Dipeptidyl peptidase 4 Antibodies

Dipeptidyl peptidase 4 (DPP4)

Cell surface glycoprotein receptor involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Acts as a positive regulator of T-cell coactivation, by binding at ADA, CAV1, IGF2R, and PTPRC.

Its binding to CAV1 and CARD11 induces T-cell proliferation and NF- kappa-B activation in a T-cell receptor/CD3-dependent method. Its interaction with ADA also regulates lymphocyte-epithelial cell adhesion.

Gene Information

Human
Gene Name:	DPP4
Uniprot:	P27487
Entrez:	1803

Family

Belongs to:
peptidase S9B family

Alternative Names

ADABP; ADCP-2; ADCP2DPP IV; Adenosine deaminase complexing protein 2TP103; CD26 antigen; CD26; CD26T-cell activation antigen CD26; dipeptidyl peptidase 4; Dipeptidyl peptidase IV; dipeptidylpeptidase 4; dipeptidyl-peptidase 4; dipeptidylpeptidase IV (CD26, adenosine deaminase complexing protein 2); DPP4; DPPIV; EC 3.4.14.5

Molecular Weight

Mass (kDA):
88.279 kDA

Genomic Context

Human
Location:	2q24.2
Sequence:	2; NC_000002.12 (161992241..162074215, complement)

Tissue Specificity

Expressed specifically in lymphatic vessels but not in blood vessels in the skin, small intestine, esophagus, ovary, breast and prostate glands. Not detected in lymphatic vessels in the lung, kidney, uterus, liver and stomach (at protein level). Expressed in the poorly differentiated crypt cells of the small intestine as well as in the mature villous cells. Expressed at very low levels in the colon.

Subcellular Localizations

[Dipeptidyl peptidase 4 soluble form]: Secreted. Detected in the serum and the seminal fluid.; Cell membrane; Single-pass type II membrane protein. Apical cell membrane; Single-pass type II membrane protein. Cell projection, invadopodium membrane; Single-pass type II membrane protein. Cell projection, lamellipodium membrane; Single-pass type II membrane protein. Cell junction. Membrane raft. Translocated to the apical membrane through the concerted action of N- and O-Glycans and its association with lipid microdomains containing cholesterol and sphingolipids (PubMed:11773049). Redistributed to

Diseases

• Diabetes Mellitus
• Diabetes Mellitus, Non-insulin-dependent
• Hypoglycemia
• Malignant Neoplasms
• Obesity
• Neoplasms
• Insulin Resistance
• Hyperglycemia
• Inflammation
• Carcinoma
• Cardiovascular Diseases
• Malignant Paraganglionic Neoplasm
• Insulin Sensitivity

• Adenomatous Polyposis Coli
• Tissue Adhesions
• Complications Of Diabetes Mellitus
• Diabetes Mellitus, Experimental
• Hypertensive Disease
• Infarction
• Nausea

Interacting Proteins

• CCL11
• CXCL10
• CXCL11
• CXCL2
• CXCL9

• GCG
• VIP

Pathways

• Secretion
• Insulin Secretion
• Glucagon Secretion
• Glucose Homeostasis
• Fibroblast Activation
• Pathogenesis
• Gastric Emptying
• Transport
• Cell Activation
• Localization
• T Cell Activation
• Cell Proliferation
• Regeneration

• Excretion
• Cell Migration
• Angiogenesis
• Cell Adhesion
• Glycosylation
• Immune Response
• Glomerular Filtration

PTMs

• Cleavage
• Glycosylation
• Phosphorylation
• Amidation
• Acetylation

• Methylation
• Oxidation
• Acylation
• Gpi Anchoring
• Ubiquitination

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/DPP4

Boster Bio Anti-CD26 DPP4 Marker and Human CD26/DPP4 ELISA Kit

This article explores the Boster Bio Anti-CD26 DPP4 Marker and the Human CD26/DPP4 ELISA kit. We also explore the benefits and tips for optimizing the DPP4 marker. You can improve your experiments and achieve better results by following the Boster Bio optimization guide. It is possible to eliminate the causes of error in your experiments using the right controls.

Boster Bio Anti-CD26 DPP4 Marker

Designed for use in flow cytometry, immunohistochemistry, ICC, and IHC applications, Boster Bio Anti-CD26 DPP2 Polyclonal Antibody reacts with Human, Mouse, and Rat. Its high affinity and selective nature make it an excellent choice for a range of cell-based applications. The product is appropriate for researchers from all disciplines.

The study also found that the anti-CD26 DPP4 isotypes performed better in adenomas that are more advanced, indicating that these biomarkers can improve the diagnosis of various conditions. Anti-CD26 isotypes are able detect a wider range of cell types than the sCD26. In turn, they can be used in combination with sCD26 in more advanced cancer cases.

In an ongoing cohort study, 497 subjects were tested for the presence of biomarkers for colon cancer. A subset of patients were subjected to the faecal occult faecal blood (FIT) test. The colonoscopy results of subjects who had FIT were used to study improvements in sCD26 as a biomarker of CRC.

Human CD26/DPP4 ELISA Kit

The Boster Bio Human CD26/DPP4. ELISA kit is built on the sandwich ELISA technology. This kit contains two antibodies, the monoclonal mouse antibody as well as an biotinylated polyclonal detection protein goat antibody as well as an TMB substrate. The matched antibodies recognize Hu CD26 within plasma, serum, and saliva. The results are reported as mean results of duplicate measurements as well as an sigmoid-shaped standard-curve.

CD26 is a multifunctional cell surface glycoprotein with dipeptidyl peptidase similar activity. It is present in plasma in soluble form and has a nebulous function. It is however well-known to play a key role in the biology of cancer. Serum CD26 levels were decreased in melanoma and colon cancer, suggesting that this gene might be involved in the development of cancer.

The human CD26/DPP4 ELISA kit has an sensitivity of 10pg/ml in both urine and serum. The kit comes with 96 wells that have removable strips. The kit includes all the essential components for conducting the assay. The kit is easy to use. Follow the instructions provided with the kit. It is a reliable and inexpensive way to detect Human CD26 in samples.

The Boster Bio Human CD26/DPP4" ELISA kit can measure the presence of CD26/DPP4 within biological samples. It detects native DPP4 by using the DPP4 antibody. It is also validated to detect DPP4 in a quantitative manner. antigen. If your research involves the detection of human CD26/DPP4 using this kit, it is possible to compare it to other tests and determine whether there is any difference.

CD26 is a crucial diagnostic marker for gastric cancer. It has increased sensitivity compared to other biomarkers. Serum CD26 levels are significantly lower for patients with gastric cancer compared to healthy donors. However, the CD26 levels did not differ significantly between men and women. Incredibly, the CD26 levels could be an early indicator of gastric cancer. It can also be used to determine the presence of a cancerous tumor.

The DPP4 Marker comes with many benefits

There are many clinical trials in the pipeline that will investigate the benefits of using the DPP4 marker for diabetes. The DPP4 enzyme is a key player in the process of epithelial-mesenchymal transition, which occurs in ESRD patients. This mechanism could affect kidney functions, including glomerularfiltration rate (GFR) and blood flow. In clinical trials conducted recently, DPP4 inhibition has been proven to reduce the glucose-induced elevation of the DPP4 marker. DPP4 inhibition is being investigated in different research studies to determine its impact on the flow of blood through the kidney, vascular stiffness , and systemic inflammation.

Standard methods were employed to assess DPP4 activity. DPP4 activity was significantly increased in serum, peritoneal fluids and F344 PF in line with other studies. This was a positive result that established a positive correlation between DPP4 activity and peritoneal injury. The DPP4 marker is used in diabetes, in turn will allow us to detect and monitor diabetes earlier.

Tips to optimize your business

There are a myriad of options when it comes to ELISA protocols. How to prepare the samples, how to concentrate the antibodies, and what buffers to employ are few examples. Boster Bio optimization techniques are available to help you optimize your research. Find answers to these questions and much more on our technical blog. This article will explain the fundamentals of the protocols and principles of ELISA and the specific products you will require.