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Product Info Summary
| SKU: | EK0770 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | culture supernatants, cell lysates, serum, plasma (heparin, EDTA), saliva, urine and human milk. |
Product info
Product Name
Human HBEGF ELISA Kit PicoKine®
SKU/Catalog Number
EK0770
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human HBEGF ELISA Kit PicoKine® (96 Tests). Quantitate Human HBEGF in cell culture supernatants, cell lysates, serum, plasma (heparin, EDTA), saliva, urine and human milk. Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human HBEGF ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0770)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: D63-L148
Sensitivity
<2 pg/ml
Assay Range
7.8 pg/ml - 500 pg/ml
Standard Dilution Instructions
See datasheet of EK0770 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0770 is reactive to HBEGF in Human samples
Validated Sample Types
culture supernatants, cell lysates, serum, plasma (heparin, EDTA), saliva, urine and human milk.
Application Guarantee
EK0770 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of HB-EGF
HBEGF (Heparin-binding EGF-like growth factor), also known as HEGFL or DTR, is a member of the EGF family of proteins that in humans is encoded by the HBEGF gene. The HBEGF gene is assigned to chromosome 5, thus confirming the assignment of the gene on the basis of its role in relation to diphtheria toxin susceptibility. HB-EGF is an 87 amino acid glycoprotein which displays highly regulated gene expression. It has been shown to play a role in wound healing, cardiac hypertrophy and heart development and function. HB-EGF binding and activation of EGF receptors plays a critical role during cardiac valve tissue development and the maintenance of normal heart function in adults.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0770-CAP | Anti-Human HBEGF Pre-coated 96-well strip microplate | 1 |
| EK0770-ST | Human HBEGF Standard | 2 vials, 10 ng/tube |
| EK0770-DA | Human HBEGF Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 7.8 | 15.6 | 31.3 | 62.5 | 125 | 250 | 500 |
| O.D. | 0.029 | 0.063 | 0.099 | 0.181 | 0.323 | 0.643 | 1.307 | 2.025 |
Data Example Images
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Human HBEGF PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect HB-EGF, For serum, dilution ratio is 1:1 and concentration is 36-451 pg/ml.
For plasma, dilution ratio is 1:1 and concentration is 0-114 pg/ml..
Some articles we found to cite concentrations of HB-EGF in samples: 9618289 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human HBEGF ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 16 | 77 | 282 | 15 | 70 | 280 |
| Standard deviation | 1 | 3.77 | 11.56 | 1.05 | 4.2 | 11.76 |
| CV (%) | 6% | 4.9% | 4.1% | 7% | 6% | 4.2% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of HB-EGF in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 16 | 14 | 15 | 15 | 15 | 0.7 | 4.6% |
| Sample 2 | 77 | 73 | 73 | 83 | 76 | 4.09 | 5.3% |
| Sample 3 | 282 | 268 | 286 | 279 | 278 | 6.68 | 2.4% |
Protein Target Info & Infographic
Gene/Protein Information For HBEGF (Source: Uniprot.Org, NCBI)
Gene Name
HBEGF
Full Name
Proheparin-binding EGF-like growth factor
Weight
23067 MW
Alternative Names
Proheparin-binding EGF-like growth factor;Heparin-binding EGF-like growth factor;HB-EGF;HBEGF;Diphtheria toxin receptor;DT-R;HBEGF;DTR, DTS, HEGFL; HBEGF DTR, DTS, DTSF, HEGFL heparin binding EGF like growth factor proheparin-binding EGF-like growth factor|diphtheria toxin receptor (heparin-binding EGF-like growth factor)|diphtheria toxin receptor (heparin-binding epidermal growth factor-like growth factor)|heparin-binding epidermal growth factor
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on HBEGF, check out the HBEGF Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for HBEGF: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human HBEGF ELISA Kit PicoKine® (EK0770)
Hello CJ!
EK0770 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
GABABR-Induced EGFR Transactivation Promotes Migration of Human Prostate Cancer Cells
The Adhesion AND Growth of Both the Human Primary Gingival Epithelial Cells and Streptococcus Mutans on Micro-Arc Oxidized Titanium
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Customer Reviews
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Customer Q&As
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10 Customer Q&As for Human HBEGF ELISA Kit PicoKine®
Question
Q: Can HB-EGF ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2020-10-07
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2020-10-07
Question
Q: how do I analyze ELISA data? I measured HB-EGF level in serum.
Verified Customer
Verified customer
Asked: 2020-09-08
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2020-09-08
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
L. Martin
Verified customer
Asked: 2020-07-17
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2020-07-17
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2020-01-31
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can change immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2020-01-31
Question
Q: how can I thaw whole blood sample for HB-EGF ELISA after freezing?
M. Robinson
Verified customer
Asked: 2019-09-29
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test HB-EGF for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2019-09-29
Question
Q: can I use citrate plasma as samples in Human HB-EGF Picokine® ELISA Kit (Catalog # EK0770)?
Verified Customer
Verified customer
Asked: 2019-06-02
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of HB-EGF causing disruption of its protein structure. HB-EGF may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the HB-EGF ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2019-06-02
Question
Q: What is the optimal O.D. value for HB-EGF ELISA kit? I used your HB-EGF ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain HB-EGF even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2018-12-15
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. in the above example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this HB-EGF ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of HB-EGF that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2018-12-15
Question
Q: can you recommend the dilution ratio of serum samples for detection of HB-EGF in Human serum? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.
Y. Edwards
Verified customer
Asked: 2018-10-21
Answer
A: without knowing the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the HB-EGF ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2018-10-21
Question
Hello, I'm a customer of EK0770 and EK1378. I want to detect HB-EGF, NRG-1 levels of SK-BR-3, HER2 positive breast cancer cell line. In the protocol, there is any information about the proper number or density of cells for ELISA assay (for Cell culure supernants and Cell lysates). Would you advise me on the number of cells I should prepare? Thank you for your help.
Verified Customer
Verified customer
Asked: 2017-05-08
Answer
The number of cells we recommened for cell culure supernants and cell lysates is 106 cells/mL.
Boster Scientific Support
Answered: 2017-05-08
Question
Q: if the enzyme conjugated HB-EGF antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the HB-EGF antigen?
Verified Customer
Verified customer
Asked: 2016-09-11
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2016-09-11
