HB-EGF Proheparin-Binding EGF-like Growth Factor Rat Recombinant Protein

Product Name

HB-EGF Proheparin-Binding EGF-like Growth Factor Rat Recombinant Protein

View all HB-EGF recombinant proteins

SKU/Catalog Number

PROTQ06175

Size

10ug, 50ug, 1mg

Description

HB-EGF Rat Recombinant produced in E. coli is a single, non-glycosylated, polypeptide chain containing 86 amino acids and having a molecular mass of 9.7kDa. The HB-EGF is purified by proprietary chromatographic techniques.

Storage & Handling

Lyophilized Rat HB-EGF Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution HB-EGF should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Cite This Product

HB-EGF Proheparin-Binding EGF-like Growth Factor Rat Recombinant Protein (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTQ06175)

Form

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

The protein was filtered (0.2µm) and lyophilized from a concentrated solution containing PBS, 300mM NaCl, pH 7.4 and 5% trehalose.

Purity

Greater than 95.0% as determined by (a) Analysis by RP-HPLC and (b) Analysis by SDS-PAGE.

Predicted MW

23.067kDa

Reconstitution

It is recommended to reconstitute the lyophilized Rat HB-EGF in sterile 18M-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.

Biological Activity

The ED₅₀ as determined by a cell proliferation assay using balb/c 3T3 cells is less than 1.0 ng/ml, corresponding to a specific activity of greater than 1.0×10⁶ units/mg.

Reconstitution

It is recommended to reconstitute the lyophilized Rat HB-EGF in sterile 18M-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.

Validation Images & Assay Conditions

Gene/Protein Information For HBEGF (Source: Uniprot.org, NCBI)

Alternative Names

Proheparin-binding EGF-like growth factor; Heparin-binding EGF-like growth factor; HB-EGF; HBEGF; Dtr; Hegfl; GFHB HBEGF DTR, DTS, DTSF, HEGFL heparin binding EGF like growth factor proheparin-binding EGF-like growth factor|diphtheria toxin receptor (heparin-binding EGF-like growth factor)|diphtheria toxin receptor (heparin-binding epidermal growth factor-like growth factor)|heparin-binding epidermal growth factor

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis