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Product Info Summary
| SKU: | M00297-4 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-C-FOS Antibody
SKU/Catalog Number
M00297-4
Size
100ul
Description
Boster Bio Anti-C-FOS Antibody catalog # M00297-4. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-C-FOS Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00297-4)
Host
Rabbit
Contents
Purified antibody at 1mg/mL in 50% PBS, 50% glycerol plus 5mM NaN3
Clonality
Polyclonal
Immunogen
Full length recombinant human protein expressed in and purified from E. coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00297-4 is reactive to FOS in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
40695 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00297-4 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2,000
IF/ICC: 1:2,000
IHC: 1:5,000-10,000
Validation Images & Assay Conditions
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Western blot analysis of cell lysates using rabbit pAb to c-FOS, M00297-4, dilution 1:2,000, in green, and mouse mAb to GAPDH, dilution 1:5,000, in red, used as a loading control. [1] protein standard (red), [2] HeLa cells grown in FBS free media, [3] HeLa cells stimulated with 20% FBS for 2 hours after being in FBS free media for 36 hours, [4] rat cortical neurons, [5] rat cortical neurons treated with membrane depolarization buffer for 5 hours. Multiple bands at 50-65 kDa in stimulated or treated cell lysates, correspond to different isoforms of the c-FOS protein.
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Immunofluorescent analysis of HeLa cells stained with rabbit pAb to c-FOS, M00297-4, dilution 1:2,000 in red, and mouse mAb to tubulin, dilution 1:10,000, in green. The blue is DAPI staining of nuclear DNA. HeLa cells were kept in fetal bovine serum (FBS) – free media for 36 hours. Then the cells were treated with PBS (A), as a control, or stimulated with 20% FBS (B) for 30 min. c-FOS antibody labels only the nuclei of stimulated cells. Mouse hippocampus (C) or olfactory bulb sections (D) stained with M00297-4, dilution 1:10,000 in red, and mouse mAb to NF-L, dilution 1:5,000, in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of mouse with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45M, and free-floating sections were stained with above antibodies. The c-FOS antibody stains only nuclei of spontaneously active neurons. NF-L is expressed in axons of neuronal cells.
Protein Target Info & Infographic
Gene/Protein Information For FOS (Source: Uniprot.org, NCBI)
Gene Name
FOS
Full Name
Proto-oncogene c-Fos
Weight
40695 MW
Superfamily
bZIP family
Alternative Names
Proto-oncogene c-Fos;Cellular oncogene fos;G0/G1 switch regulatory protein 7;FOS;G0S7; FOS AP-1, C-FOS, p55 Fos proto-oncogene, AP-1 transcription factor subunit proto-oncogene c-Fos|FBJ murine osteosarcoma viral (v-fos) oncogene homolog (oncogene FOS)|FBJ murine osteosarcoma viral oncogene homolog|Fos proto-oncogene, AP-1 trancription factor subunit|G0/G1 switch regulatory protein 7|activator protein 1|cellular oncogene c-fos
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FOS, check out the FOS Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FOS: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-C-FOS Antibody (M00297-4)
Hello CJ!
M00297-4 has been cited in 6 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Neurotoxic effect of chronic heroin administration on the expression of c-Fos and Bax and glial cells in rat prefrontal cortex
Overexpression of c-fos in Helicobacter pylori-induced gastric precancerosis of Mongolian gerbil
Changes in the expression of c-fos & heat shock protein genes & blood flow velocity in the brain of rats undergoing myocardial ischaemia/reperfusion
Analgesic Neural Circuits Are Activated by Electroacupuncture at Two Sets of Acupoints
miR-144 and targets, c-fos and cyclooxygenase-2 (COX2), modulate synthesis of PGE2 in the amnion during pregnancy and labor
The Expression Patterns of c-Fos and c-Jun Induced by Different Frequencies of Electroacupuncture in the Brain
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-C-FOS Antibody
Question
My question regarding product M00297-4, anti-C-FOS antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-03-04
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00297-4 anti-C-FOS antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-03-04
Question
Is a blocking peptide available for product anti-C-FOS antibody (M00297-4)?
Verified Customer
Verified customer
Asked: 2020-03-03
Answer
We do provide the blocking peptide for product anti-C-FOS antibody (M00297-4). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-03-03
Question
Do you have a BSA free version of anti-C-FOS antibody M00297-4 available?
Verified Customer
Verified customer
Asked: 2019-12-10
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-C-FOS antibody M00297-4 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-12-10
Question
We bought anti-C-FOS antibody for IHC on colon last year. I am using rat, and We are going to use the antibody for WB next. you antibody examining colon as well as pancreas in our next experiment. Could give a recommendation on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2019-11-19
Answer
I viewed the website and datasheets of our anti-C-FOS antibody and it seems that M00297-4 has been tested on rat in both IHC and WB. Thus M00297-4 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-11-19
Question
Is this M00297-4 anti-C-FOS antibody reactive to the isotypes of FOS?
Verified Customer
Verified customer
Asked: 2019-10-30
Answer
The immunogen of M00297-4 anti-C-FOS antibody is Full length recombinant human protein expressed in and purified from E. coli. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-10-30
Question
We are currently using anti-C-FOS antibody M00297-4 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-27
Answer
The anti-C-FOS antibody (M00297-4) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-27
Question
Does anti-C-FOS antibody M00297-4 work for IHC with colon?
Verified Customer
Verified customer
Asked: 2019-09-02
Answer
According to the expression profile of colon, FOS is highly expressed in colon. So, it is likely that anti-C-FOS antibody M00297-4 will work for IHC with colon.
Boster Scientific Support
Answered: 2019-09-02
Question
Will M00297-4 anti-C-FOS antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
S. Johnson
Verified customer
Asked: 2019-08-02
Answer
As indicated on the product datasheet, M00297-4 anti-C-FOS antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-08-02
Question
Please see the WB image, lot number and protocol we used for colon using anti-C-FOS antibody M00297-4. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-07-31
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-31
Question
My team were satisfied with the WB result of your anti-C-FOS antibody. However we have been able to see positive staining in colon nucleus. endoplasmic reticulum. cytoplasm, using this antibody. Is that expected? Could you tell me where is FOS supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-07-19
Answer
Based on literature, colon does express FOS. Generally FOS expresses in nucleus. endoplasmic reticulum. cytoplasm,. Regarding which tissues have FOS expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 14702039
Leukemic T-cell, Pubmed ID: 19690332
Pancreas, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-07-19
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for colon using anti-C-FOS antibody M00297-4. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-10-24
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-10-24
Question
I see that the anti-C-FOS antibody M00297-4 works with IHC, what is the protocol used to produce the result images on the product page?
H. Zhao
Verified customer
Asked: 2017-05-23
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-05-23
Question
My lab would like to test anti-C-FOS antibody M00297-4 on human colon for research purposes, then I may be interested in using anti-C-FOS antibody M00297-4 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
J. Moore
Verified customer
Asked: 2016-12-02
Answer
The products we sell, including anti-C-FOS antibody M00297-4, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2016-12-02
Question
you antibody using your anti-C-FOS antibody for senescence-associated secretory phenotype (sasp) studies. Has this antibody been tested with western blotting on neuronal cells? We would like to see some validation images before ordering.
E. Jones
Verified customer
Asked: 2016-05-03
Answer
Thank you for your inquiry. This M00297-4 anti-C-FOS antibody is tested on hela cells, stimulated cells, neuronal cells. It is guaranteed to work for IF, IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2016-05-03
Question
We have observed staining in rat pancreas. Are there any suggestions? Is anti-C-FOS antibody supposed to stain pancreas positively?
M. Wu
Verified customer
Asked: 2016-02-11
Answer
According to literature pancreas does express FOS. According to Uniprot.org, FOS is expressed in mucosa of stomach, colon, pancreas, leukemic t-cell, among other tissues. Regarding which tissues have FOS expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 14702039
Leukemic T-cell, Pubmed ID: 19690332
Pancreas, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2016-02-11
Question
I was wanting to use your anti-C-FOS antibody for IHC for human colon on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human colon identification?
L. Miller
Verified customer
Asked: 2015-02-18
Answer
As indicated on the product datasheet, M00297-4 anti-C-FOS antibody has been validated for IF, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human colon in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-02-18
