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Product Info Summary
| SKU: | A01590-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-PNN/DRSP Antibody Picoband®
SKU/Catalog Number
A01590-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PNN/DRSP Antibody Picoband® catalog # A01590-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PNN/DRSP Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01590-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human PNN/DRSP recombinant protein (Position: A2-L241).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01590-2 is reactive to PNN in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
82 kDa
Calculated molecular weight
34140 MW
Background of Pinin
Pinin is a protein that in humans is encoded by the PNN gene. By yeast 2-hybrid analysis of HeLa cells, followed by sequence analysis, it was found that epitope-tagged human PNN interacted with the serine/arginine (SR)-rich proteins SRP75 (SRSF4; 601940), SRM300 (SRRM2; 606032), and SRRP130 (PNISR; 616653). Truncation analysis revealed that the polyserine/RS domain of PNN and flanking sequences participated in binding to these SR proteins. The 4 proteins colocalized in the nucleus of HCE-T cells, and overexpression of any of these proteins affected the distribution of the others between nuclear speckles and nucleoplasm.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01590-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: human Hela whole cell, human HepG2 whole cell, human HL-60 whole cell, human K562 whole cell, rat liver tissue, rat kidney tissue, mouse Neuro-2a whole cell
FCM: HepG2 cell, HL-60 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of PNN/DRSP using anti-PNN/DRSP antibody (A01590-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human HL-60 whole cell lysates,
Lane 4: human K562 whole cell lysates,
Lane 5: rat liver tissue lysates,
Lane 6: rat kidney tissue lysates,
Lane 7: mouse Neuro-2a whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PNN/DRSP antigen affinity purified polyclonal antibody (Catalog # A01590-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PNN/DRSP at approximately 82KD. The expected band size for PNN/DRSPis at 82KD.
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Figure 2. Flow Cytometry analysis of HepG2 cells using anti-PNN/DRSP antibody (A01590-2).
Overlay histogram showing HepG2 cells stained with A01590-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PNN/DRSP Antibody (A01590-2, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Figure 3. Flow Cytometry analysis of HL-60 cells using anti-PNN/DRSP antibody (A01590-2).
Overlay histogram showing HL-60 cells stained with A01590-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PNN/DRSP Antibody (A01590-2, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For PNN (Source: Uniprot.org, NCBI)
Gene Name
PNN
Full Name
Pinin
Weight
34140 MW
Superfamily
pinin family
Alternative Names
Neutrophil cytosol factor 1; NCF-1; 47 kDa autosomal chronic granulomatous disease protein; 47 kDa neutrophil oxidase factor; NCF-47K; Neutrophil NADPH oxidase factor 1; Nox organizer 2; Nox-organizing protein 2; SH3 and PX domain-containing protein 1A; p47-phox; NCF1; NOXO2; SH3PXD1A PNN DRS, DRSP, SDK3, memA pinin, desmosome associated protein pinin|140 kDa nuclear and cell adhesion-related phosphoprotein|SR-like protein|desmosome-associated protein|domain-rich serine protein|melanoma metastasis clone A protein|neutrophil protein|nuclear protein SDK3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PNN, check out the PNN Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PNN: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PNN/DRSP Antibody Picoband® (A01590-2)
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