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Product Info Summary
| SKU: | M01080-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-CD63 Rabbit Monoclonal Antibody
SKU/Catalog Number
M01080-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CD63 Rabbit Monoclonal Antibody catalog # M01080-1. Tested in WB, IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD63 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01080-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
BGG-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CD63
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01080-1 is reactive to CD63 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
55 kDa
Calculated molecular weight
25637 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01080-1 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
IHC 1:500-1:1000
Positive Control
IHC: human spleen tissue
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Western blot analysis of CD63 expression in A375 cell lysate.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunohistochemical analysis of paraffin-embedded human spleen, using CD63 Antibody .
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
Protein Target Info & Infographic
Gene/Protein Information For CD63 (Source: Uniprot.org, NCBI)
Gene Name
CD63
Full Name
CD63 antigen
Weight
25637 MW
Superfamily
tetraspanin (TM4SF) family
Alternative Names
CD63 antigen;Granulophysin;Lysosomal-associated membrane protein 3;LAMP-3;Melanoma-associated antigen ME491;OMA81H;Ocular melanoma-associated antigen;Tetraspanin-30;Tspan-30;CD63;CD63;MLA1, TSPAN30; CD63 LAMP-3, ME491, MLA1, OMA81H, TSPAN30 CD63 molecule CD63 |CD63 (melanoma 1 )|granulophysin|lysosomal-associated membrane protein 3|lysosome-associated membrane glycoprotein 3|melanoma-associated ME491|melanoma-associated MLA1|ocular melanoma-associated |tetraspanin-30|tspan-30
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD63, check out the CD63 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD63: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD63 Rabbit Monoclonal Antibody (M01080-1)
Hello CJ!
M01080-1 has been cited in 4 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Zhu X,Wang S,Tarique I,An T,Yang H,Bai X,Wang X,Chen Q,Yang P.Cellular Evidence and Source of Exosomes in the Biliary System of the Chinese Soft-Shelled Turtle, Pelodiscus sinensis.Front Physiol.2019 Aug 27;10: 1097.doi:10.3389/fphys.2019.01097.PMID:31507456;PMCID: PMC 6718451.
Species: Turtles
M01080-1 usage in article: APP:WB, SAMPLE:BILE,LIVER,GALLBLADDER AND BILIARY EXOSOMES, DILUTION:1:100
Huang C,Pan L,Shen X,Tian H,Guo L,Zhang Z,Liu X.Hsp16.3 of mycobacterium tuberculosis in exosomes as a biomarker of tuberculosis.Eur J Clin Microbiol Infect Dis.2021 Apr 24.doi:10.1007/s10096-021-04246-x.Epub ahead of print.PMID:33893878.
Species: Human
M01080-1 usage in article: APP:WB, SAMPLE:U937 CELL, DILUTION:NA
Cellular Evidence and Source of Exosomes in the Biliary of the Chinese Soft-Shelled Turtle, Pelodiseus Sinensis Xudong Zhu, et al. Front Physiol. 2019; 10: 1097. Published online 2019 Aug 27. doi: 10.3389/fphys.2019.01097
Du Hy, Dong Lh, Zhao Bj, Fu J, Wang Qq, Chen F, Ou L, Li N, Sun X, Tang Zm, Song Hf. Acta Pharmacol Sin. 2012 Aug;33(8):1047-54. Doi: 10.1038/Aps.2012.54. Epub 2012 Jun 25. Immunostimulatory And Anti-Neoplasm Effects Of A Novel Palindrome Cpg Olig...
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Customer Q&As
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4 Customer Q&As for Anti-CD63 Rabbit Monoclonal Antibody
Question
My colleagues were happy with the WB result of your anti-CD63 Rabbit Monoclonal antibody. However we have been able to see positive staining in skeletal muscle cell membrane using this antibody. Is that expected? Could you tell me where is CD63 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-11-12
Answer
Based on literature, skeletal muscle does express CD63. Generally CD63 expresses in cell membrane. Regarding which tissues have CD63 expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 19159218
Lung, and Muscle, Pubmed ID: 15489334
Ovary, Pubmed ID: 2171551
Placenta, Pubmed ID: 17897319
Platelet, Pubmed ID: 7682577
Skeletal muscle, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-11-12
Question
We have been able to see staining in human skin. Any tips? Is anti-CD63 Rabbit Monoclonal antibody supposed to stain skin positively?
Verified Customer
Verified customer
Asked: 2019-10-09
Answer
From what I have seen in literature skin does express CD63. From what I have seen in Uniprot.org, CD63 is expressed in upper lobe of lung, ovary, skin, skeletal muscle, lung muscle, platelet, placenta, liver, among other tissues. Regarding which tissues have CD63 expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 19159218
Lung, and Muscle, Pubmed ID: 15489334
Ovary, Pubmed ID: 2171551
Placenta, Pubmed ID: 17897319
Platelet, Pubmed ID: 7682577
Skeletal muscle, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-10-09
Question
Our lab used your anti-CD63 Rabbit Monoclonal antibody for WB on upper lobe of lung in the past. I am using human, and We want to use the antibody for IHC next. I would like examining upper lobe of lung as well as skeletal muscle in our next experiment. Do you have any suggestion on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2019-05-08
Answer
I have checked the website and datasheets of our anti-CD63 Rabbit Monoclonal antibody and I see that M01080-1 has been validated on human in both WB and IHC. Thus M01080-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-05-08
Question
We are currently using anti-CD63 Rabbit Monoclonal antibody M01080-1 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2017-10-23
Answer
The anti-CD63 Rabbit Monoclonal antibody (M01080-1) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-10-23
