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Product Info Summary
| SKU: | EK0930 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Mouse IL-33 ELISA Kit PicoKine®
SKU/Catalog Number
EK0930
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse IL-33 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Il33 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse IL-33 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0930)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: S109-I266
Sensitivity
<10 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0930 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0930 is reactive to Il33 in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0930 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of IL-33
Interleukin 33 (IL-33) is a cytokine belonging to the IL-1 superfamily. By genomic sequence analysis, the IL33 gene is mapped to chromosome 9p24.1 and its mouse homolog to a syntenic region on chromosome 19qC1. The induction of type 2 cytokines by IL-33 in vivo is believed to induce the severe pathological changes observed in mucosal organs following administration of IL-33. IL33, an alarmin released from necrotic cells, is necessary for potent CD8 + T cell (CTL) responses to replicating, prototypic RNA, and DNA viruses in mice. IL33 prevented the downregulation of CXCR2 and inhibition of chemotaxis induced by activation of TLR4, and found that IL33 reverses the TLR4-induced reduction of CXCR2 expression via the inhibition of expression of GRK2.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0930-CAP | Anti-Mouse Il33 Pre-coated 96-well strip microplate | 1 |
| EK0930-ST | Mouse Il33 Standard | 2 vials, 10 ng/tube |
| EK0930-DA | Mouse Il33 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.016 | 0.070 | 0.122 | 0.234 | 0.450 | 0.909 | 1.536 | 2.094 |
Data Example Images
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Mouse IL-33 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect IL-33, Dilution ratio of 1:1, concentration in serum and plasma is 0-260 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse IL-33 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 63 | 290 | 939 | 67 | 300 | 883 |
| Standard deviation | 4.72 | 22.62 | 44.13 | 7.52 | 27.6 | 50.33 |
| CV (%) | 7.5% | 7.8% | 4.7% | 7.8% | 9.2% | 5.7% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of IL-33 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 63 | 56 | 58 | 57 | 58 | 2.69 | 4.6% |
| Sample 2 | 290 | 253 | 299 | 266 | 277 | 18.37 | 6.6% |
| Sample 3 | 939 | 813 | 894 | 950 | 899 | 53.9 | 5.9% |
Protein Target Info & Infographic
Gene/Protein Information For Il33 (Source: Uniprot.Org, NCBI)
Gene Name
Il33
Full Name
Interleukin-33
Weight
29991 MW
Superfamily
IL-1 family
Alternative Names
Interleukin-33;IL-33;Interleukin-33 (102-266);Interleukin-33 (109-266);Il33; Il33|9230117N10Rik, Il-, Il-33, Il1f, Il1f11, NF-H, NF-HEV|interleukin 33|interleukin-33|nuclear factor from high endothelial venules
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Il33, check out the Il33 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Il33: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse IL-33 ELISA Kit PicoKine® (EK0930)
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Customer Reviews
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Customer Q&As
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8 Customer Q&As for Mouse IL-33 ELISA Kit PicoKine®
Question
Q: if the enzyme conjugated IL-33 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the IL-33 antigen?
T. Adams
Verified customer
Asked: 2020-11-05
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2020-11-05
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2020-02-09
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can change immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2020-02-09
Question
Q: Can IL-33 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2019-10-22
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is needed. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2019-10-22
Question
Q: how to thaw whole blood sample for IL-33 ELISA after freezing?
K. Dayal
Verified customer
Asked: 2019-08-25
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test IL-33 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2019-08-25
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2019-02-22
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-02-22
Question
Q: What is the optimal O.D. value for IL-33 ELISA kit? I used your IL-33 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain IL-33 even though the O.D. values are not very high?
R. Surve
Verified customer
Asked: 2017-02-06
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your protocol, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this IL-33 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of IL-33 that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2017-02-06
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2016-02-02
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2016-02-02
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement IL-33 using the EK0930 Mouse IL-33 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2015-11-13
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to avoid protein degradation.
Boster Scientific Support
Answered: 2015-11-13
