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Product Info Summary
| SKU: | A00073 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-Forkhead box protein O1 FOXO1 Antibody
SKU/Catalog Number
A00073
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Forkhead box protein O1 FOXO1 Antibody (Catalog # A00073). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
FOXO1 antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Forkhead box protein O1 FOXO1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00073)
Host
Rabbit
Contents
FOXO1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
FOXO1 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human FOXO1. The immunogen is located within the first 50 amino acids of FOXO1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
ESR1 antibody is human, mouse and rat reactive.
Reactive Species
A00073 is reactive to FOXO1 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
69662 MW
Background of FOXO1
FOXO1, also known as FKHR or ForkHead in Rhabdomyosarcoma, is a 70 kDa protein which is a ubiquitously expressed member of a subfamily of the forkhead homeotic gene family of transcription factors and shuttles between the cytoplasm and nucleus. FOXO transcription factors are key players of cell fate decisions, metabolism, stress resistance, tumor suppression and are regulated by growth factors, oxidative stress or nutrient deprivation. In insulin-responsive tissues, stress or nutrient abundance triggers phosphorylation by PKB/AKT, blocking nuclear translocation and activity. Genetic mutations involving FOXO1A are a cause of alveolar rhabdomyosarcoma (RMS2). Recent studies link the anti-tumor activity of FOXO1 and the process of autophagy.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00073 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
FOXO1 antibody can be used for detection of FOXO1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 4 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of FOXO1 in Hela cell lysate with FOXO1 antibody at 1 μg/mL.
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Immunocytochemistry of FOXO1 in HeLa cells with FOXO1 antibody at 4 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For FOXO1 (Source: Uniprot.org, NCBI)
Gene Name
FOXO1
Full Name
Forkhead box protein O1
Weight
69662 MW
Alternative Names
FKH1, FKHR, FOXO1A, Forkhead box protein O1, Forkhead box protein O1A FOXO1 FKH1, FKHRA, FOXO1 forkhead box O1 forkhead box protein O1|forkhead box protein O1A|forkhead, Drosophila, homolog of, in rhabdomyosarcoma
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FOXO1, check out the FOXO1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FOXO1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Forkhead box protein O1 FOXO1 Antibody (A00073)
Hello CJ!
A00073 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Li L,Zhao H,Chen B,Fan Z,Li N,Yue J,Ye Q.FXR activation alleviates tacrolimus-induced post-transplant diabetes mellitus by regulating renal gluconeogenesis and glucose uptake.J Transl Med.2019 Dec 13;17(1):418.doi:10.1186/s12967-019-02170-5.PMID:31836014;PMCID:PMC6909577.
Species: Mouse
A00073 usage in article: APP:IF, SAMPLE:KIDNEY TISSUE, DILUTION:NA
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