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Product Info Summary
| SKU: | EKC1926 |
|---|---|
| Size: | 1 kit, containing one 96-well plate and all necessary reagents |
| Reactive Species: | Human, Mouse |
| Application: | ELISA |
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Product info
Product Name
AFX Colorimetric Cell-Based ELISA
SKU/Catalog Number
EKC1926
Size
1 kit, containing one 96-well plate and all necessary reagents
Description
The AFX Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor AFX protein expression profile in cells. The kit can be used for measuring the relative amounts of AFX in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on AFX.
Storage & Handling
Store at 4°C for up to 6 months.
Cite This Product
AFX Colorimetric Cell-Based ELISA (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC1926)
Clonality of Antibodies
See Datasheet for details
Assay Range
> 5000 cells/well
Reactive Species
EKC1926 is reactive to FOXO4 in Human, Mouse samples
Application Guarantee
EKC1926 is guaranteed for ELISA in Human, Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
| Reagent | Quantity | Container |
|---|---|---|
| 96-Well Cell Culture Clear-Bottom Microplate | 1 Plates | - |
| 10x TBS | 24 ml (10x) | Clear |
| Quenching Buffer | 24 ml (1x) | Clear |
| Blocking Buffer | 50 ml (1x) | Clear |
| 15x Wash Buffer | 50 ml (15x) | Clear |
| 100x Anti-AFX Antibody (Rabbit Polyclonal) | 60 µl (100x) | Purple |
| 100x Anti-GAPDH Antibody (Mouse Monoclonal) | 60 µl (100x) | Green |
| HRP-Conjugated Anti-Rabbit IgG Antibody | 6ml (1x) | Glass |
| HRP-Conjugated Anti-Mouse IgG Antibody | 6ml (1x) | Glass |
| Primary Antibody Diluent | 12 ml (1x) | Clear |
| Ready-to-Use Substrate | 12 ml (1x) | Brown |
| Stop Solution | 12 ml (1x) | Clear |
| Crystal Violet Solution | 6ml (1x) | Glass |
| SDS Solution | 24 ml (1x) | Clear |
| Adhesive Plate Seals | 2 Seals | - |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
- Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
- 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
- Deionized or sterile water
- Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
- Graph paper or computer software capable of generating or displaying logarithmic functions
- Absorbent papers or vacuum aspirator
- Test tubes or microfuge tubes capable of storing 1 ml
- Orbital shaker
- Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
Data Examples, Quality Control Data
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Boster Kit Box
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Western blot analysis of extracts from K562 cells
Protein Target Info & Infographic
Gene/Protein Information For FOXO4 (Source: Uniprot.Org, NCBI)
Gene Name
FOXO4
Full Name
Forkhead box protein O4
Weight
53684 MW
Alternative Names
Forkhead box protein O4;Fork head domain transcription factor AFX1;FOXO4;AFX, AFX1, MLLT7; FOXO4 AFX, AFX1, MLLT7 forkhead box O4 forkhead box protein O4|fork head domain transcription factor AFX1|myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 7
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on FOXO4, check out the FOXO4 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for FOXO4: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For AFX Colorimetric Cell-Based ELISA (EKC1926)
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