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Product Info Summary
| SKU: | EK1468 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human Fibulin-3/EFEMP1 ELISA Kit PicoKine®
View all Fibulin-3/EFEMP1 ELISA kits
SKU/Catalog Number
EK1468
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Fibulin-3/EFEMP1 ELISA Kit PicoKine® (96 Tests). Quantitate Human EFEMP1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Fibulin-3/EFEMP1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1468)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: CHO; Immunogen sequence: Q18-F493
Sensitivity
<10 pg/ml
Assay Range
312 pg/ml - 20,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1468 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1468 is reactive to EFEMP1 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK1468 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Fibulin-3/EFEMP1
EGF-containing fibulin-like extracellular matrix protein 1 is a protein that in humans is encoded by the EFEMP1 gene. This gene encodes a member of the fibulin family of extracellular matrix glycoproteins. Like all members of this family, the encoded protein contains tandemly repeated epidermal growth factor-like repeats followed by a C-terminus fibulin-type domain. This gene is upregulated in malignant gliomas and may play a role in the aggressive nature of these tumors. Mutations in this gene are associated with Doyne honeycomb retinal dystrophy. Alternatively spliced transcript variants that encode the same protein have been described.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1468-CAP | Anti-Human EFEMP1 Pre-coated 96-well strip microplate | 1 |
| EK1468-ST | Human EFEMP1 Standard | 2 vials, 20 ng/tube |
| EK1468-DA | Human EFEMP1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 312 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 |
| O.D. | 0.015 | 0.086 | 0.138 | 0.260 | 0.524 | 0.838 | 1.437 | 2.058 |
Data Example Images
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Human Fibulin-3/EFEMP1 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Fibulin-3/EFEMP1, Dilution ratio of 1:1000, concentration in serum is 1500-2800ng/ml.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Fibulin-3/EFEMP1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 650 | 2080 | 10283 | 629 | 2055 | 10529 |
| Standard deviation | 31.2 | 84.88 | 473.01 | 37.11 | 117.13 | 610.68 |
| CV (%) | 4.8% | 4.1% | 4.6% | 5.9% | 5.7% | 5.8% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Fibulin-3/EFEMP1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 650 | 662 | 670 | 638 | 655 | 12.12 | 1.8% |
| Sample 2 | 2080 | 2103 | 2020 | 2397 | 2150 | 145.78 | 6.7% |
| Sample 3 | 10283 | 8683 | 9084 | 9862 | 9478 | 629.03 | 6.6% |
Protein Target Info & Infographic
Gene/Protein Information For EFEMP1 (Source: Uniprot.Org, NCBI)
Gene Name
EFEMP1
Full Name
EGF-containing fibulin-like extracellular matrix protein 1
Weight
54641 MW
Superfamily
fibulin family
Alternative Names
EGF-containing fibulin-like extracellular matrix protein 1;Extracellular protein S1-5;Fibrillin-like protein;Fibulin-3;FIBL-3;EFEMP1;FBLN3, FBNL; EFEMP1 DHRD, DRAD, FBLN3, FBNL, FIBL-3, MLVT, MTLV, S1-5 EGF containing fibulin extracellular matrix protein 1 EGF-containing fibulin-like extracellular matrix protein 1|EGF containing fibulin like extracellular matrix protein 1|extracellular protein S1-5|fibulin-3
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on EFEMP1, check out the EFEMP1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for EFEMP1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Fibulin-3/EFEMP1 ELISA Kit PicoKine® (EK1468)
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Customer Reviews
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Customer Q&As
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10 Customer Q&As for Human Fibulin-3/EFEMP1 ELISA Kit PicoKine®
Question
Q: can I use citrate plasma as samples in Human Fibulin-3 Picokine® ELISA Kit (Catalog # EK1468)?
Verified Customer
Verified customer
Asked: 2020-03-08
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of Fibulin-3 causing degradation of its protein structure. Fibulin-3 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the Fibulin-3 ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-03-08
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
W. Chakrabarti
Verified customer
Asked: 2020-02-10
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2020-02-10
Question
I just wanted to confirm if we had enough EK1468 lot # 10101487824 to reserve 15 kits instead of 10?
Verified Customer
Verified customer
Asked: 2019-12-26
Answer
We have enough EK1468 lot # 10101487824 to reserve 15 kits.
Boster Scientific Support
Answered: 2019-12-26
Question
Q: how do I analyze ELISA data? I have performed Fibulin-3 level in plasma.
G. Zhou
Verified customer
Asked: 2019-10-02
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2019-10-02
Question
Q: if the enzyme conjugated Fibulin-3 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Fibulin-3 antigen?
K. Roberts
Verified customer
Asked: 2019-08-02
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-08-02
Question
What is the standard of EK1468 kit made of and are there any animal side-products in the kit?
Verified customer
Asked: 2019-07-15
Answer
The standard of the Human Fibulin-3/EFEMP1 ELISA Kit PicoKine EK1468 is a recombinant protein and some of the kit components contain BSA.
Boster Scientific Support
Answered: 2019-07-16
Question
Q: we need your recommendation regarding the dilution ratio of serum samples for detection of Fibulin-3 in Human plasma? I am trying to measure a a number of analytes and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.
W. Phillips
Verified customer
Asked: 2019-07-11
Answer
A: unable to know the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Fibulin-3 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2019-07-11
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2019-03-18
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2019-03-18
Question
The customer may want to reserve kits, do we have ten EK1468 lot # 10101487824 in stock?
Verified Customer
Verified customer
Asked: 2016-04-04
Answer
We have ten EK1468 lot # 10101487824 in stock.
Boster Scientific Support
Answered: 2016-04-04
Question
Q: What is the optimal O.D. value for Fibulin-3 ELISA kit? I performed your Fibulin-3 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain Fibulin-3 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2015-10-02
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. considering your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Fibulin-3 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of Fibulin-3 that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2015-10-02
