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Key=description, value=The FOXO1A (Phospho-Ser329) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor FOXO1A (Phospho-Ser329) protein expression profile in cells. The kit can be used for measuring the relative amounts of FOXO1A (Phospho-Ser329) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on FOXO1A (Phospho-Ser329).
Key=short_description, value=The FOXO1A (Phospho-Ser329) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor FOXO1A (Phospho-Ser329) protein expression profile in cells. The kit can be used for measuring the relative amounts of FOXO1A (Phospho-Ser329) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on FOXO1A (Phospho-Ser329).
Key=gene_name, value=FOXO1
Key=synonyms, value=FKHR;forkhead box O1A (FKHR) Forkhead box protein O1A;Forkhead in rhabdomyosarcoma;FOXO1
Key=sample_type, value=Cell lines
Key=kit_components, value=FOXO1A (Phospho-Ser329)
Key=subcellular_localization, value=Cytoplasm. Nucleus. Shuttles between the cytoplasm and nucleus. Largely nuclear in unstimulated cells. In osteoblasts, colocalizes with ATF4 and RUNX2 in the nucleus (By similarity). Insulin-induced phosphorylation at Ser-256 by PKB/AKT1 leads, via stimulation of Thr-24 phosphorylation, to binding of 14-3-3 proteins and nuclear export to the cytoplasm where it is degraded by the ubiquitin-proteosomal pathway. Phosphorylation at Ser-249 by CDK1 disrupts binding of 14-3-3 proteins and promotes nuclear accumulation. Phosphorylation by NLK results in nuclear export. Translocates to the nucleus upon oxidative stress-induced phosphorylation at Ser-212 by STK4/MST1. SGK1-mediated phosphorylation also results in nuclear translocation. Retained in the nucleus under stress stimuli including oxidative stress, nutrient deprivation or nitric oxide. Retained in the nucleus on methylation.
Key=tissue_specificity, value=Ubiquitous.
Key=protein_function, value= Transcription factor that is the main target of insulin signaling and regulates metabolic homeostasis in response to oxidative stress. Binds to the insulin response element (IRE) with consensus sequence 5'-TT[G/A]TTTTG-3' and the related Daf-16 family binding element (DBE) with consensus sequence 5'- TT[G/A]TTTAC-3'. Activity suppressed by insulin. Main regulator of redox balance and osteoblast numbers and controls bone mass. Orchestrates the endocrine function of the skeleton in regulating glucose metabolism. Acts synergistically with ATF4 to suppress osteocalcin/BGLAP activity, increasing glucose levels and triggering glucose intolerance and insulin insensitivity. Also suppresses the transcriptional activity of RUNX2, an upstream activator of osteocalcin/BGLAP. In hepatocytes, promotes gluconeogenesis by acting together with PPARGC1A and CEBPA to activate the expression of genes such as IGFBP1, G6PC and PCK1. Important regulator of cell death acting downstream of CDK1, PKB/AKT1 and SKT4/MST1. Promotes neural cell death. Mediates insulin action on adipose tissue. Regulates the expression of adipogenic genes such as PPARG during preadipocyte differentiation and, adipocyte size and adipose tissue-specific gene expression in response to excessive calorie intake. Regulates the transcriptional activity of GADD45A and repair of nitric oxide- damaged DNA in beta-cells. Required for the autophagic cell death induction in response to starvation or oxidative stress in a transcription-independent manner. Mediates the function of MLIP in cardiomyocytes hypertrophy and cardiac remodeling (By similarity).
Key=storage, value=Store at 4°C for up to 6 months.
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FOXO1A (Phospho-Ser329) Colorimetric Cell-Based ELISA Kit

FOXO1 ELISA kit, phospho-specific, cell-based

The FOXO1A (Phospho-Ser329) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor FOXO1A (Phospho-Ser329) protein expression profile in cells. The kit can be used for measuring the relative amounts of FOXO1A (Phospho-Ser329) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on FOXO1A (Phospho-Ser329).

Product Info Summary

SKU: EKC2072
Size: 1 kit, containing two 96-well plates and all necessary reagents
Reactive Species: Human, Mouse, Rat
Application: ELISA
Sample Types: Cell lines

Product Name

FOXO1A (Phospho-Ser329) Colorimetric Cell-Based ELISA Kit

See all FOXO1 products

SKU/Catalog Number

EKC2072

Size

1 kit, containing two 96-well plates and all necessary reagents

Description

The FOXO1A (Phospho-Ser329) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor FOXO1A (Phospho-Ser329) protein expression profile in cells. The kit can be used for measuring the relative amounts of FOXO1A (Phospho-Ser329) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on FOXO1A (Phospho-Ser329).

Storage & Handling

Store at 4°C for up to 6 months.

Cite This Product

FOXO1A (Phospho-Ser329) Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC2072)

Clonality of Antibodies

See Datasheet for details

Assay Range

> 5000 cells/well

Reactive Species

EKC2072 is reactive to FOXO1 in Human, Mouse, Rat samples

Validated Sample Types

Cell lines

Application Guarantee

EKC2072 is guaranteed for ELISA in Human, Mouse, Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Reagent Quantity Container
96-Well Cell Culture Clear-Bottom Microplate 2 Plates -
10x TBS 24 ml (10x) Clear
Quenching Buffer 24 ml (1x) Clear
Blocking Buffer 50 ml (1x) Clear
15x Wash Buffer 50 ml (15x) Clear
100x Anti-FOXO1A Phospho-Ser329 Antibody (Rabbit Polyclonal) 60 µl (100x) Red
100x Anti-FOXO1A Antibody (Rabbit Polyclonal) 60 µl (100x) Purple
100x Anti-GAPDH Antibody (Mouse Monoclonal) 60 µl (100x) Green
HRP-Conjugated Anti-Rabbit IgG Antibody 12ml (1x) Glass
HRP-Conjugated Anti-Mouse IgG Antibody 12ml (1x) Glass
Primary Antibody Diluent 12 ml (1x) Clear
Ready-to-Use Substrate 12 ml (1x) Brown
Stop Solution 12 ml (1x) Clear
Crystal Violet Solution 12ml (1x) Glass
SDS Solution 24 ml (1x) Clear
Adhesive Plate Seals 2 Seals -

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
  • Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
  • 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
  • Deionized or sterile water
  • Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
  • Graph paper or computer software capable of generating or displaying logarithmic functions
  • Absorbent papers or vacuum aspirator
  • Test tubes or microfuge tubes capable of storing 1 ml
  • Orbital shaker
  • Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)

Gene/Protein Information For FOXO1 (Source: Uniprot.Org, NCBI)

Gene Name

FOXO1

Full Name

Forkhead box protein O1

Weight

69662 MW

Alternative Names

FKHR;forkhead box O1A (FKHR) Forkhead box protein O1A;Forkhead in rhabdomyosarcoma;FOXO1 FOXO1 FKH1, FKHRA, FOXO1 forkhead box O1 forkhead box protein O1|forkhead box protein O1A|forkhead, Drosophila, homolog of, in rhabdomyosarcoma

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on FOXO1, check out the FOXO1 Infographic

FOXO1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for FOXO1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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