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Product Info Summary
| SKU: | A02302 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Fibulin-3 EFEMP1 Antibody
View all Fibulin-3/EFEMP1 Antibodies
SKU/Catalog Number
A02302
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Fibulin-3 EFEMP1 Antibody catalog # A02302. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Fibulin-3 EFEMP1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02302)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human EFEMP1. AA range:111-160
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02302 is reactive to EFEMP1 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
54641 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02302 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of rat cells using Fibulin-3 Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For EFEMP1 (Source: Uniprot.org, NCBI)
Gene Name
EFEMP1
Full Name
EGF-containing fibulin-like extracellular matrix protein 1
Weight
54641 MW
Superfamily
fibulin family
Alternative Names
EGF-containing fibulin-like extracellular matrix protein 1;Extracellular protein S1-5;Fibrillin-like protein;Fibulin-3;FIBL-3;EFEMP1;FBLN3, FBNL; EFEMP1 DHRD, DRAD, FBLN3, FBNL, FIBL-3, MLVT, MTLV, S1-5 EGF containing fibulin extracellular matrix protein 1 EGF-containing fibulin-like extracellular matrix protein 1|EGF containing fibulin like extracellular matrix protein 1|extracellular protein S1-5|fibulin-3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EFEMP1, check out the EFEMP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EFEMP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Fibulin-3 EFEMP1 Antibody (A02302)
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Customer Q&As
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4 Customer Q&As for Anti-Fibulin-3 EFEMP1 Antibody
Question
We are currently using anti-Fibulin-3 antibody A02302 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on dog tissues as well?
K. Banerjee
Verified customer
Asked: 2020-01-15
Answer
The anti-Fibulin-3 antibody (A02302) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-15
Question
Can you help my question with product A02302, anti-Fibulin-3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-12-02
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A02302 anti-Fibulin-3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-12-02
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-Fibulin-3 antibody A02302. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-05-21
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-21
Question
See below the WB image, lot number and protocol we used for liver using anti-Fibulin-3 antibody A02302. Please let me know if you require anything else.
M. Dhar
Verified customer
Asked: 2017-11-22
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-11-22
