Anti-Vinculin/VCL Antibody Picoband®

Vinculin antibody

Boster Bio Anti-Vinculin/VCL Antibody Picoband® catalog # A01207-1. Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance. Cited in 3 publication(s).

Product Info Summary

SKU: A01207-1
Size: 100 μg/vial
Reactive Species: Human, Monkey, Mouse, Rat
Host: Rabbit
Application: ELISA, Flow Cytometry, IF, IHC, WB

Product Name

Anti-Vinculin/VCL Antibody Picoband®

View all Vinculin Antibodies

SKU/Catalog Number

A01207-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Vinculin/VCL Antibody Picoband® catalog # A01207-1. Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Vinculin/VCL Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01207-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human Vinculin/VCL recombinant protein (Position: M1-E149).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A01207-1 is reactive to VCL in Human, Monkey, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

124 kDa

Calculated molecular weight

123799 MW

Background of Vinculin

In mammalian cells, vinculin is a membrane-cytoskeletal protein in focal adhesion plaques that is involved in linkage of integrin adhesion molecules to the actin cytoskeleton. It is mapped to 10q22.2. Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A01207-1 is guaranteed for ELISA, Flow Cytometry, IF, IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.25μg/ml, Human, Mouse, Rat, Monkey
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
Immunofluorescence, 2μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
ELISA, 0.1-0.5μg/ml, -

Positive Control

WB: human Hela whole cell, human U-87MG whole cell, human HT1080 whole cell, human HEK293 whole cell, human SK-OV-3 whole cell, monkey COS-7 whole cell, human K562 whole cell, human SGC-7901 whole cell, rat heart tissue, mouse heart tissue
IHC: human lung cancer tissue, human lung cancer tissue, human mammary cancer tissue
IF: human mammary cancer tissue
FCM: Hela cell

Validation Images & Assay Conditions

Gene/Protein Information For VCL (Source: Uniprot.org, NCBI)

Gene Name

VCL

Full Name

Vinculin

Weight

123799 MW

Superfamily

vinculin/alpha-catenin family

Alternative Names

Vinculin; Metavinculin; MV; VCL VCL CMD1W, CMH15, HEL114, MV, MVCL vinculin vinculin|epididymis luminal protein 114|epididymis secretory sperm binding protein|meta-vinculin|metavinculin|vinculin isoform meta-VCL

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on VCL, check out the VCL Infographic

VCL infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for VCL: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

A01207-1 has been cited in 3 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Toxicity analysis of various Pluronic F-68-coated carbon nanotubes on mesenchymal stem cells

Monitoring of TGF-β 1-Induced Human Lung Adenocarcinoma A549 Cells Epithelial-Mesenchymal Transformation Process by Measuring Cell Adhesion Force with a Microfluidic Device

Dong Y,Chen Y,Zhu D,Shi K,Ma C,Zhang W,Rocchi P,Jiang L,Liu X.Self-assembly of amphiphilic phospholipid peptide dendrimer-based nanovectors for effective delivery of siRNA therapeutics in prostate cancer therapy.J Control Release.2020 Jun 10;322:416-425.d
Species: Human,Mouse
A01207-1 usage in article: APP:WB, SAMPLE:PC-3 CELL, DILUTION:NA

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Order DetailsPrice
A01207-1

100μg

$370
A01207-1-10ug

10μg sample (liquid)

$99
A01207-1-Biotin

100 μg Biotin conjugated

$570
A01207-1-Cy3

100 μg Cy3 conjugated

$570
A01207-1-Dylight488

100 μg Dylight488 conjugated

$570
A01207-1-Dylight550

100 μg Dylight550 conjugated

$570
A01207-1-Dylight594

100 μg Dylight594 conjugated

$570
A01207-1-FITC

100 μg FITC conjugated

$570
A01207-1-HRP

100 μg HRP conjugated

$570
A01207-1-APC

100 μg APC conjugated

$670
A01207-1-PE

100 μg PE conjugated

$670
A01207-1-iFluor647

100 μg iFluor647 conjugated

$670
A01207-1-carrier-free

Carrier Free

$370

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A01207-1
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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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