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Product Info Summary
| SKU: | A03019 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-MAG Antibody Picoband®
View all MAG/Siglec-4a Antibodies
SKU/Catalog Number
A03019
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MAG Antibody Picoband® catalog # A03019. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MAG Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03019)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human MAG recombinant protein (Position: E34-R605).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03019 is reactive to MAG in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
100 kDa
Calculated molecular weight
69.069kDa
Background of MAG/Siglec-4a
MAG (Myelin-associated glycoprotein),also known as SIGLEC4A, is a cell membrane glycoprotein that is a member of the SIGLEC family of proteins and is a functional ligand of the NOGO-66 receptor, NgR. It is though to be involved in the process of myelination. MAG is a sialic acid-binding SIGLEC protein and is a functional ligand for the NOGO receptor.The MAG gene is mapped on 19q13.12. Cleavage of GPI-linked proteins from axons protects growth cones from MAG-induced collapse, and dominant-negative NgR eliminates MAG inhibition of neurite outgrowth. MAG-resistant embryonic neurons were rendered MAG-sensitive by expression of NgR. MAG binds specifically to an NgR-expressing cell line in a GPI-dependent and sialic acid-independent manner. Experiments blocking NgR from interacting with MAG prevented inhibition of neurite outgrowth by MAG. In cultured human embryonic kidney (HEK) cells expressing the NOGO receptor, p75 (NTR) was required for MAG-induced intracellular calcium elevation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03019 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25μg/ml, Mouse, Rat
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: rat brain tissue, mouse brain tissue
FCM: PC-3 cell, K562 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of MAG using anti-MAG antibody (A03019).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAG antigen affinity purified polyclonal antibody (Catalog # A03019) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAG at approximately 100 kDa. The expected band size for MAG is at 69 kDa.
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Figure 2. Flow Cytometry analysis of PC-3 cells using anti-MAG antibody (A03019).
Overlay histogram showing PC-3 cells stained with A03019 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MAG Antibody (A03019, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Figure 3. Flow Cytometry analysis of K562 cells using anti-MAG antibody (A03019).
Overlay histogram showing K562 cells stained with A03019 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-MAG Antibody (A03019, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For MAG (Source: Uniprot.org, NCBI)
Gene Name
MAG
Full Name
Myelin-associated glycoprotein
Weight
69.069kDa
Superfamily
immunoglobulin superfamily
Alternative Names
Myelin-associated glycoprotein; Siglec-4a; MAG; GMA MAG GMA, S-MAG, SIGLEC-4A, SIGLEC4A, SPG75 myelin associated glycoprotein myelin-associated glycoprotein|sialic acid binding Ig-like lectin 4A|sialic acid-binding immunoglobulin-like lectin 4A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MAG, check out the MAG Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MAG: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MAG Antibody Picoband® (A03019)
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Customer Q&As
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5 Customer Q&As for Anti-MAG Antibody Picoband®
Question
We have seen staining in human c1 segment of cervical spinal cord. Are there any suggestions? Is anti-MAG antibody supposed to stain c1 segment of cervical spinal cord positively?
Verified Customer
Verified customer
Asked: 2020-04-01
Answer
From literature c1 segment of cervical spinal cord does express MAG. From Uniprot.org, MAG is expressed in c1 segment of cervical spinal cord, brain, brain eco:0000312, embl:caa67055.1, among other tissues. Regarding which tissues have MAG expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 2479762, 9495552, 14702039, 15489334
Boster Scientific Support
Answered: 2020-04-01
Question
We have tried in the past anti-MAG antibody for ELISA on embl:caa67055.1} in a previous project. I am using rat, and We want to use the antibody for Flow Cytometry next. We want examining embl:caa67055.1} as well as brain eco:0000312 in our next experiment. Do you have any suggestion on which antibody would work the best for Flow Cytometry?
Verified Customer
Verified customer
Asked: 2018-05-18
Answer
I have checked the website and datasheets of our anti-MAG antibody and I see that A03019 has been tested on rat in both ELISA and Flow Cytometry. Thus A03019 should work for your application. Our Boster satisfaction guarantee will cover this product for Flow Cytometry in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for Flow Cytometry detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-05-18
Question
Our team were content with the WB result of your anti-MAG antibody. However we have been able to see positive staining in c1 segment of cervical spinal cord cell membrane using this antibody. Is that expected? Could you tell me where is MAG supposed to be expressed?
Verified Customer
Verified customer
Asked: 2017-11-14
Answer
According to literature, c1 segment of cervical spinal cord does express MAG. Generally MAG expresses in cell membrane. Regarding which tissues have MAG expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 2479762, 9495552, 14702039, 15489334
Boster Scientific Support
Answered: 2017-11-14
Question
We are currently using anti-MAG antibody A03019 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on feline tissues as well?
S. Baker
Verified customer
Asked: 2015-01-19
Answer
The anti-MAG antibody (A03019) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-01-19
Question
I was wanting to use using your anti-MAG antibody for transmission of nerve impulse studies. Has this antibody been tested with western blotting on siha cells? We would like to see some validation images before ordering.
R. Williams
Verified customer
Asked: 2014-04-02
Answer
Thanks for your inquiry. This A03019 anti-MAG antibody is validated on rat brain tissue, mouse brain, siha cells. It is guaranteed to work for ELISA, Flow Cytometry, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2014-04-02
