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Product Info Summary
| SKU: | A06423-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-RASAL1/RASAL1 Antibody Picoband®
SKU/Catalog Number
A06423-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-RASAL1/RASAL1 Antibody Picoband® catalog # A06423-2. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RASAL1/RASAL1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06423-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human RASAL1 recombinant protein (Position: D721-A798).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A06423-2 is reactive to RASAL1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
90 kDa
Calculated molecular weight
90.016kDa
Background of RASAL1
The protein encoded by this gene is member of the GAP1 family of GTPase-activating proteins. And it is mapped to 12q24.13. These proteins stimulate the GTPase activity of normal RAS p21 but not its oncogenic counterpart. Acting as a suppressor of RAS function, the protein enhances the weak intrinsic GTPase activity of RAS proteins resulting in the inactive GDP-bound form of RAS, thereby allowing control of cellular proliferation and differentiation. This particular family member contains domains which are characteristic of the GAP1 subfamily of RasGAP proteins but, in contrast to the other GAP1 family members, this protein is strongly and selectively expressed in endocrine tissues. Alternatively spliced transcript variants that encode different isoforms have been described.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06423-2 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: rat brain tissue, mouse brain tissue, human Raji whole cell,
IHC: rat intestine tissue, mouse spleen tissue, mouse spleen tissue, mouse intestine tissue, mouse brain tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of RASAL1 using anti-RASAL1 antibody (A06423-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates
Lane 2: mouse brain tissue lysates
Lane 3: human Raji whole cell lysates
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RASAL1 antigen affinity purified polyclonal antibody (Catalog # A06423-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RASAL1 at approximately 90KD. The expected band size for RASAL1 is at 90KD.
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Figure 2. IHC analysis of RASAL1 using anti-RASAL1 antibody (A06423-2).
RASAL1 was detected in paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RASAL1 Antibody (A06423-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of RASAL1 using anti-RASAL1 antibody (A06423-2).
RASAL1 was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RASAL1 Antibody (A06423-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of RASAL1 using anti-RASAL1 antibody (A06423-2).
RASAL1 was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RASAL1 Antibody (A06423-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 5. IHC analysis of RASAL1 using anti-RASAL1 antibody (A06423-2).
RASAL1 was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RASAL1 Antibody (A06423-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 6. IHC analysis of RASAL1 using anti-RASAL1 antibody (A06423-2).
RASAL1 was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RASAL1 Antibody (A06423-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For RASAL1 (Source: Uniprot.org, NCBI)
Gene Name
RASAL1
Full Name
RasGAP-activating-like protein 1
Weight
90.016kDa
Alternative Names
RasGAP-activating-like protein 1; RAS protein activator like 1 RASAL1 RASAL RAS protein activator like 1 rasGAP-activating-like protein 1|GAP1 like protein|ras GTPase-activating-like protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RASAL1, check out the RASAL1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RASAL1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RASAL1/RASAL1 Antibody Picoband® (A06423-2)
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Customer Q&As
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4 Customer Q&As for Anti-RASAL1/RASAL1 Antibody Picoband®
Question
Is a blocking peptide available for product anti-RASAL1/RASAL1 antibody (A06423-2)?
Verified Customer
Verified customer
Asked: 2018-03-02
Answer
We do provide the blocking peptide for product anti-RASAL1/RASAL1 antibody (A06423-2). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-03-02
Question
I have attached the WB image, lot number and protocol we used for esophagus mucosa using anti-RASAL1/RASAL1 antibody A06423-2. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-12-07
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-12-07
Question
Does anti-RASAL1/RASAL1 antibody A06423-2 work on horse WB with esophagus mucosa?
S. Krishna
Verified customer
Asked: 2017-03-29
Answer
Our lab technicians have not tested anti-RASAL1/RASAL1 antibody A06423-2 on horse. You can run a BLAST between horse and the immunogen sequence of anti-RASAL1/RASAL1 antibody A06423-2 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse esophagus mucosa in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-03-29
Question
We are currently using anti-RASAL1/RASAL1 antibody A06423-2 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on monkey tissues as well?
W. Carter
Verified customer
Asked: 2015-12-29
Answer
The anti-RASAL1/RASAL1 antibody (A06423-2) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-12-29
