HB-EGF Proheparin-Binding EGF-like Growth Factor Mouse Recombinant Protein

Product Name

HB-EGF Proheparin-Binding EGF-like Growth Factor Mouse Recombinant Protein

View all HB-EGF recombinant proteins

SKU/Catalog Number

PROTQ06186

Size

10ug, 50ug, 1mg

Description

HB-EGF Mouse Recombinant produced in E. coli is a single, non-glycosylated, polypeptide chain containing 86 amino acids (63-148 a.a.) and having a molecular mass of 9.8 kDa. The HB-EGF is purified by proprietary chromatographic techniques.

Storage & Handling

Lyophilized Mouse HB-EGF Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution HB-EGF should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Cite This Product

HB-EGF Proheparin-Binding EGF-like Growth Factor Mouse Recombinant Protein (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTQ06186)

Form

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

The protein was filtered (0.2µm) and lyophilized from a concentrated solution containing 10mM PB and 500mM NaCl, pH7.4.

Purity

Greater than 97.0% as determined by (a) Analysis by RP-HPLC and (b) Analysis by SDS-PAGE.

Predicted MW

23.067kDa

Reconstitution

It is recommended to reconstitute the lyophilized Mouse HB-EGF in sterile 18M-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.

Biological Activity

The ED50 was determined by a cell proliferation assay using balb/c 3T3 cells is less than 1.0 ng/ml, corresponding to a specific activity of greater than 1.0×10⁶ units/mg.

Reconstitution

It is recommended to reconstitute the lyophilized Mouse HB-EGF in sterile 18M-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.

Validation Images & Assay Conditions

Gene/Protein Information For HBEGF (Source: Uniprot.org, NCBI)

Alternative Names

DTR; HEGFL; diphtheria toxin receptor; DTSF HBEGF DTR, DTS, DTSF, HEGFL heparin binding EGF like growth factor proheparin-binding EGF-like growth factor|diphtheria toxin receptor (heparin-binding EGF-like growth factor)|diphtheria toxin receptor (heparin-binding epidermal growth factor-like growth factor)|heparin-binding epidermal growth factor

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

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