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Product Info Summary
| SKU: | A05537 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-ZIC1/2/3 Antibody
SKU/Catalog Number
A05537
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-ZIC1/2/3 Antibody (Catalog # A05537). Tested in WB, IHC applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ZIC1/2/3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05537)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Immunogen
Synthesized peptide derived from internal of human ZIC1/2/3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A05537 is reactive to ZIC1 in Human, Mouse
Reconstitution
Observed Molecular Weight
111 kDa
Calculated molecular weight
48309 MW
Background of ZIC1
Acts as a transcriptional activator. Involved in neurogenesis. Plays important roles in the early stage of organogenesis of the CNS, as well as during dorsal spinal cord development and maturation of the cerebellum. Involved in the spatial distribution of mossy fiber (MF) neurons within the pontine gray nucleus (PGN). Plays a role in the regulation of MF axon pathway choice. Promotes MF migration towards ipsilaterally-located cerebellar territories. May have a role in shear flow mechanotransduction in osteocytes. Retains nuclear GLI1 and GLI3 in the cytoplasm. Binds to the minimal GLI-consensus sequence 5'-TGGGTGGTC-3' By similarity.
Yokota N., Cancer Res. 56:377-383(1996).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05537 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Validation Images & Assay Conditions
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Western blot analysis of Zic1 expression in mouse cerebellum extracts at various time points. Zic1 was detected using rabbit anti-Zic1 Ion exchange chromatography purified polyclonal antibody (Catalog #A05537) at 1:5000. HRP conjugated anti-Rabbit IgG (Chemicon) was used at a 1,000 dilution. Personnel communication, K.H. Herzog.
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Western blot analysis of Zic1 expression in mouse cerebellum extract. Protein extracts were prepared from mouse cerebellum between postnatal day 5 (P5) and P18, as indicated above the lanes. Zic1 was detected using rabbit anti-Zic1 Ion exchange chromatography purified polyclonal antibody (Catalog #A05537). Personnel communication, C. Kurschner.
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Western blot analysis of extracts from Jurkat cells and COLO cells, using ZIC1/2/3 antibody A05537.
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Immunohistochemistry analysis of paraffin-embedded human brain tissue using ZIC1/2/3 antibody A05537.
Protein Target Info & Infographic
Gene/Protein Information For ZIC1 (Source: Uniprot.org, NCBI)
Gene Name
ZIC1
Full Name
Zinc finger protein ZIC 1
Weight
48309 MW
Superfamily
GLI C2H2-type zinc-finger protein family
Alternative Names
Zinc finger protein ZIC 1;Zinc finger protein 201;Zinc finger protein of the cerebellum 1;ZIC1;ZIC, ZNF201; ZIC1 BAIDCS, CRS6, ZIC, ZNF201 Zic family member 1 zinc finger protein ZIC 1|Zic family member 1 (odd-paired homolog, Drosophila)|Zinc finger protein of the cerebellum 1|zinc finger protein 201
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ZIC1, check out the ZIC1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ZIC1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ZIC1/2/3 Antibody (A05537)
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