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Product Info Summary
| SKU: | EK0753 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Mouse ESM1/Endocan ELISA Kit PicoKine®
View all Endocan/ESM-1 ELISA kits
SKU/Catalog Number
EK0753
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse ESM1/Endocan ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Esm1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse ESM1/Endocan ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0753)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: W20-R184
Sensitivity
<10 pg/ml
Assay Range
23.4 pg/ml - 1500 pg/ml
Standard Dilution Instructions
See datasheet of EK0753 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0753 is reactive to Esm1 in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0753 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Endocan/ESM-1
Endothelial cell-specific molecule 1, also known as Endocan, is a protein that in humans is encoded by the ESM1 gene. This gene encodes a secreted protein which is mainly expressed in the endothelial cells in human lung and kidney tissues. The expression of this gene is regulated by cytokines, suggesting that it may play a role in endothelium-dependent pathological disorders. The transcript contains multiple polyadenylation and mRNA instability signals. ESM-1 has been described as a specific biomarker of tip cells during neoangiogenesis by independent teams. Its expression has been shown to be increase in presence of pro-angiogenic growth factors such as VEGF (vascular endothelial growth factor) or FGF-2 (fibroblast growth factor 2).
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0753-CAP | Anti-Mouse Esm1 Pre-coated 96-well strip microplate | 1 |
| EK0753-ST | Mouse Esm1 Standard | 2 vials, 10 ng/tube |
| EK0753-DA | Mouse Esm1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 23.4 | 46.8 | 93.7 | 187.5 | 375 | 750 | 1500 |
| O.D. | 0.032 | 0.137 | 0.228 | 0.414 | 0.740 | 1.302 | 1.649 | 2.047 |
Data Example Images
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Mouse ESM1/Endocan PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Endocan/ESM-1, Dilution ratio of 1:5, concentration in serum and plasma is 400-2000 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse ESM1/Endocan ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 56 | 169 | 683 | 60 | 163 | 636 |
| Standard deviation | 3.36 | 10.14 | 52.59 | 4.2 | 12.22 | 56.6 |
| CV (%) | 6% | 6% | 7.7% | 7% | 7.5% | 8.9% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Endocan/ESM-1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 56 | 53 | 49 | 52 | 52 | 2.5 | 4.8% |
| Sample 2 | 169 | 162 | 178 | 184 | 173 | 8.4 | 4.8% |
| Sample 3 | 683 | 675 | 706 | 643 | 676 | 22.56 | 3.3% |
Protein Target Info & Infographic
Gene/Protein Information For Esm1 (Source: Uniprot.Org, NCBI)
Gene Name
Esm1
Full Name
Endothelial cell-specific molecule 1
Weight
20043 MW
Alternative Names
Endothelial cell-specific molecule 1;ESM-1;Esm1; ESM1 endocan endothelial cell specific molecule 1 endothelial cell-specific molecule 1|ESM-1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Esm1, check out the Esm1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Esm1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse ESM1/Endocan ELISA Kit PicoKine® (EK0753)
Hello CJ!
EK0753 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
ESM1 mediates NGFR-induced invasion and metastasis in murine oral squamous cell carcinoma
ESM1 mediates NGFR-induced invasion and metastasis in murine oral squamous cell carcinoma
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Customer Q&As
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8 Customer Q&As for Mouse ESM1/Endocan ELISA Kit PicoKine®
Question
Q: if the enzyme conjugated Endocan antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Endocan antigen?
Z. Jackson
Verified customer
Asked: 2020-08-17
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2020-08-17
Question
Q: how to proceed with the analysis of ELISA data? I measured Endocan level in plasma.
K. Jha
Verified customer
Asked: 2020-03-16
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2020-03-16
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement Endocan using the EK0753 Mouse Endocan Picokine® ELISA Kit?
T. Walker
Verified customer
Asked: 2019-11-04
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to limit protein degradation.
Boster Scientific Support
Answered: 2019-11-04
Question
Q: can I use citrate plasma as samples in Mouse Endocan Picokine® ELISA Kit (Catalog # EK0753)?
T. Lopez
Verified customer
Asked: 2019-10-16
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of Endocan causing disruption of its protein structure. Endocan may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the Endocan ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2019-10-16
Question
Q: which procedure should I follow in order to thaw whole blood sample for Endocan ELISA after freezing?
Verified Customer
Verified customer
Asked: 2019-03-24
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Endocan for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2019-03-24
Question
Q: What is the optimal O.D. value for Endocan ELISA kit? I performed your Endocan ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain Endocan even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2018-10-17
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. considering your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Endocan ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of Endocan that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2018-10-17
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2017-11-13
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2017-11-13
Question
Q: can you suggest the dilution ratio of serum samples for detection of Endocan in Mouse plasma? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.
Z. Lopez
Verified customer
Asked: 2017-07-24
Answer
A: unable to know the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Endocan ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2017-07-24
