Q: which procedure should I follow in order to thaw whole blood sample for CD102 ELISA after freezing?
T. Zhou
Verified customer
Asked: 2018-05-06
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test CD102 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2018-05-06
Question
Q: we need your recommendation regarding the dilution ratio of serum samples for detection of CD102 in Human plasma? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2017-05-29
Answer
A: unable to know the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the CD102 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2017-05-29
