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Product Info Summary
| SKU: | PA1072-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Sonic Hedgehog/SHH Antibody Picoband®
View all Sonic Hedgehog/Shh Antibodies
SKU/Catalog Number
PA1072-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Sonic Hedgehog/SHH Antibody catalog # PA1072-1. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Sonic Hedgehog/SHH Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1072-1)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human Sonic Hedgehog, identical to the related rat and mouse sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1072-1 is reactive to SHH in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
62-65 kDa
Calculated molecular weight
49607 MW
Background of Sonic Hedgehog/Shh
The mouse, chicken, and zebrafish Shh homologs are highly conserved. SHH expression was not detected in adult tissues examined. However, it was expressed in fetal intestine, liver, lung, and kidney. SHH gene is mapped to 7q. SHH mutations are not a frequent cause of isolated oral clefts.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1072-1 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Positive Control
WB: Rat Liver Tissue, Rat Intestine Tissue, HELA Cell, A549 Cell, SMMC Cell, MM231 Cell
IHC: Rat Brain Tissue, Human Mammary Tissue
Validation Images & Assay Conditions
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Anti-Sonic Hedgehog antibody, PA1072-1, Western blotting
Lane 1: Rat Liver Tissue Lysate
Lane 2: Rat Intestine Tissue Lysate
Lane 3: HELA Cell Lysate
Lane 4: A549 Cell Lysate
Lane 5: SMMC Cell Lysate
Lane 6: MM231 Cell Lysate
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Anti-Sonic Hedgehog antibody, PA1072-1, IHC(P)
IHC(P): Rat Brain Tissue
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Anti-Sonic Hedgehog antibody, PA1072-1, IHC(P)
IHC(P): Human Mammary Tissue
Protein Target Info & Infographic
Gene/Protein Information For SHH (Source: Uniprot.org, NCBI)
Gene Name
SHH
Full Name
Sonic hedgehog protein
Weight
49607 MW
Superfamily
hedgehog family
Alternative Names
Sonic hedgehog protein;SHH;HHG-1;Sonic hedgehog protein N-product;Sonic hedgehog protein C-product;SHH; SHH HHG1, HLP3, HPE3, MCOPCB5, SMMCI, ShhNC, TPT, TPTPS sonic hedgehog signaling molecule sonic hedgehog protein|shh unprocessed N-terminal signaling and C-terminal autoprocessing domains|sonic hedgehog homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SHH, check out the SHH Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SHH: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Sonic Hedgehog/SHH Antibody Picoband® (PA1072-1)
Hello CJ!
PA1072-1 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Cao W,Zhang C,Chen R,Wu Q,Xu R,Zhang L,Zhang X. A Novel Cerebroprotein Hydrolysate, CH1, Ameliorates Chronic Focal Cerebral Ischemia Injury by Promoting White Matter Integrity via the Shh/Ptch-1/Gli-1 Signaling Pathway.Neuropsychiatr Dis Treat.2020 Dec 23
Species: Mouse
PA1072-1 usage in article: APP:WB, SAMPLE:BRAIN TISSUE, DILUTION:1:1000
Dai J, Ai K, Du Y, Chen G. Pancreas. 2011 Mar;40(2):233-6. Doi: 10.1097/Mpa.0B013E3181F7E09F. Sonic Hedgehog Expression Correlates With Distant Metastasis In Pancreatic Adenocarcinoma.
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Customer Reviews
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Customer Q&As
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17 Customer Q&As for Anti-Sonic Hedgehog/SHH Antibody Picoband®
Question
Is this PA1072-1 anti-Sonic Hedgehog/SHH antibody reactive to the isotypes of SHH?
Verified Customer
Verified customer
Asked: 2020-05-08
Answer
The immunogen of PA1072-1 anti-Sonic Hedgehog/SHH antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human Sonic Hedgehog(203-218aa ATVHLEQGGTKLVKDL), identical to the related rat and mouse sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-05-08
Question
I am interested in using your anti-Sonic Hedgehog/SHH antibody for midbrain development studies. Has this antibody been tested with western blotting on rat liver tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-04-13
Answer
We appreciate your inquiry. This PA1072-1 anti-Sonic Hedgehog/SHH antibody is validated on rat liver tissue, tissue lysate, intestine tissue, hela cell lysate, a549 cell lysate, smmc cell lysate, mm231 cell lysate, brain tissue, human mammary tissue. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-04-13
Question
We want to test anti-Sonic Hedgehog/SHH antibody PA1072-1 on human fetal lung for research purposes, then I may be interested in using anti-Sonic Hedgehog/SHH antibody PA1072-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-04-02
Answer
The products we sell, including anti-Sonic Hedgehog/SHH antibody PA1072-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-04-02
Question
Does PA1072-1 anti-Sonic Hedgehog/SHH antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
P. Patel
Verified customer
Asked: 2020-02-18
Answer
It shows on the product datasheet, PA1072-1 anti-Sonic Hedgehog/SHH antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-02-18
Question
Please see the WB image, lot number and protocol we used for fetal lung using anti-Sonic Hedgehog/SHH antibody PA1072-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-02-10
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-10
Question
We ordered your anti-Sonic Hedgehog/SHH antibody for IHC on plasma a few years ago. I am using mouse, and We are going to use the antibody for WB next. My question regards examining plasma as well as fetal lung in our next experiment. Could give a recommendation on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2020-01-29
Answer
I have checked the website and datasheets of our anti-Sonic Hedgehog/SHH antibody and it seems that PA1072-1 has been validated on mouse in both IHC and WB. Thus PA1072-1 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-01-29
Question
Will anti-Sonic Hedgehog/SHH antibody PA1072-1 work on goat WB with plasma?
Verified Customer
Verified customer
Asked: 2019-12-11
Answer
Our lab technicians have not tested anti-Sonic Hedgehog/SHH antibody PA1072-1 on goat. You can run a BLAST between goat and the immunogen sequence of anti-Sonic Hedgehog/SHH antibody PA1072-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat plasma in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-11
Question
Our lab were content with the WB result of your anti-Sonic Hedgehog/SHH antibody. However we have observed positive staining in fetal lung sonic hedgehog protein n-product: cell using this antibody. Is that expected? Could you tell me where is SHH supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-10-21
Answer
According to literature, fetal lung does express SHH. Generally SHH expresses in sonic hedgehog protein n-product: cell. Regarding which tissues have SHH expression, here are a few articles citing expression in various tissues:
Fetal lung, Pubmed ID: 7590746
Plasma, Pubmed ID: 16335952
Boster Scientific Support
Answered: 2019-10-21
Question
I was wanting to use your anti-Sonic Hedgehog/SHH antibody for IHC for human fetal lung on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human fetal lung identification?
Verified Customer
Verified customer
Asked: 2019-08-30
Answer
It shows on the product datasheet, PA1072-1 anti-Sonic Hedgehog/SHH antibody has been tested for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human fetal lung in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-30
Question
Does anti-Sonic Hedgehog/SHH antibody PA1072-1 work for IHC with fetal lung?
Verified Customer
Verified customer
Asked: 2018-08-27
Answer
According to the expression profile of fetal lung, SHH is highly expressed in fetal lung. So, it is likely that anti-Sonic Hedgehog/SHH antibody PA1072-1 will work for IHC with fetal lung.
Boster Scientific Support
Answered: 2018-08-27
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for fetal lung using anti-Sonic Hedgehog/SHH antibody PA1072-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-02-13
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-02-13
Question
My question regarding product PA1072-1, anti-Sonic Hedgehog/SHH antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-06-07
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1072-1 anti-Sonic Hedgehog/SHH antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-06-07
Question
Do you have a BSA free version of anti-Sonic Hedgehog/SHH antibody PA1072-1 available?
Verified Customer
Verified customer
Asked: 2017-05-22
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Sonic Hedgehog/SHH antibody PA1072-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-05-22
Question
Is a blocking peptide available for product anti-Sonic Hedgehog/SHH antibody (PA1072-1)?
R. Patel
Verified customer
Asked: 2016-06-22
Answer
We do provide the blocking peptide for product anti-Sonic Hedgehog/SHH antibody (PA1072-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2016-06-22
Question
I see that the anti-Sonic Hedgehog/SHH antibody PA1072-1 works with IHC, what is the protocol used to produce the result images on the product page?
T. Thomas
Verified customer
Asked: 2015-10-14
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2015-10-14
Question
We are currently using anti-Sonic Hedgehog/SHH antibody PA1072-1 for mouse tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on dog tissues as well?
J. Zhao
Verified customer
Asked: 2015-07-23
Answer
The anti-Sonic Hedgehog/SHH antibody (PA1072-1) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-07-23
Question
We have seen staining in rat fetal lung. Any tips? Is anti-Sonic Hedgehog/SHH antibody supposed to stain fetal lung positively?
C. Jones
Verified customer
Asked: 2013-08-01
Answer
According to literature fetal lung does express SHH. According to Uniprot.org, SHH is expressed in left adrenal gland, fetal lung, plasma, among other tissues. Regarding which tissues have SHH expression, here are a few articles citing expression in various tissues:
Fetal lung, Pubmed ID: 7590746
Plasma, Pubmed ID: 16335952
Boster Scientific Support
Answered: 2013-08-01
