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Product Info Summary
| SKU: | EK1127 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human CCL16/HCC-4 ELISA Kit PicoKine®
View all CCL16/HCC-4/LEC ELISA kits
SKU/Catalog Number
EK1127
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human CCL16/HCC-4 ELISA Kit PicoKine® (96 Tests). Quantitate Human CCL16 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human CCL16/HCC-4 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1127)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: Q24-Q120
Sensitivity
<2 pg/ml
Assay Range
7.8 pg/ml - 500 pg/ml
Standard Dilution Instructions
See datasheet of EK1127 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1127 is reactive to CCL16 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK1127 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CCL16/HCC-4/LEC
Chemokine (C-C motif) ligand 16 (CCL16) is a small cytokine belonging to the CC chemokine family that is known under several pseudonyms, including Liver-expressed chemokine (LEC) and Monotactin-1 (MTN-1). This chemokine is expressed by the liver, thymus, and spleen and is chemoattractive for monocytes and lymphocytes. Cellular expression of CCL16 can be strongly induced in monocytes by IL-10, IFN-gamma and bacterial lipopolysaccharide. Its gene is located on chromosome 17, in humans, among a cluster of other CC chemokines. CCL16 elicits its effects on cells by interacting with cell surface chemokine receptors such as CCR1, CCR2, CCR5 and CCR8.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1127-CAP | Anti-Human CCL16 Pre-coated 96-well strip microplate | 1 |
| EK1127-ST | Human CCL16 Standard | 2 vials, 10 ng/tube |
| EK1127-DA | Human CCL16 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 7.8 | 15.6 | 31.3 | 62.5 | 125 | 250 | 500 |
| O.D. | 0.024 | 0.070 | 0.117 | 0.200 | 0.384 | 0.777 | 1.422 | 2.095 |
Data Example Images
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Human CCL16/HCC-4 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human CCL16/HCC-4 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 17 | 60 | 271 | 18 | 54 | 267 |
| Standard deviation | 0.85 | 3.18 | 20.86 | 1.02 | 3.56 | 21.36 |
| CV (%) | 5% | 5.3% | 7.7% | 5.7% | 6.6% | 8% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of CCL16/HCC-4/LEC in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 17 | 16 | 17 | 17 | 16 | 0.43 | 2.6% |
| Sample 2 | 60 | 53 | 52 | 58 | 55 | 3.34 | 6% |
| Sample 3 | 271 | 227 | 243 | 230 | 242 | 17.38 | 7.1% |
Protein Target Info & Infographic
Gene/Protein Information For CCL16 (Source: Uniprot.Org, NCBI)
Gene Name
CCL16
Full Name
C-C motif chemokine 16
Weight
13600 MW
Superfamily
intercrine beta (chemokine CC) family
Alternative Names
C-C motif chemokine 16;Chemokine CC-4;HCC-4;Chemokine LEC;IL-10-inducible chemokine;LCC-1;Liver-expressed chemokine;Lymphocyte and monocyte chemoattractant;LMC;Monotactin-1;MTN-1;NCC-4;Small-inducible cytokine A16;CCL16;ILINCK, NCC4, SCYA16; CCL16 CKb12, HCC-4, ILINCK, LCC-1, LEC, LMC, Mtn-1, NCC-4, NCC4, SCYA16, SCYL4 C-C motif chemokine ligand 16 C-C motif chemokine 16|IL-10-inducible chemokine|chemokine (C-C motif) ligand 16|chemokine LEC|liver CC chemokine-1|liver-expressed chemokine|lymphocyte and monocyte chemoattractant|monotactin-1|new CC chemokine 4|small inducible cytokine subfamily A (Cys-Cys), member 16|small-inducible cytokine A16
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CCL16, check out the CCL16 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CCL16: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human CCL16/HCC-4 ELISA Kit PicoKine® (EK1127)
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Customer Q&As
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6 Customer Q&As for Human CCL16/HCC-4 ELISA Kit PicoKine®
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring CCL16 using the EK1127 Human CCL16 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2020-11-05
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to avoid protein degradation.
Boster Scientific Support
Answered: 2020-11-05
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
W. Dhawan
Verified customer
Asked: 2020-02-21
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2020-02-21
Question
Q: we need your recommendation regarding the dilution ratio of serum samples for detection of CCL16 in Human serum? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.
J. Williams
Verified customer
Asked: 2020-01-05
Answer
A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the CCL16 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2020-01-05
Question
Q: can I use citrate plasma as samples in Human CCL16 Picokine® ELISA Kit (Catalog # EK1127)?
W. Li
Verified customer
Asked: 2019-08-07
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of CCL16 causing degradation of its protein structure. CCL16 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the CCL16 ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2019-08-07
Question
Q: how do I analyze ELISA data? I measured CCL16 level in serum.
M. Harris
Verified customer
Asked: 2017-09-29
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2017-09-29
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2016-01-18
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2016-01-18
