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Product Info Summary
| SKU: | PA1318 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-c-Fos Antibody
SKU/Catalog Number
PA1318
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-c-Fos Antibody catalog # PA1318. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-c-Fos Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1318)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human c-Fos, identical to the related rat and mouse sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1318 is reactive to FOS in Human, Mouse, Rat
Applications
PA1318 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
37 kDa
Calculated molecular weight
40695 MW
Background of c-Fos
The human oncogene c-fos is cellular homolog of the transforming gene of Finkel-Biskis-Jinkins (FBJ) murine osteosarcoma virus which was mapped to a single human chromosome. c-Fos is encoded by the FOS gene. FOS was the first transcription factor identified that has a critical function in regulating the development of cells destined to form and maintain the skeleton. FOS is also a major component of the activator protein-1 (AP-1) transcription factor complex, which includes members of the JUN family. c-fos is a major nuclear target for signal transduction pathways involved in the regulation of cell growth, differentiation, and transformation. Using transgenic and knockout mice, Grigoriadis et al. (1995) established a unique role for the proto-oncogene and nuclear transcription factor, Fos, in regulating the differentiation and activity of specific bone cell populations, both during normal development and in bone disease.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Validation Images & Assay Conditions
Click image to see more details
Anti-c-Fos antibody, PA1318, Western blotting
Lane 1: HT1080 Cell Lysate
Lane 2: COLO320 Cell Lysate
Protein Target Info & Infographic
Gene/Protein Information For FOS (Source: Uniprot.org, NCBI)
Gene Name
FOS
Full Name
Proto-oncogene c-Fos
Weight
40695 MW
Superfamily
bZIP family
Alternative Names
activator protein 1; AP-1; cellular oncogene c-fos; Cellular oncogene fos; cFos; c-Fos; FBJ murine osteosarcoma viral (v-fos) oncogene homolog (oncogene FOS); FBJ murine osteosarcoma viral oncogene homolog; FOS; G0/G1 switch regulatory protein 7; G0S7; proto-oncogene c-Fos; v-fos FBJ murine osteosarcoma viral oncogene homolog FOS AP-1, C-FOS, p55 Fos proto-oncogene, AP-1 transcription factor subunit proto-oncogene c-Fos|FBJ murine osteosarcoma viral (v-fos) oncogene homolog (oncogene FOS)|FBJ murine osteosarcoma viral oncogene homolog|Fos proto-oncogene, AP-1 trancription factor subunit|G0/G1 switch regulatory protein 7|activator protein 1|cellular oncogene c-fos
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FOS, check out the FOS Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FOS: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-c-Fos Antibody (PA1318)
Hello CJ!
PA1318 has been cited in 17 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
NMDA Receptor Signaling Mediates cFos Expression via Top2β-Induced DSBs in Glioblastoma Cells
Overexpression of c-fos in Helicobacter pylori-induced gastric precancerosis of Mongolian gerbil
Changes in the expression of c-fos & heat shock protein genes & blood flow velocity in the brain of rats undergoing myocardial ischaemia/reperfusion
Tumor suppressive role of miR-569 in lung cancer
Positional and expressive alteration of prohibitin during the induced differentiation of human hepatocarcinoma SMMC-7721 cells
Analgesic Neural Circuits Are Activated by Electroacupuncture at Two Sets of Acupoints
miR-144 and targets, c-fos and cyclooxygenase-2 (COX2), modulate synthesis of PGE2 in the amnion during pregnancy and labor
The Expression Patterns of c-Fos and c-Jun Induced by Different Frequencies of Electroacupuncture in the Brain
Neurotoxicity of intrathecal injections of dexmedetomidine into the rat spinal dorsal horn
Liu W, Peng Qx, Lin Xl, Luo Ch, Jiang Mj, Mo Zx, Yung Kk. Fitoterapia. 2014 Jan;92:16-22. Doi: 10.1016/J.Fitote.2013.10.002. Epub 2013 Oct 17. Effect Of Rhynchophylline On The Expression Of P-Creb And Sc-Fos In Triatum And Hippocampal Ca1 Area Of ...
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Customer Q&As
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16 Customer Q&As for Anti-c-Fos Antibody
Question
I have a question about product PA1318, anti-c-Fos antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-02-28
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1318 anti-c-Fos antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-02-28
Question
We are interested in using your anti-c-Fos antibody for transforming growth factor beta receptor signaling pathway studies. Has this antibody been tested with western blotting on ht1080 cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-02-26
Answer
We appreciate your inquiry. This PA1318 anti-c-Fos antibody is validated on ht1080 cell lysate, colo320 cell lysate. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-02-26
Question
Do you have a BSA free version of anti-c-Fos antibody PA1318 available?
Verified Customer
Verified customer
Asked: 2020-01-22
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-c-Fos antibody PA1318 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-01-22
Question
Please see the WB image, lot number and protocol we used for leukemic t-cell using anti-c-Fos antibody PA1318. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-12-12
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-12
Question
Would anti-c-Fos antibody PA1318 work on pig WB with colon?
Verified Customer
Verified customer
Asked: 2019-07-22
Answer
Our lab technicians have not tested anti-c-Fos antibody PA1318 on pig. You can run a BLAST between pig and the immunogen sequence of anti-c-Fos antibody PA1318 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig colon in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-22
Question
I see that the anti-c-Fos antibody PA1318 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-06-25
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-06-25
Question
Does anti-c-Fos antibody PA1318 work for WB with leukemic t-cell?
Verified Customer
Verified customer
Asked: 2019-06-11
Answer
According to the expression profile of leukemic t-cell, FOS is highly expressed in leukemic t-cell. So, it is likely that anti-c-Fos antibody PA1318 will work for WB with leukemic t-cell.
Boster Scientific Support
Answered: 2019-06-11
Question
I am interested in to test anti-c-Fos antibody PA1318 on mouse leukemic t-cell for research purposes, then I may be interested in using anti-c-Fos antibody PA1318 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-06-07
Answer
The products we sell, including anti-c-Fos antibody PA1318, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-06-07
Question
Will PA1318 anti-c-Fos antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-10-05
Answer
You can see on the product datasheet, PA1318 anti-c-Fos antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-10-05
Question
Is a blocking peptide available for product anti-c-Fos antibody (PA1318)?
Verified Customer
Verified customer
Asked: 2017-12-27
Answer
We do provide the blocking peptide for product anti-c-Fos antibody (PA1318). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-12-27
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukemic t-cell using anti-c-Fos antibody PA1318. Let me know if you need anything else.
P. Brown
Verified customer
Asked: 2017-09-21
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-09-21
Question
Is this PA1318 anti-c-Fos antibody reactive to the isotypes of FOS?
Verified Customer
Verified customer
Asked: 2017-06-13
Answer
The immunogen of PA1318 anti-c-Fos antibody is A synthetic peptide corresponding to a sequence in the middle region of human c-Fos(170-187aa DQLEDEKSALQTEIANLL), identical to the related rat and mouse sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-06-13
Question
I was wanting to use your anti-c-Fos antibody for WB for mouse leukemic t-cell on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse leukemic t-cell identification?
N. Thomas
Verified customer
Asked: 2016-01-25
Answer
It shows on the product datasheet, PA1318 anti-c-Fos antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse leukemic t-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-01-25
Question
We have observed staining in rat mucosa of stomach. Any tips? Is anti-c-Fos antibody supposed to stain mucosa of stomach positively?
W. Li
Verified customer
Asked: 2014-10-09
Answer
From what I have seen in literature mucosa of stomach does express FOS. From what I have seen in Uniprot.org, FOS is expressed in mucosa of stomach, colon, pancreas, leukemic t-cell, among other tissues. Regarding which tissues have FOS expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 14702039
Leukemic T-cell, Pubmed ID: 19690332
Pancreas, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2014-10-09
Question
My colleagues were happy with the WB result of your anti-c-Fos antibody. However we have observed positive staining in leukemic t-cell nucleus. endoplasmic reticulum. cytoplasm, using this antibody. Is that expected? Could you tell me where is FOS supposed to be expressed?
A. Evans
Verified customer
Asked: 2013-12-25
Answer
According to literature, leukemic t-cell does express FOS. Generally FOS expresses in nucleus. endoplasmic reticulum. cytoplasm,. Regarding which tissues have FOS expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 14702039
Leukemic T-cell, Pubmed ID: 19690332
Pancreas, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2013-12-25
Question
We are currently using anti-c-Fos antibody PA1318 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on bovine tissues as well?
Z. Roberts
Verified customer
Asked: 2013-03-27
Answer
The anti-c-Fos antibody (PA1318) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-03-27
