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Product Info Summary
| SKU: | A06997S129 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Tetraspanin-8 (S129) TSPAN8 Antibody
View all TSPAN8/TM4SF3 Antibodies
SKU/Catalog Number
A06997S129
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Tetraspanin-8 (S129) TSPAN8 Antibody catalog # A06997S129. Tested in WB,IHC applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Tetraspanin-8 (S129) TSPAN8 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06997S129)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic peptide, corresponding to amino acids 110-160 of Human Tetraspanin-8.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A06997S129 is reactive to TSPAN8 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
26044 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06997S129 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:50-1:200
Validation Images & Assay Conditions
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Figure 2. Immunohistochemistry validation of TSPAN8 using Anti-Tetraspanin-8 (S129) TSPAN8 Antibody (A06997S129).
Immunohistochemistry (IHC) analyzes of Tetraspanin-8 (S129) pAb in paraffin-embedded human tonsil carcinoma tissue at 1:50.For more protocol information of IHC
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Figure 1. Western blotting validation for Anti-Tetraspanin-8 (S129) TSPAN8 Antibody A06997S129
Western blot (WB) analysis of Tetraspanin-8 (S129) polyclonal antibody at 1:500 dillutionLane1:Hct116 whole cell lysate
Lane2:SGC7901 whole cell lysate
Lane3:DLD whole cell lysate
Lane4:The Kidney tissue lysate of Mouse
Lane5:The Intestines tissue lysate of Rat
Lane6:BV2 whole cell lysate
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
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Figure 3. Western blotting validation for Anti-Tetraspanin-8 (S129) TSPAN8 Antibody A06997S129
Western blot (WB) analysis of Tetraspanin-8 (S129) pAb at 1:500 dilutionLane1:CT26 whole cell lysate(40ug)
Lane2:PC12 whole cell lysate(40ug)
Lane3:MCF-7 whole cell lysate(40ug)
Lane4:HepG2 whole cell lysate(40ug)
Lane5:HCT116 whole cell lysate(40ug)
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For TSPAN8 (Source: Uniprot.org, NCBI)
Gene Name
TSPAN8
Full Name
Tetraspanin-8
Weight
26044 MW
Superfamily
tetraspanin (TM4SF) family
Alternative Names
Tetraspanin-8;Tspan-8;Transmembrane 4 superfamily member 3;Tumor-associated antigen CO-029;TSPAN8;TM4SF3; TSPAN8 CO-029, TM4SF3 tetraspanin 8 tetraspanin-8|transmembrane 4 superfamily member 3|tspan-8|tumor-associated CO-029
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TSPAN8, check out the TSPAN8 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TSPAN8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Tetraspanin-8 (S129) TSPAN8 Antibody (A06997S129)
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