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Product Info Summary
| SKU: | EKC1090 |
|---|---|
| Size: | 1 kit, containing one 96-well plate and all necessary reagents |
| Reactive Species: | Human, Mouse, Rat |
| Application: | ELISA |
| Sample Types: | Cell lines |
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Product info
Product Name
Catenin-alpha1 Colorimetric Cell-Based ELISA Kit
See all Catenin alpha 1 products
SKU/Catalog Number
EKC1090
Size
1 kit, containing one 96-well plate and all necessary reagents
Description
The Catenin-alpha1 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Catenin-alpha1 protein expression profile in cells. The kit can be used for measuring the relative amounts of Catenin-alpha1 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Catenin-alpha1.
Storage & Handling
Store at 4°C for up to 6 months.
Cite This Product
Catenin-alpha1 Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC1090)
Clonality of Antibodies
See Datasheet for details
Assay Range
> 5000 cells/well
Reactive Species
EKC1090 is reactive to CTNNA1 in Human, Mouse, Rat samples
Validated Sample Types
Cell lines
Application Guarantee
EKC1090 is guaranteed for ELISA in Human, Mouse, Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
| Reagent | Quantity | Container |
|---|---|---|
| 96-Well Cell Culture Clear-Bottom Microplate | 1 Plates | - |
| 10x TBS | 24 ml (10x) | Clear |
| Quenching Buffer | 24 ml (1x) | Clear |
| Blocking Buffer | 50 ml (1x) | Clear |
| 15x Wash Buffer | 50 ml (15x) | Clear |
| 100x Anti-Catenin-alpha1 Antibody (Rabbit Polyclonal) | 60 µl (100x) | Purple |
| 100x Anti-GAPDH Antibody (Mouse Monoclonal) | 60 µl (100x) | Green |
| HRP-Conjugated Anti-Rabbit IgG Antibody | 6ml (1x) | Glass |
| HRP-Conjugated Anti-Mouse IgG Antibody | 6ml (1x) | Glass |
| Primary Antibody Diluent | 12 ml (1x) | Clear |
| Ready-to-Use Substrate | 12 ml (1x) | Brown |
| Stop Solution | 12 ml (1x) | Clear |
| Crystal Violet Solution | 6ml (1x) | Glass |
| SDS Solution | 24 ml (1x) | Clear |
| Adhesive Plate Seals | 2 Seals | - |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
- Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
- 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
- Deionized or sterile water
- Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
- Graph paper or computer software capable of generating or displaying logarithmic functions
- Absorbent papers or vacuum aspirator
- Test tubes or microfuge tubes capable of storing 1 ml
- Orbital shaker
- Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
Data Examples, Quality Control Data
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Boster Kit Box
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Western blot analysis of extracts from HeLa cells
Protein Target Info & Infographic
Gene/Protein Information For CTNNA1 (Source: Uniprot.Org, NCBI)
Gene Name
CTNNA1
Full Name
Catenin alpha-1
Weight
100071 MW
Superfamily
vinculin/alpha-catenin family
Alternative Names
102 kDa cadherin-associated protein;Alpha E-catenin;CATNA1;CTN1;Cadherin-associated protein;catenin alpha-1 CTNNA1 CAP102, MDPT2 catenin alpha 1 catenin alpha-1|alpha-E-catenin|catenin (cadherin-associated protein), alpha 1, 102kDa|epididymis secretory sperm binding protein|renal carcinoma NY-REN-13
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CTNNA1, check out the CTNNA1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CTNNA1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Catenin-alpha1 Colorimetric Cell-Based ELISA Kit (EKC1090)
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1 Customer Q&As for Catenin-alpha1 Colorimetric Cell-Based ELISA Kit
Question
Hello: Regarding your product Catenin-alpha1 Colorimetric Cell-Based ELISA Kit, as stated in the datasheet that it has been tested in human, mouse and rat. Would it be possible to test on Xenopus cell? Also would it be possible to have more details about the actual sequence of the immunogen peptide? I look forward to hear from you.
Verified Customer
Verified customer
Asked: 2013-02-05
Answer
The product Catenin-alpha1 Colorimetric Cell-Based ELISA Kit was successfully validated with human, mouse and rat samples, but we never tested yet on Xenopus cell. However, it is likely to react with Xenopus because the N-terminal end of Xenopus protein is quite homologous to the human alpha 1 Catenin protein. Please check this link; (http://www.uniprot.org/uniprot/F7DHT4) (http://www.uniprot.org/uniprot/P35221).
Boster Scientific Support
Answered: 2013-02-05
