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Product Info Summary
| SKU: | M00597 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-CD26/DPP4 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00597
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CD26/DPP4 Rabbit Monoclonal Antibody catalog # M00597. Tested in WB application. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD26/DPP4 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00597)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ABOC-4
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CD26
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00597 is reactive to DPP4 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
32-42 kDa, 65 kDa, 75 kDa
Calculated molecular weight
88279 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00597 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
Click image to see more details
All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
Click image to see more details
All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
Click image to see more details
All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
Click image to see more details
All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
Click image to see more details
All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
Click image to see more details
Western blot analysis of CD26 expression in HUVEC cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For DPP4 (Source: Uniprot.org, NCBI)
Gene Name
DPP4
Full Name
Dipeptidyl peptidase 4
Weight
88279 MW
Superfamily
peptidase S9B family
Alternative Names
Dipeptidyl peptidase 4;3.4.14.5 ;ADABP;Adenosine deaminase complexing protein 2;ADCP-2;Dipeptidyl peptidase IV;DPP IV;T-cell activation antigen CD26;TP103;CD26;Dipeptidyl peptidase 4 membrane form;Dipeptidyl peptidase IV membrane form;Dipeptidyl peptidase 4 soluble form;Dipeptidyl peptidase IV soluble form;DPP4;ADCP2, CD26; DPP4 ADABP, ADCP2, CD26, DPPIV, TP103 dipeptidyl peptidase 4 dipeptidyl peptidase 4|ADCP-2|DPP IV|Gly-Pro naphthylamidase|Post-proline dipeptidyl aminopeptidase IV|T-cell activation CD26|Xaa-Pro-dipeptidylaminopeptidase|adenosine deaminase complexing protein 2|dipeptidyl peptidase IV|dipeptidylpeptidase 4|dipeptidylpeptidase IV (CD26, adenosine deaminase complexing protein 2)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DPP4, check out the DPP4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DPP4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD26/DPP4 Rabbit Monoclonal Antibody (M00597)
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-CD26/DPP4 Rabbit Monoclonal Antibody
Question
We are currently using anti-CD26 Rabbit Monoclonal antibody M00597 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-12
Answer
The anti-CD26 Rabbit Monoclonal antibody (M00597) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-12
Question
We have observed staining in human prostate uterus. Do you have any suggestions? Is anti-CD26 Rabbit Monoclonal antibody supposed to stain prostate uterus positively?
Verified Customer
Verified customer
Asked: 2019-12-16
Answer
From what I have seen in literature prostate uterus does express DPP4. From what I have seen in Uniprot.org, DPP4 is expressed in tendon of biceps brachii, liver, colon, peripheral blood, placenta, prostate uterus, kidney, plasma, among other tissues. Regarding which tissues have DPP4 expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 1347043, 1977364
Kidney, Pubmed ID: 8096237
Liver, Pubmed ID: 1352704, 19159218, 24275569
Peripheral blood, Pubmed ID: 1352530
Placenta, Pubmed ID: 7927537
Plasma, Pubmed ID: 16335952
Prostate, and Uterus, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-12-16
Question
Our team were well pleased with the WB result of your anti-CD26 Rabbit Monoclonal antibody. However we have been able to see positive staining in plasma dipeptidyl peptidase 4 soluble form: using this antibody. Is that expected? Could you tell me where is DPP4 supposed to be expressed?
K. Kulkarni
Verified customer
Asked: 2018-03-08
Answer
Based on literature, plasma does express DPP4. Generally DPP4 expresses in dipeptidyl peptidase 4 soluble form:, cell membrane. Regarding which tissues have DPP4 expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 1347043, 1977364
Kidney, Pubmed ID: 8096237
Liver, Pubmed ID: 1352704, 19159218, 24275569
Peripheral blood, Pubmed ID: 1352530
Placenta, Pubmed ID: 7927537
Plasma, Pubmed ID: 16335952
Prostate, and Uterus, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2018-03-08
