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Product Info Summary
| SKU: | A03415 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Galectin-9 LGALS9 Antibody
View all Galectin-9 Antibodies
SKU/Catalog Number
A03415
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Galectin-9 LGALS9 Antibody catalog # A03415. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Galectin-9 LGALS9 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03415)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human LEG9. AA range:51-100
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A03415 is reactive to LGALS9 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
39518 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03415 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of HepG2 cells using Galectin-9 Polyclonal antibody.
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Western Blot (WB) analysis of specific cells using Galectin-9 Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For LGALS9 (Source: Uniprot.org, NCBI)
Gene Name
LGALS9
Full Name
Galectin-9
Weight
39518 MW
Alternative Names
Galectin-9;Gal-9;Ecalectin;Tumor antigen HOM-HD-21;LGALS9; LGALS9 HUATA, LGALS9 galectin 9 galectin-9|ecalectin|gal-9|lectin, galactoside-binding, soluble, 9|tumor HOM-HD-21|urate transporter/channel protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LGALS9, check out the LGALS9 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LGALS9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Galectin-9 LGALS9 Antibody (A03415)
Hello CJ!
A03415 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Galectin-3 and Galectin-9 May Differently Regulate the Expressions of Microglial M1/M2 Markers and T Helper 1/Th2 Cytokines in the Brains of Genetically Susceptible C57BL/6 and Resistant BALB/c Mice Following Peroral Infection With Toxoplasma gondii
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-Galectin-9 LGALS9 Antibody
Question
We are currently using anti-Galectin-9 antibody A03415 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-07
Answer
The anti-Galectin-9 antibody (A03415) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-07
Question
See below the WB image, lot number and protocol we used for kidney stomach using anti-Galectin-9 antibody A03415. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-04-03
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-03
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for kidney stomach using anti-Galectin-9 antibody A03415. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-02-28
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-28
Question
Is this A03415 anti-Galectin-9 antibody reactive to the isotypes of LGALS9?
Verified Customer
Verified customer
Asked: 2020-02-26
Answer
The immunogen of A03415 anti-Galectin-9 antibody is Synthesized peptide derived from the Internal region of human Galectin-9. at AA rangle: 30-110. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-02-26
Question
My team were satisfied with the WB result of your anti-Galectin-9 antibody. However we have been able to see positive staining in kidney stomach isoform 3: secreted using this antibody. Is that expected? Could you tell me where is LGALS9 supposed to be expressed?
E. Collins
Verified customer
Asked: 2020-02-25
Answer
Based on literature, kidney stomach does express LGALS9. Generally LGALS9 expresses in cytoplasm., isoform 2: secreted, isoform 3: secreted. Regarding which tissues have LGALS9 expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 15489334
Gastric mucosa, Pubmed ID: 16625196
Spleen, Pubmed ID: 9045665
Boster Scientific Support
Answered: 2020-02-25
Question
We have been able to see staining in rat mucosa of transverse colon. Do you have any suggestions? Is anti-Galectin-9 antibody supposed to stain mucosa of transverse colon positively?
Verified Customer
Verified customer
Asked: 2020-01-21
Answer
According to literature mucosa of transverse colon does express LGALS9. According to Uniprot.org, LGALS9 is expressed in mucosa of transverse colon, spleen, gastric carcinoma, kidney stomach, gastric mucosa, colon, among other tissues. Regarding which tissues have LGALS9 expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 15489334
Gastric mucosa, Pubmed ID: 16625196
Spleen, Pubmed ID: 9045665
Boster Scientific Support
Answered: 2020-01-21
Question
My lab would like to test anti-Galectin-9 antibody A03415 on rat kidney stomach for research purposes, then I may be interested in using anti-Galectin-9 antibody A03415 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-12-06
Answer
The products we sell, including anti-Galectin-9 antibody A03415, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-12-06
Question
Can you help my question with product A03415, anti-Galectin-9 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-09-24
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A03415 anti-Galectin-9 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-09-24
Question
I was wanting to use your anti-Galectin-9 antibody for WB for rat kidney stomach on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat kidney stomach identification?
Verified Customer
Verified customer
Asked: 2019-09-06
Answer
As indicated on the product datasheet, A03415 anti-Galectin-9 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat kidney stomach in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-06
Question
Will anti-Galectin-9 antibody A03415 work for WB with kidney stomach?
Verified Customer
Verified customer
Asked: 2019-08-08
Answer
According to the expression profile of kidney stomach, LGALS9 is highly expressed in kidney stomach. So, it is likely that anti-Galectin-9 antibody A03415 will work for WB with kidney stomach.
Boster Scientific Support
Answered: 2019-08-08
Question
Is a blocking peptide available for product anti-Galectin-9 antibody (A03415)?
Verified Customer
Verified customer
Asked: 2019-07-26
Answer
We do provide the blocking peptide for product anti-Galectin-9 antibody (A03415). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-07-26
Question
Is there a BSA free version of anti-Galectin-9 antibody A03415 available?
Verified Customer
Verified customer
Asked: 2018-04-25
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Galectin-9 antibody A03415 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-04-25
Question
I see that the anti-Galectin-9 antibody A03415 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-04-12
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-04-12
Question
Would A03415 anti-Galectin-9 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-06-01
Answer
It shows on the product datasheet, A03415 anti-Galectin-9 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-06-01
Question
I am interested in using your anti-Galectin-9 antibody for positive regulation of cd4-positive studies. Has this antibody been tested with western blotting on hepg2 cells? We would like to see some validation images before ordering.
D. Collins
Verified customer
Asked: 2013-01-04
Answer
We appreciate your inquiry. This A03415 anti-Galectin-9 antibody is validated on hepg2 cells. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2013-01-04
