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Product Info Summary
| SKU: | M00754-3 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-ZAP70 Monoclonal Antibody
SKU/Catalog Number
M00754-3
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-ZAP70 Monoclonal Antibody catalog # M00754-3. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ZAP70 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00754-3)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ACAF-26
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human ZAP70 The Syk family protein tyrosine kinase Zap-70 is expressed in T and NK cells and plays a critical role in mediating T cell activation in response to T cell receptor (TCR) engagement. Following TCR engagement, Zap-70 is rapidly phosphorylated on several tyrosine residues through autophosphorylation and transphosphorylation by the Src family tyrosine kinase Lck.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00754-3 is reactive to ZAP70 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
100 kDa
Calculated molecular weight
48337 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00754-3 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:1000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Positive Control
WB: Jurkat cell
Validation Images & Assay Conditions
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Western blot analysis of ZAP70 expression in Jurkat cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For ZAP70 (Source: Uniprot.org, NCBI)
Gene Name
ZAP70
Full Name
Tyrosine-protein kinase ZAP-70
Weight
48337 MW
Superfamily
protein kinase superfamily
Alternative Names
G2/mitotic-specific cyclin-B1;CCNB1;CCNB; ZAP70 ADMIO2, IMD48, SRK, STCD, STD, TZK, ZAP-70 zeta chain of T cell receptor associated protein kinase 70 tyrosine-protein kinase ZAP-70|70 kDa zeta-associated protein|70 kDa zeta-chain associated protein|syk-related tyrosine kinase|zeta chain of T cell receptor associated protein kinase 70kDa|zeta-chain (TCR) associated protein kinase 70kDa|zeta-chain associated protein kinase, 70kD
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ZAP70, check out the ZAP70 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ZAP70: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ZAP70 Monoclonal Antibody (M00754-3)
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