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Product Info Summary
| SKU: | A02302-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-Fibulin 3 EFEMP1 Antibody
View all Fibulin-3/EFEMP1 Antibodies
SKU/Catalog Number
A02302-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Fibulin 3 EFEMP1 Antibody (Catalog # A02302-1). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Fibulin 3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Fibulin 3 EFEMP1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02302-1)
Host
Rabbit
Contents
Fibulin 3 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Fibulin 3 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human Fibulin 3. The immunogen is located within amino acids 410 - 460 of Fibulin 3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least three isoforms of Fibulin 3 are known to exist. This antibody is predicted to not cross-react with other Fibulin proteins.
Reactive Species
A02302-1 is reactive to EFEMP1 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
54641 MW
Background of Fibulin-3/EFEMP1
Fibulin 3, also known as epidermal growth factor (EGF)-containing fibulin-like extracellular matrix protein 1 (EFEMP1), is a member of the fibulin family of extracellular glycoproteins, a group of proteins that are widely distributed and frequently associated with vascular and elastic tissues. The fibulin proteins typically contain a tandem array of EGF-like repeats and a fibulin-type COOH-terminal module. Aberrant accumulation of Fibulin 3 in the endoplasmic reticulum of retinal pigment epithelial cells has been shown to be associated with inherited forms of macular degeneration, but the loss of Fibulin 3 expression does not lead to macular degeneration but rather the appearance of hernias due to a reduction of elastic fibers of fascial connective tissue. Recent experiments have shown that expression of Fibulin 3 promotes tumor growth and may thus be a therapeutic target.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02302-1 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Fibulin 3 antibody can be used for detection of Fibulin 3 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of Fibulin 3 in HeLa cell lysate with Fibulin 3 antibody at (A) 0.5 and (B) 1 μg/mL.
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Immunocytochemistry of Fibulin 3 in HeLa cells with Fibulin 3 antibody at 20 μg/mL.
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Immunofluorescence of Fibulin 3 in HeLa cells with Fibulin 3 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For EFEMP1 (Source: Uniprot.org, NCBI)
Gene Name
EFEMP1
Full Name
EGF-containing fibulin-like extracellular matrix protein 1
Weight
54641 MW
Superfamily
fibulin family
Alternative Names
DHRD, DRAD, FBNL, MLVT, MTLV, S1-5, FBLN3, FIBL-3, EGF-containing fibulin-like extracellular matrix protein 1, Extracellular protein S1-5 EFEMP1 DHRD, DRAD, FBLN3, FBNL, FIBL-3, MLVT, MTLV, S1-5 EGF containing fibulin extracellular matrix protein 1 EGF-containing fibulin-like extracellular matrix protein 1|EGF containing fibulin like extracellular matrix protein 1|extracellular protein S1-5|fibulin-3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EFEMP1, check out the EFEMP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EFEMP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Fibulin 3 EFEMP1 Antibody (A02302-1)
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