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Product Info Summary
| SKU: | EK0307 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA, citrate). |
Product info
Product Name
Human BDNF ELISA Kit PicoKine®
SKU/Catalog Number
EK0307
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human BDNF ELISA Kit PicoKine® (96 Tests). Quantitate Human BDNF in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA, citrate). Sensitivity: 15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human BDNF ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0307)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: H129-R247
Sensitivity
<15 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0307 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0307 is reactive to BDNF in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA, citrate).
Application Guarantee
EK0307 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Bdnf
Brain-derived neurotrophic factor (BDNF) is a prosurvival factor induced by cortical neurons that is necessary for survival of striatal neurons in the brain. It is a secreted protein with the molecular weight of 27.8kDa, consisting of 247 amino acids. It is known to promote neuronal survival and differentiation. BDNF shares substantial amino acid sequence identity with nerve growth factor (NGF). BDNF and neurotrophin-3 (NT-3) are two recently cloned neurotrophic factors that are homologous to NGF. mRNA products of the BDNF and NT-3 genes are detected in the adult human brain, suggesting that these proteins are involved in the maintenance of the adult nervous system. BDNF and other neurotrophins are critically involved in long-term potentiation (LTP). BDNF-mediated LTP is induced postsynaptically. BDNF has trophic effects on serotonergic (5-HT) neurons in the central nervous system. BDNF has an essential maintenance function in the regulation of anxiety-related behavior and in food intake through central mediators in both the basal and fasted state. It plays a role in treating breathing disorders such as respiratory insufficiency after spinal injury. The mature form of BDNF is identical in all mammals examined, and the gene encoding human BDNF to chromosome 11, band p13.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0307-CAP | Anti-Human BDNF Pre-coated 96-well strip microplate | 1 |
| EK0307-ST | Human BDNF Standard | 2 vials, 10 ng/tube |
| EK0307-DA | Human BDNF Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.025 | 0.074 | 0.101 | 0.160 | 0.287 | 0.563 | 1.251 | 1.916 |
Data Example Images
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Human BDNF PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Bdnf, For serum, dilution ratio is 1:100 and concentration is 7233-69396 pg/ml.
For plasma, dilution ratio is 1:1 and concentration is 80-2694 pg/ml..
Some articles we found to cite concentrations of Bdnf in samples: 24812918 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human BDNF ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 47 | 224 | 1022 | 44 | 244 | 926 |
| Standard deviation | 3.57 | 9.63 | 44.96 | 3.78 | 12.44 | 50 |
| CV (%) | 7.6% | 4.3% | 4.4% | 8.6% | 5.1% | 5.4% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Bdnf in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 47 | 56 | 51 | 52 | 51 | 3.2 | 6.2% |
| Sample 2 | 224 | 188 | 221 | 204 | 209 | 14.44 | 6.9% |
| Sample 3 | 1022 | 1010 | 870 | 1050 | 988 | 69.65 | 7% |
Protein Target Info & Infographic
Gene/Protein Information For BDNF (Source: Uniprot.Org, NCBI)
Gene Name
BDNF
Full Name
Brain-derived neurotrophic factor
Weight
27818 MW
Superfamily
NGF-beta family
Alternative Names
Brain-derived neurotrophic factor;BDNF;Abrineurin;BDNF; BDNF ANON2, BULN2 brain derived neurotrophic factor brain-derived neurotrophic factor|abrineurin|neurotrophin
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on BDNF, check out the BDNF Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for BDNF: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human BDNF ELISA Kit PicoKine® (EK0307)
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Customer Reviews
Have you used Human BDNF ELISA Kit PicoKine®?
Submit a review and receive an Amazon gift card.
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1 Reviews For Human BDNF ELISA Kit PicoKine®
0
Quantification of BDNF Post-Transfection in Primary Neuronal Cells--Sanjay Arora, North Dakota State University, Pharmaceutical Sciences, Graduate Student
Excellent
Source: Biocompare.com
| Application | ELISA |
|---|---|
| Sample | Cell lysate and culture medium |
| Detection | -- |
"The kit was used to quantify amount of BDNF produced after transfection with plasmid DNA encoding human BDNF using non viral vector, liposomes. Provided good reliable quantification of BDNF produced. No interference. Good reproducibility. Reliable estimation, good sensitivity. Reasonable price as well. Would purchase again." (Customer’s Tip: "Don't dilute sample, assay directly.")
Sanjay Arora
Verified customer
Submitted 2019-05-21
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
13 Customer Q&As for Human BDNF ELISA Kit PicoKine®
Question
What isoform does EK0307 detect?
Verified customer
Asked: 2020-11-16
Answer
The Human BDNF ELISA Kit PicoKine™ (EK0307) detects total BDNF.
Boster Scientific Support
Answered: 2020-11-16
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification Bdnf using the EK0307 Human Bdnf Picokine® ELISA Kit?
B. Gandhi
Verified customer
Asked: 2020-04-03
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to avoid protein degradation.
Boster Scientific Support
Answered: 2020-04-03
Question
Will this ELISA kit work on saliva or nasal washes?
Verified Customer
Verified customer
Asked: 2020-03-06
Answer
We have not validated this kit on saliva or nasal washes.It can be detected if the concentration of BDNF in saliva or nasal wash samples are in the detection range of the kit.
Boster Scientific Support
Answered: 2020-03-06
Question
My samples have highly concentrated levels of BDNF and require more than normal dilution. Can you provide any extra (~ 90 mL) sample diluent? Do you sell the diluent separately?
Verified Customer
Verified customer
Asked: 2020-02-12
Answer
Yes, we sell the diluent separately (Boster Catalog# AR1106-1). In fact, the sample diluent buffer in the kit is generally enough. For instance, if the dilution ratio is 1:20000, it is not necessary to dilute the samples at 1:20000 in just one step. Instead, we suggest you sequentially dilute the samples: 1:100, 1.200, etc. That way, you can use less diluent buffer.
Boster Scientific Support
Answered: 2020-02-12
Question
Which isoform (mature or precursor) does this ELISA assay detect? I am also wondering if the dimer form of mature BDNF can be detection by ELISA.
Verified Customer
Verified customer
Asked: 2019-12-23
Answer
The assay recognizes both of mature and precursor isoforms of BDNF and can detect the dimer form. So, this detects total BDNF.
Boster Scientific Support
Answered: 2019-12-23
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2019-09-17
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2019-09-17
Question
I am interested in performing Western Blot (WB) using the antibodies from your human and mouse BDNF ELISA kits. Is it possible to order the primary antibody (the capture antibody) that is pre-coated in the wells for the sandwich ELISA? (In this case that would be the monoclonal antibody from rat for the mouse kit, and the monoclonal antibody from mouse for the human kit). Is it also possible to order your secondary antibody which is the biotinylated detection polyclonal antibody from goat specific for BDNF?
Verified Customer
Verified customer
Asked: 2019-08-28
Answer
The antibodies used in those ELISA kits are not fully validated on WB. Also, since these antibodies are made from recombinant proteins, they are often less reactive to retrieved antigens in WB or IHC due to the lack of tertiary structure in the retrieved antigens. If you want to test human and mouse BDNF with WB, please use our anti-BDNF antibody (PB9075). Further, you can separately buy our secondary antibodies.
Boster Scientific Support
Answered: 2019-08-28
Question
Is the BDNF measured by this Human BDNF ELISA Kit PicoKine a mature BDNF or pro-BDNF? What is the conversion factor to calculate the concentration in moles?
Verified Customer
Verified customer
Asked: 2019-06-21
Answer
The capture and detection used in this Human BDNF ELISA Kit PicoKine antibodies are both recognizes the region within Arg128
Boster Scientific Support
Answered: 2019-06-21
Question
Hello Support: I would like to ask if this antibody Human BDNF ELISA Kit PicoKine would quantify and detect BDNF in cerebrospinal fluid, not only in plasma or cell culture.
Verified Customer
Verified customer
Asked: 2019-06-14
Answer
The product Human BDNF ELISA Kit PicoKine has not been tested in cerebrospinal fluid, it is only validated in with plasma, serum and cell culture, however, this kit is very accommodating to many different samples types, so cerebrospinal fluid will most likely work
Boster Scientific Support
Answered: 2019-06-14
Question
Q: Can Bdnf ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
T. Puri
Verified customer
Asked: 2019-01-24
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2019-01-24
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
M. Jones
Verified customer
Asked: 2016-03-20
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2016-03-20
Question
What is the expression system for the standards? Do the standards contain carrier protein? Keywords: product immunogen information, antigen sequence, expression standard
Verified Customer
Verified customer
Asked: 2014-04-28
Answer
The expression system of the standards can be found in the product page under "
Boster Scientific Support
Answered: 2014-04-28
Question
Does EK0307 have any cross-reactivity with other neurotrophins, especially proBDNF?
Verified Customer
Verified customer
Asked: 2014-03-12
Answer
EK0307 is able to detect total BDNF. It has no significant cross-reactivity or interference with the following factors: BDNF (pro-domain, aa 19-128), NGF-beta, NGFR, NT-3, NT-4, TrkA, and TrkB.
Boster Scientific Support
Answered: 2014-03-12
