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Product Info Summary
| SKU: | EKC2137 |
|---|---|
| Size: | 1 kit, containing two 96-well plates and all necessary reagents |
| Reactive Species: | Human, Mouse |
| Application: | ELISA |
| Sample Types: | Cell lines |
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Product info
Product Name
ZAP-70 (Phospho-Tyr315) Colorimetric Cell-Based ELISA Kit
SKU/Catalog Number
EKC2137
Size
1 kit, containing two 96-well plates and all necessary reagents
Description
The ZAP-70 (Phospho-Tyr315) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor ZAP-70 (Phospho-Tyr315) protein expression profile in cells. The kit can be used for measuring the relative amounts of ZAP-70 (Phospho-Tyr315) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on ZAP-70 (Phospho-Tyr315).
Storage & Handling
Store at 4°C for up to 6 months.
Cite This Product
ZAP-70 (Phospho-Tyr315) Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC2137)
Clonality of Antibodies
See Datasheet for details
Assay Range
> 5000 cells/well
Reactive Species
EKC2137 is reactive to ZAP70 in Human, Mouse samples
Validated Sample Types
Cell lines
Application Guarantee
EKC2137 is guaranteed for ELISA in Human, Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
| Reagent | Quantity | Container |
|---|---|---|
| 96-Well Cell Culture Clear-Bottom Microplate | 2 Plates | - |
| 10x TBS | 24 ml (10x) | Clear |
| Quenching Buffer | 24 ml (1x) | Clear |
| Blocking Buffer | 50 ml (1x) | Clear |
| 15x Wash Buffer | 50 ml (15x) | Clear |
| 100x Anti-ZAP-70 Phospho-Tyr315 Antibody (Rabbit Polyclonal) | 60 µl (100x) | Red |
| 100x Anti-ZAP-70 Antibody (Rabbit Polyclonal) | 60 µl (100x) | Purple |
| 100x Anti-GAPDH Antibody (Mouse Monoclonal) | 60 µl (100x) | Green |
| HRP-Conjugated Anti-Rabbit IgG Antibody | 12ml (1x) | Glass |
| HRP-Conjugated Anti-Mouse IgG Antibody | 12ml (1x) | Glass |
| Primary Antibody Diluent | 12 ml (1x) | Clear |
| Ready-to-Use Substrate | 12 ml (1x) | Brown |
| Stop Solution | 12 ml (1x) | Clear |
| Crystal Violet Solution | 12ml (1x) | Glass |
| SDS Solution | 24 ml (1x) | Clear |
| Adhesive Plate Seals | 2 Seals | - |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
- Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
- 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
- Deionized or sterile water
- Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
- Graph paper or computer software capable of generating or displaying logarithmic functions
- Absorbent papers or vacuum aspirator
- Test tubes or microfuge tubes capable of storing 1 ml
- Orbital shaker
- Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
Data Examples, Quality Control Data
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Boster Kit Box
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Western blot analysis of extracts from Jurkat cells treated with Ca+ 40nM 30'
Protein Target Info & Infographic
Gene/Protein Information For ZAP70 (Source: Uniprot.Org, NCBI)
Gene Name
ZAP70
Full Name
Tyrosine-protein kinase ZAP-70
Weight
69872 MW
Superfamily
protein kinase superfamily
Alternative Names
70 kDa zeta-associated protein;SRK;Syk-related tyrosine kinase;ZA70;ZAP-70;ZAP70;kinase ZAP70 ZAP70 ADMIO2, IMD48, SRK, STCD, STD, TZK, ZAP-70 zeta chain of T cell receptor associated protein kinase 70 tyrosine-protein kinase ZAP-70|70 kDa zeta-associated protein|70 kDa zeta-chain associated protein|syk-related tyrosine kinase|zeta chain of T cell receptor associated protein kinase 70kDa|zeta-chain (TCR) associated protein kinase 70kDa|zeta-chain associated protein kinase, 70kD
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on ZAP70, check out the ZAP70 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for ZAP70: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For ZAP-70 (Phospho-Tyr315) Colorimetric Cell-Based ELISA Kit (EKC2137)
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